Epidemiological investigation and glycotyping of clinical Pseudomonas aeruginosa isolates from patients with cystic fibrosis by mass spectrometry: association with multiple drug resistance.


:The aim of the present investigation was to develop a novel approach for rapid identification and differentiation of Pseudomonas aeruginosa clones from Canadian cystic fibrosis patients by capillary electrophoresis-mass spectrometry. We screened P. aeruginosa isolates for lipopolysaccharide structure and presence/absence of alginate and correlated these findings with antibiotic resistance patterns.


J Microbiol Methods


Altman E,Wang Z,Aaron SD,Liu X,Vandemheen KL,Ferris W,Giesbrecht T,Li J




Has Abstract


2009-02-01 00:00:00














  • Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis.

    abstract::Bacterial community structure was studied in a Flevo silt loam (FSL) soil microplot, as well as in 15 other soils, by using DNA extraction followed by molecular fingerprinting. Total community DNA was extracted and purified by a direct method, which yielded amplifiable DNA of high molecular weight for all soils. A var...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Gelsomino A,Keijzer-Wolters AC,Cacco G,van Elsas JD

    更新日期:1999-10-01 00:00:00

  • Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system.

    abstract::A rapid bead assay for detecting pathogenic bacteria with a simple microfluidic chip-based system was developed. Five oligonucleotide probes corresponding to the 16S rRNA of the targeted bacteria were coupled covalently to fluorescent beads. Four species of bacteria (Escherichia coli, Salmonella enterica subsp. enteri...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Ikeda M,Yamaguchi N,Tani K,Nasu M

    更新日期:2006-11-01 00:00:00

  • Preoperative urine culture - Is it necessary to prevent infectious complications following ureterorenoscopy?

    abstract::To prevent postoperative infectious complications, a urinary tract infection should be either diagnostically excluded or treated prior to ureterorenoscopy (URS). URS is a frequently performed endoscopic surgery for urological stone removal. Although the urinary dipstick test represents a simple and cost-effective meth...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Grüne B,Viehweger V,Waldbillig F,Nientiedt M,Kriegmair MC,Rassweiler-Seyfried MC,Ritter M

    更新日期:2020-06-01 00:00:00

  • Isolation of Harveyi clade Vibrio spp. collected in aquaculture farms: How can the identification issue be addressed?

    abstract::Aquaculture is a fast growing industry with its development hampered by bacterial diseases. Vibriosis caused by Harveyi clade strains is known for causing heavy loss especially in shrimp aquaculture farms. For farm treatment and pathogen spread management, veterinarians and researchers need reliable bacterial identifi...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Culot A,Grosset N,Bruey Q,Auzou M,Giard JC,Favard B,Wakatsuki A,Baron S,Frouel S,Techer C,Gautier M

    更新日期:2021-01-01 00:00:00

  • Adhesion of Yersinia enterocolitica to non-cultured epithelial cells from pig and rabbit ilea.

    abstract::A simple and reliable method was developed to isolate intact epithelial cells from pig and rabbit ilea and these were used to investigate the adhesion of Yersinia enterocolitica. Hydrophobic interaction was eliminated by treating the bacterial culture with 0.8 M tetramethyl urea (TMU). Virulent strains of Y. enterocol...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Hussein HM,Fenwick SG,Lumsden JS

    更新日期:2007-02-01 00:00:00

  • Establishment of a semi-automated pathogen DNA isolation from whole blood and comparison with commercially available kits.

    abstract::Molecular methods for bacterial pathogen identification are gaining increased importance in routine clinical diagnostic laboratories. Achieving reliable results using DNA based technologies is strongly dependent on pre-analytical processes including isolation of target cells and their DNA of high quality and purity. I...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Wiesinger-Mayr H,Jordana-Lluch E,Martró E,Schoenthaler S,Noehammer C

    更新日期:2011-06-01 00:00:00

  • Polymerase chain reaction fingerprints of methicillin-resistant Staphylococcus aureus clinical isolates in Greece are related to certain antibiotypes.

    abstract::Characterization of clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and representatives of three MRSA clones from other hospitals was performed by arbitrarily primed polymerase chain reaction (AP-PCR) and antibiotic susceptibility patterns. All isolates were mecA-positive and two main antibioty...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Bartzavali-Louki C,Dimitracopoulos G,Spiliopoulou I

