Optimization of a Nile Red method for rapid lipid determination in autotrophic, marine microalgae is species dependent.


:Several studies have been conducted to develop rapid methods for quantification of lipid content in microalgae, as an alternative for time consuming gravimetric methods. Different studies showed that lipid staining with Nile Red in whole cell suspensions and subsequently quantification by the use of a spectrofluorometric device is a promising method, but a profound optimization and validation is rare. It has already been proven that the correlation curve for quantification is species dependent, but it has not yet been investigated whether this is also the case for the optimization of the Nile Red assay protocol. Therefore, two autotrophic, marine microalgae, Nannochloropsis oculata and T-Isochrysis lutea, strongly differing in e.g. cell wall structure, were selected in this study to investigate whether optimization of the Nile Red assay is species dependent. Besides this, it was checked for one of these species, Nannochloropsis, whether the lipid content, determined by the Nile Red assay, could indeed be correlated with the neutral and/or total lipid content determined by gravimetric methods. It was found that optimization of the Nile Red assay was strongly species dependent. Consequently, optimization has to be done for each species before using the assay. For Nannochloropsis, a good correlation was found between total and neutral lipid content obtained by the Nile Red assay and by gravimetric methods.


J Microbiol Methods


Balduyck L,Veryser C,Goiris K,Bruneel C,Muylaert K,Foubert I




Has Abstract


2015-11-01 00:00:00












  • Development of a rapid detection and enumeration method for thermophilic bacilli in milk powders.

    abstract::Thermophilic strains of Geobacillus, Anoxybacillus and Bacillus that are able to grow at 55 degrees C and above are recognized as commonly occurring contaminants during the production of milk powders. In particular, Anoxybacillus flavithermus strain C and Bacillus licheniformis strain F are often the most prevalent. W...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Rueckert A,Ronimus RS,Morgan HW

    更新日期:2005-02-01 00:00:00

  • Use of competitive DNA hybridization to identify differences in the genomes of bacteria.

    abstract::Although recent technological advances in DNA sequencing and computational biology now allow scientists to compare entire microbial genomes, comparisons of closely related bacterial species and individual isolates by whole-genome sequencing approaches remains prohibitively expensive for most laboratories. Here we repo...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Shanks OC,Santo Domingo JW,Graham JE

    更新日期:2006-08-01 00:00:00

  • Integration of biological pre-treatment methods for increased energy recovery from paper and pulp biosludge.

    abstract::The paper and pulp industry (PPI) produces high quantities of solid and liquid discharge and is regarded as the most polluting industry in the world causing adverse effects to environments and human beings. Hence changes in the way PPI sludge and waste materials are treated is urgently required. Nearly, 10 million ton...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Chakraborty D,Shelvapulle S,Reddy KR,Kulkarni RV,Puttaiahgowda YM,Naveen S,Raghu AV

    更新日期:2019-05-01 00:00:00

  • A tetracycline-inducible expression vector for Streptococcus agalactiae allowing controllable gene expression.

    abstract::Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Lartigue MF,Bouloc P

    更新日期:2014-01-01 00:00:00

  • Use of both 16S rRNA and engineered functional genes with real-time PCR to quantify an engineered, PCB-degrading Rhodococcus in soil.

    abstract::A real-time PCR (RTm-PCR) assay using fluorescently labeled oligonucleotides (TaqMan probes) was used to detect and quantify the recombinant Rhodococcus sp. strain RHA1(fcb) in soil. One primer and probe set targeted a hypervariable region of the 16S rRNA gene unique to strain RHA1(fcb) and its phylogenetic relatives,...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Rodrigues JL,Aiello MR,Urbance JW,Tsoi TV,Tiedje JM

    更新日期:2002-10-01 00:00:00

  • A bench-top automated workstation for nucleic acid isolation from clinical sample types.

    abstract::Systems that automate extraction of nucleic acid from cells or viruses in complex clinical matrices have tremendous value even in the absence of an integrated downstream detector. We describe our bench-top automated workstation that integrates our previously-reported extraction method - TruTip - with our newly-develop...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Thakore N,Garber S,Bueno A,Qu P,Norville R,Villanueva M,Chandler DP,Holmberg R,Cooney CG

    更新日期:2018-05-01 00:00:00

  • Attachment of Staphylococcus aureus to eukaryotic cells and experimental pitfalls in staphylococcal adherence assays: a critical appraisal.

