Abstract：
:Phytophthora blight of pepper is a completely destructive plant disease caused by the oomycete pathogen Phytophthora capsici Leonian. Phytophthora disease is responsible for major losses in pepper production and the pathogen can survive in soil in the absence of the host plant for many years. Currently, there are no early diagnostic reagents available that specifically target P. capsici. Therefore, diagnostic tools that can detect Phytophthora are required. In the present study, we screened for P. capsici-binding peptides using M13 phage display. After five rounds of biopanning, we identified P. capsici-binding peptides from a random peptide library that showed high binding affinity and specificity toward P. capsici in the picomolar range. These peptides can be used to develop novel diagnostic probes or potent inhibitors with diverse polyvalencies.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Park HY,Park HC,Yoon MYdoi
10.1016/j.mimet.2009.04.006subject
Has Abstractpub_date
2009-07-01 00:00:00pages
54-8issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(09)00114-6journal_volume
78pub_type
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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abstract：:This contribution honoring David C. White (DC) summarizes the five years I interacted with him on a daily basis in his laboratory. Over this time we worked on many different projects all tied together by the unifying principle now recognized as central to bacterial life in nature: biofilms. My goal is to convey some o...
journal_title：Journal of microbiological methods
pub_type： 杂志文章
doi：10.1016/j.mimet.2007.07.003
更新日期：2008-07-01 00:00:00