    更新日期:2003-06-01 00:00:00

  • A robust and fast bacteria counting method using CdSe/ZnS core/shell quantum dots as labels.

    abstract::A rapid, sensitive fluorescence measurement method for detecting the bacterial count using CdSe/ZnS as a fluorescence marker was described. High-quality CdSe/ZnS nanocrystals were synthesized and successfully conjugated with bacteria. The fluorescence intensity was proportional to bacterial count in the range of 10(2)...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Fu X,Huang K,Liu S

    更新日期:2009-12-01 00:00:00

  • High molecular weight DNA recovery from soils prerequisite for biotechnological metagenomic library construction.

    abstract::Soil is a complex environment considered as one of the main reservoirs of microbial diversity. However, the inability to cultivate most soil bacteria hampered fundamental attempts to determine the diversity of the prokaryotic world and limited its industrial exploitation. In the last 20 years, new methods have been de...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Bertrand H,Poly F,Van VT,Lombard N,Nalin R,Vogel TM,Simonet P

    更新日期:2005-07-01 00:00:00

  • 3D-printed microfluidic magnetic preconcentrator for the detection of bacterial pathogen using an ATP luminometer and antibody-conjugated magnetic nanoparticles.

    abstract::Various types of microfluidic systems have been developed to detect bacterial pathogens. However, most of these require enrichment steps that take at least several hours when detecting bacteria that are present with a low number of cells and, in addition, fabrication requires complicated assembly steps. In this study,...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Park C,Lee J,Kim Y,Kim J,Lee J,Park S

    更新日期:2017-01-01 00:00:00

  • Multivariate analysis of bacterial volatile compound profiles for discrimination between selected species and strains in vitro.

    abstract::Volatile compounds (VCs) are produced by all microorganisms as part of their normal metabolism. The aim of this study was to determine whether bacterial VC profiles could be used to discriminate between selected bacterial species and strains in vitro. Selected Ion Flow Tube Mass Spectrometry (SIFT-MS) was used to quan...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Thorn RM,Reynolds DM,Greenman J

    更新日期:2011-02-01 00:00:00

  • A new high-throughput AFLP approach for identification of new genetic polymorphism in the genome of the clonal microorganism Mycobacterium tuberculosis.

    abstract::We have here applied high-throughput amplified fragment length polymorphism (htAFLP) analysis to strains belonging to the five classical species of the Mycobacterium tuberculosis complex. Using 20 strains, three enzyme combinations and eight selective amplification primer pairs, 24 AFLP reactions were performed per st...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: van den Braak N,Simons G,Gorkink R,Reijans M,Eadie K,Kremers K,van Soolingen D,Savelkoul P,Verbrugh H,van Belkum A

    更新日期:2004-01-01 00:00:00

  • Development of a method for heterologous gene expression in Enterobacter amnigenus, a potential host for the biological control of mosquito larvi.

    abstract::An integrative plasmid containing a 1.3 kb fragment of chromosomal DNA from Enterobacter amnigenus was constructed. The Omega fragment encoding spectinomycin/streptomycin resistance was cloned into the unique BglII site of the resulting plasmid, and the interrupted fragment was transferred via plasmid pMAK705 by elect...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Nantapong N,Tanapongpipat S,Cole J,Panyim S

    更新日期:2002-05-01 00:00:00

  • Preservation of Malassezia pachydermatis using skimmed milk as cryoprotective agent.

    abstract::This study evaluated the effect of skimmed milk as a cryoprotectant for the maintenance and long-term preservation of 70 Malassezia pachydermatis strains. An initial suspension of each strain was prepared in sterile distilled water with skimmed milk. The most effective method was cryopreservation that ensured 100% via...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Silva Sergent FA,Acosta-Hernández B,Raduán Jaber J,Rosario Medina I,Ferrer Quintana O,Real Valcárcel F,Padilla Castillo D,Déniz Suárez S

    更新日期:2017-01-01 00:00:00

  • A novel recombinant multiepitope protein candidate for the diagnosis of brucellosis: A pilot study.

    abstract::Brucellosis is a zoonotic disease transmitted to humans from infected animals. As a systemic disease, it can harm any organ or system of the host body. Human brucellosis presents with various clinical symptoms, which makes diagnosis challenging. Serological diagnosis of brucellosis is based on ELISA or agglutination t...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Yin D,Bai Q,Zhang J,Xu K,Li J