    abstract::Staphylococcus aureus is a bacterial species with pathogenic potential to both humans and animals. The primary natural niche is said to be the human vestibulum nasi from where bacterial cells may spread to the environment or additional anatomical sites such as the perineum or the hands, where residence is usually tran...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章,评审


    authors: Van Belkum A,Kools-Sijmons M,Verbrugh H

    更新日期:2002-01-01 00:00:00

  • Evaluation of RNA degradation in pure culture and field Microcystis samples preserved with various treatments.

    abstract::RNA-based molecular technique (RT-qPCR) is a promising method for microcystin monitoring in lakes and reservoirs, but great lability of RNA in cyanobacterial samples limits its application. To date, no studies have investigated how to effectively preserve RNA in cyanobacterial samples. In this study, four different tr...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Li X,Qiu D,Chen S,Li J,Luo C,Hu D,Li J,Zhu J,Chen H,Li S,Yu X

    更新日期:2019-09-01 00:00:00

  • Strategy for screening metagenomic resources for exocellulase activity using a robotic, high-throughput screening system.

    abstract::Exocellulases play a key role in cleaving the accessible ends of cellulose molecules to release soluble glucose and cellobiose. To date, there have been no screens for exocellulase owing to assay protocol limitations, the high cost of substrates, and low activity of exocellulases compared with endocellulases. This stu...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Ko KC,Han Y,Cheong DE,Choi JH,Song JJ

    更新日期:2013-09-01 00:00:00

  • A new filter-eluting solution that facilitates improved recovery of Cryptosporidium oocysts from water.

    abstract::Cryptosporidium is a zoonotic coccidian parasite associated with diarrhea, and the disinfectant-resistant oocysts are threats to public health even in industrialized countries. In order to make an accurate assessment of the risk to public health, a detection method that has a high recovery rate of oocysts in water is ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Inoue M,Rai SK,Oda T,Kimura K,Nakanishi M,Hotta H,Uga S

    更新日期:2003-12-01 00:00:00

  • Transmission of Chlamydophila pneumoniae from dendritic cells to macrophages does not require cell-to-cell contact in vitro.

    abstract::Chlamydophila pneumoniae (C. pneumoniae) has been detected in macrophages (Mø) and dendritic cells (DC) in vascular diseases. To understand the importance of these cell types in C. pneumoniae infection and transmission, we infected DC and cultivated them with Mø in a coculture model system which precludes cell-to-cell...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Wittkop U,Peppmueller M,Njau F,Leibold W,Klos A,Krausse-Opatz B,Hudson AP,Zeidler H,Haller H,Wagner AD

    更新日期:2008-03-01 00:00:00

  • Quantification of Pseudomonas aeruginosa hydrogen cyanide production by a polarographic approach.

    abstract::Pseudomonas aeruginosa is an opportunistic pathogen responsible for numerous infections acquired in hospital especially in persons whose immune systems are weakened, such as with patient suffering from AIDS or cystic fibrosis. This bacterium produces a great diversity of virulence factors among them hydrogen cyanide (...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Blier AS,Vieillard J,Gerault E,Dagorn A,Varacavoudin T,Le Derf F,Orange N,Feuilloley M,Lesouhaitier O

    更新日期:2012-07-01 00:00:00

  • A comparison of silver ion to streptavidin coated microplates.

    abstract::Direct comparisons are made between covalently linked streptavidin and silver ion coated microplates. Both coatings can immobilize biotinylated molecules. Silver ion coated microplate wells can immobilize 1.8 times higher amounts of biotin labeled horseradish peroxidase. The quantitation range and capacity for the cap...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Bonen MR,García AA,Hoffman SA

    更新日期:2001-03-01 00:00:00

  • Comparative SELDI-TOF-MS profiling of low-molecular-mass proteins from Lignosus rhinocerus (Cooke) Ryvarden grown under stirred and static conditions of liquid fermentation.

    abstract::Mushrooms are considered as important source of biologically active compounds which include low-molecular-mass protein/peptides (LMMP). In this study, we attempted to profile the LMMP from Lignosus rhinocerus, a wild medicinal mushroom, grown by static cultures (SC) and in stirred tank reactor (STR). Crude water extra...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Lau BF,Aminudin N,Abdullah N

    更新日期:2011-10-01 00:00:00

  • Comparative performance of Aspergillus galactomannan ELISA and PCR in sputum from patients with ABPA and CPA.

