Abstract:
:Rapid enumeration of Escherichia coli strains by quantitative real-time PCR targeting the uidA gene was developed and confirmed for minced beef, tuna and raw oyster. Higher sensitivity (1 CFU/g of E. coli in all three food samples) was obtained by incubating for 7 h in TSB. Colony-directed E. coli specific TaqMan PCR assay could effectively distinguish colonies grown on various selective media within 1.5-h. Inspection of E. coli in food testing laboratories is important, and our rapid E. coli detection strategy will contribute to quality control in food industries.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Takahashi H,Kimura B,Tanaka Y,Shinozaki J,Suda T,Fujii Tdoi
10.1016/j.mimet.2009.08.002subject
Has Abstractpub_date
2009-10-01 00:00:00pages
124-7issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(09)00237-1journal_volume
79pub_type
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