    更新日期:2020-07-01 00:00:00

  • Comparative SELDI-TOF-MS profiling of low-molecular-mass proteins from Lignosus rhinocerus (Cooke) Ryvarden grown under stirred and static conditions of liquid fermentation.

    abstract::Mushrooms are considered as important source of biologically active compounds which include low-molecular-mass protein/peptides (LMMP). In this study, we attempted to profile the LMMP from Lignosus rhinocerus, a wild medicinal mushroom, grown by static cultures (SC) and in stirred tank reactor (STR). Crude water extra...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Lau BF,Aminudin N,Abdullah N

    更新日期:2011-10-01 00:00:00

  • Quantification of homogentisate-1,2-dioxygenase expression in a fungus degrading ethylbenzene.

    abstract::A quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) assay was utilized to quantify the expression of ElHDO in the fungus Exophiala lecanii-corni during the biodegradation of ethylbenzene and other volatile organic pollutants. The assay was applied to measure the impact of pollutant mixtu...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Gunsch CK,Kinney KA,Szaniszlo PJ,Whitman CP

    更新日期:2006-11-01 00:00:00

  • Quantification of Pseudomonas aeruginosa hydrogen cyanide production by a polarographic approach.

    abstract::Pseudomonas aeruginosa is an opportunistic pathogen responsible for numerous infections acquired in hospital especially in persons whose immune systems are weakened, such as with patient suffering from AIDS or cystic fibrosis. This bacterium produces a great diversity of virulence factors among them hydrogen cyanide (...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Blier AS,Vieillard J,Gerault E,Dagorn A,Varacavoudin T,Le Derf F,Orange N,Feuilloley M,Lesouhaitier O

    更新日期:2012-07-01 00:00:00

  • A reverse-hybridization test for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs.

    abstract::The S. PneumoStrip test is a recently developed reverse hybridization strip-based commercial assay that allows for the identification of 76 pneumococcal serotypes, 42 individually and 34 in pairs, according to their specific gene sequences. The test was validated with reference strains of 92 different pneumococcal ser...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Marimón JM,Morales M,Gamen S,Manrique A,Ercibengoa M,Cilla G

    更新日期:2017-12-01 00:00:00

  • A toolbox to measure changes in the cell wall glycopolymer composition during differentiation of Streptomyces coelicolor A3(2).

    abstract::Cell wall glycopolymers (CWG) represent an important component of the Gram-positive cell envelope with many biological functions. The mycelial soil bacterium Streptomyces coelicolor A3(2) incorporates two distinct CWGs, polydiglycosylphosphate (PDP) and teichulosonic acid, into the cell wall of its vegetative mycelium...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Sigle S,Steblau N,Wohlleben W,Muth G

    更新日期:2016-09-01 00:00:00

  • Optimization of media components for production of antimicrobial compound by Brevibacillus brevis EGS9 isolated from mangrove ecosystem.

    abstract::The aim of the present study was to show the efficiency of multi-factorial experimental design to investigate factors affecting the antimicrobial compound production by Brevibacillus brevis EGS9 isolated from mangrove sediment soil. The isolate utilizing different carbon and nitrogen sources showed different growth ch...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: T A,P SK

    更新日期:2017-11-01 00:00:00

  • Sterilization of biological pathogens using supercritical fluid carbon dioxide containing water and hydrogen peroxide.

    abstract::Novel noninvasive techniques for the removal of biological contaminants to generate clean or sterile materials are in demand by the medical, pharmaceutical and food industries. The sterilization method described here uses supercritical fluid carbon dioxide (SF-CO(2)) containing 3.3% water and 0.1% hydrogen peroxide (v...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Checinska A,Fruth IA,Green TL,Crawford RL,Paszczynski AJ

    更新日期:2011-10-01 00:00:00

  • Flow cytometry assessment of bacterioplankton in tropical marine environments.

    abstract::Flow cytometry was used to characterize bacterioplankton from two tropical environments in Brazil: the eutrophic Guanabara Bay and the oligotrophic southwest Atlantic Ocean. Bacterial abundance was evaluated by flow cytometry, and cells were stained with SYTO 13, allowing demonstration of differences in nucleic acid c...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Andrade L,Gonzalez AM,Araujo FV,Paranhos R