    abstract:OBJECTIVES:Galactomannan (GM) and Aspergillus DNA detection are useful tools for the diagnosis of invasive pulmonary aspergillosis (IPA), primarily in blood and bronchoscopy samples. This study aimed to evaluate the utility of both markers for detection of Aspergillus in sputum from patients with allergic bronchopulmon...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Fayemiwo S,Moore CB,Foden P,Denning DW,Richardson MD

    更新日期:2017-09-01 00:00:00

  • Detection in coal tar waste-contaminated groundwater of mRNA transcripts related to naphthalene dioxygenase by fluorescent in situ hybridization with tyramide signal amplification.

    abstract::The ideal ecological metabolic activity assay would be applied to naturally occurring microbial populations immediately fixed in the field, and the assay would focus upon intracellular parameters indicative of a dynamic biogeochemical process. In this study, fluorescent in situ hybridization (FISH) with tyramide signa...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Bakermans C,Madsen EL

    更新日期:2002-06-01 00:00:00

  • Development of a novel plasmid vector pTIO-1 adapted for electrotransformation of Porphyromonas gingivalis.

    abstract::We report here the construction of a plasmid vector designed for the efficient electrotransformation of the periodontal pathogen Porphyromonas gingivalis. The novel Escherichia coli-Bacteroides/P. gingivalis shuttle vector, designated pTIO-1, is based on the 11.0-kb E. coli-Bacteroides conjugative shuttle vector, pVAL...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Tagawa J,Inoue T,Naito M,Sato K,Kuwahara T,Nakayama M,Nakayama K,Yamashiro T,Ohara N

    更新日期:2014-10-01 00:00:00

  • Evaluation of urine for Leishmania infantum DNA detection by real-time quantitative PCR.

    abstract::The availability of some sorts of biological samples which require noninvasive collection methods has led to an even greater interest in applying molecular biology on visceral leishmaniasis (VL) diagnosis, since these samples increase the safety and comfort of both patients and health professionals. In this context, t...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Pessoa-E-Silva R,Mendonça Trajano-Silva LA,Lopes da Silva MA,da Cunha Gonçalves-de-Albuquerque S,de Goes TC,Silva de Morais RC,Lopes de Melo F,de Paiva-Cavalcanti M

    更新日期:2016-12-01 00:00:00

  • Wintergreen oil: a novel method in Wheatley's trichrome staining technique.

    abstract::Permanent staining of faecal smears by Wheatley's trichrome technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome staining technique...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Salleh FM,Anuar TS,Yasin AM,Moktar N

    更新日期:2012-10-01 00:00:00

  • A simple and sensitive method to extract bacterial, yeast and fungal DNA from blood culture material.

    abstract::This study investigated the various commercially available kits and 'in-house' methods to extract DNA from Gram-negative and Gram-positive bacteria, yeast and fungal agents in commonly employed blood culture material. The main methods investigated were as follows; Qiagen QIAmp Blood kit, Roche high PCR template prepar...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Millar BC,Jiru X,Moore JE,Earle JA

    更新日期:2000-10-01 00:00:00

  • Development of a novel in vitro onychomycosis model for the evaluation of topical antifungal activity.

    abstract::A novel in vitro onychomycosis model was developed to easily predict the topical activity potential of novel antifungal drugs. The model encompasses drug activity and diffusion through bovine hoof slices in a single experimental set-up. Results correspond well with the antifungal susceptibility assay and Franz cell di...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Sleven R,Lanckacker E,Boulet G,Delputte P,Maes L,Cos P

    更新日期:2015-05-01 00:00:00

  • Epidemiological investigation and glycotyping of clinical Pseudomonas aeruginosa isolates from patients with cystic fibrosis by mass spectrometry: association with multiple drug resistance.

    abstract::The aim of the present investigation was to develop a novel approach for rapid identification and differentiation of Pseudomonas aeruginosa clones from Canadian cystic fibrosis patients by capillary electrophoresis-mass spectrometry. We screened P. aeruginosa isolates for lipopolysaccharide structure and presence/abse...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Altman E,Wang Z,Aaron SD,Liu X,Vandemheen KL,Ferris W,Giesbrecht T,Li J

    更新日期:2009-02-01 00:00:00

  • Isolation and concentration of Salmonellae with an immunoaffinity column.

