当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > Comparison of freeze-thaw cycles for nucleic acid extraction and molecular detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in environmental matrices.

Comparison of freeze-thaw cycles for nucleic acid extraction and molecular detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in environmental matrices.

Abstract：

:Freeze-thaw DNA extraction methods and PCR primers were compared to optimize detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in different matrices. Increasing FT cycles did not increase parasite DNA detection, and primers targeting the 18S ssrRNA gene yielded the most sensitive detection of C. parvum oocysts.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Manore AJW,Harper SL,Aguilar B,Weese JS,Shapiro K

doi

10.1016/j.mimet.2018.11.017

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

1-4

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(18)30399-3

journal_volume

156

pub_type

杂志文章

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