    更新日期:2003-12-01 00:00:00

  • BEEP: An assay to detect bio-energetic and envelope permeability alterations in Pseudomonas aeruginosa.

    abstract::We developed an effective and rapid assay to detect both bio-energetic and envelope permeability (BEEP) alterations of Pseudomonas aeruginosa. The assay is based on quantification of extracellular ATP in bacterial cultures using luciferase as a reporter. To demonstrate the validity of our assay we conducted a biased s...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Suh SJ,Shuman J,Carroll LP,Silo-Suh L

    更新日期:2016-06-01 00:00:00

  • Electroporation as a tool to transfer the plasmid pRL489 in Oscillatoria MKU 277.

    abstract::To establish the gene transfer system in the cyanobacteria, we successfully introduced the shuttle plasmid pRL489 into Oscillatoria MKU 277 by electroporation with transformation frequencies of up to 102 cfu/mug of plasmid DNA. These findings suggest that Oscillatoria MKU 277 can be used as an experimental tool for ge...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Ravindran CR,Suguna S,Shanmugasundaram S

    更新日期:2006-07-01 00:00:00

  • Sensitivity and specificity of Matrix-Associated Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) in discrimination at species level for Acinetobacter bacteremia.

    abstract::We aimed to establish the role of MALDI-TOF MS on species discrimination of phenotypically indistinguishable A. baumannii, A. pittii and A. nosocomialis. Compared to multiplex PCR, the gold standard, MALDI-TOF MS yielded a high sensitivity for A. baumannii (97.9%) and specificity for A. pittii (98.9%) and A. nosocomia...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Wu MS,Collier S,Liu PY,Lee YT,Kuo SC,Yang YS,Chen TL,Shi ZY,Lin CF

    更新日期:2017-09-01 00:00:00

  • A quantitative analysis of DNA extraction and purification from compost.

    abstract::We quantified both DNA and humic acid concentrations during the extraction and purification of DNA from compost. The DNA extraction method consisted of bead-beating with SDS for cell lysis, poly(ethylene glycol)-8000 precipitation for preliminary DNA purification, and chromatography on a 10-ml Sephadex G-200 column fo...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Howeler M,Ghiorse WC,Walker LP

    更新日期:2003-07-01 00:00:00

  • Construction of a conditional lethal Salmonella mutant via genetic recombination using the ara system and asd gene.

    abstract::In order to construct a conditional lethal Salmonella mutant, an arabinose-regulated recombinant genetic system was used. The Salmonella aspartate semialdehyde dehydrogenase (asd) gene was localized under the control of araC P(araBAD) in a plasmid to create the araC P(araBAD)::asd cassette. The cassette was cloned int...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Kim SW,Kang HY,Hur J,Gal SW,Bang WY,Cho KK,Kim CW,Bahk JD,Lee JH

    更新日期:2011-11-01 00:00:00

  • Use of green fluorescent protein to quantify the growth of Colletotrichum during infection of tobacco.

    abstract::To develop a quantitative assay of fungal growth inside plant tissues, strains of Colletotrichum destructivum and Colletotrichum orbiculare were transformed with a modified green fluorescent protein (GFP) gene fused with a glyceraldehyde-3-phosphate dehydrogenase promoter from Aspergillus nidulans. Transformants expre...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Chen N,Hsiang T,Goodwin PH

    更新日期:2003-04-01 00:00:00

  • Use of Enterococcus faecalis and Bacillus atrophaeus as surrogates to establish and maintain laboratory proficiency for concentration of water samples using ultrafiltration.

    abstract::The U.S. Environmental Protection Agency's (EPA) Water Laboratory Alliance (WLA) currently uses ultrafiltration (UF) for concentration of biosafety level 3 (BSL-3) agents from large volumes (up to 100-L) of drinking water prior to analysis. Most UF procedures require comprehensive training and practice to achieve and ...

    journal_title:Journal of microbiological methods

    pub_type: 杂志文章


    authors: Mapp L,Klonicki P,Takundwa P,Hill VR,Schneeberger C,Knee J,Raynor M,Hwang N,Chambers Y,Miller K,Pope M

    更新日期:2015-11-01 00:00:00