    abstract::A method for rapidly and selectively isolating Salmonellae from buffer solutions and concentrating the bacteria by a factor of approximately 500 was developed. Anti-Salmonellae antibody was covalently linked to 40 microm polyacrylamide beads to prepare a solid phase with affinity for the bacteria. The beads were packe...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Brewster JD

    更新日期:2003-10-01 00:00:00

  • A chemical proteomics approach to identify c-di-GMP binding proteins in Pseudomonas aeruginosa.

    abstract::In many bacteria, high levels of the ubiquitous second messenger c-di-GMP have been demonstrated to suppress motility and to promote the establishment of surface-adherent biofilm communities. While molecular mechanisms underlying the synthesis and degradation of c-di-GMP have been comprehensively characterized, little...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Düvel J,Bertinetti D,Möller S,Schwede F,Morr M,Wissing J,Radamm L,Zimmermann B,Genieser HG,Jänsch L,Herberg FW,Häussler S

    更新日期:2012-02-01 00:00:00

  • Interrogation of single nucleotide polymorphisms in gnd provides a novel method for molecular serogrouping of clinically important Shiga toxin producing Escherichia coli (STEC) targeted by regulation in the United States, including the "big six" non-O157

    abstract::Escherichia coli O157:H7 has frequently been associated with foodborne infections and is considered an adulterant in raw non-intact beef in the U.S. Shiga toxin-producing E. coli (STEC) belonging to serogroups O26, O45, O103, O111, O121, and O145 (known as the "big six" non-O157) were estimated to cause >70% of foodbo...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Elder JR,Bugarel M,den Bakker HC,Loneragan GH,Nightingale KK

    更新日期:2016-10-01 00:00:00

  • Improved gene disruption method and Cre-loxP mutant system for multiple gene disruptions in Hansenula polymorpha.

    abstract::In H. polymorpha, there is still a lack of a highly efficient gene disruption method. To help address this issue, we presented a simple and efficient method for both single and multiple gene disruptions in H. polymorpha. The knockout system combined a variation of sticky-end polymerase chain reaction method (SEP), spl...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Qian W,Song H,Liu Y,Zhang C,Niu Z,Wang H,Qiu B

    更新日期:2009-12-01 00:00:00

  • Detection of respiratory enzyme activity in Giardia cysts and Cryptosporidium oocysts using redox dyes and immunofluorescence techniques.

    abstract::The fluorescent redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), combined with fluorescein-labeled antibodies, was tested for the simultaneous detection of the respiratory electron transport system (ETS) activity and enumeration of Giardia cysts and Cryptosporidium oocysts by spectral microfluorometry and epi...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Iturriaga R,Zhang S,Sonek GJ,Stibbs H

    更新日期:2001-07-30 00:00:00

  • Construction of a conditional lethal Salmonella mutant via genetic recombination using the ara system and asd gene.

    abstract::In order to construct a conditional lethal Salmonella mutant, an arabinose-regulated recombinant genetic system was used. The Salmonella aspartate semialdehyde dehydrogenase (asd) gene was localized under the control of araC P(araBAD) in a plasmid to create the araC P(araBAD)::asd cassette. The cassette was cloned int...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Kim SW,Kang HY,Hur J,Gal SW,Bang WY,Cho KK,Kim CW,Bahk JD,Lee JH

    更新日期:2011-11-01 00:00:00

  • Enhanced analysis of bacteria susceptibility in connected biofilms.

    abstract::A common method for visualizing bacterial biofilms is through confocal laser scanning microscopy images. Current software packages separate connected-biofilm bacteria from unconnected bacteria, such as planktonic or dispersed bacteria, but do not save both image sequences, making interpretation of the two bacterial po...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Sommerfeld Ross S,Reinhardt JM,Fiegel J

    更新日期:2012-07-01 00:00:00

  • Comparison of PCR and serotyping of Group B Streptococcus in pregnant women: the Oslo GBS-study.

    abstract::Streptococcus agalactiae (GBS) is a leading cause of invasive neonatal infection. Serotyping of GBS is important in following epidemiological trends and vaccine development. Capsular serotyping of GBS by latex agglutination has been the predominant typing method, but more recently capsular genotyping has been introduc...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Brigtsen AK,Dedi L,Melby KK,Holberg-Petersen M,Radtke A,Lyng RV,Andresen LL,Jacobsen AF,Fugelseth D,Whitelaw A

    更新日期:2015-01-01 00:00:00