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Quantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR.

Abstract：

:Four different bacterial DNA extraction strategies and two different qPCR probe chemistries were studied for detection of Stapylococcus aureus from white cheeses. Method employing trypsin treatment followed by a commercial kit application and TaqMan probe-based qPCR was the most sensitive one detecting higher counts than standards in naturally contaminated samples.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Kadiroğlu P,Korel F,Ceylan C

doi

10.1016/j.mimet.2014.06.022

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

92-7

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(14)00185-7

journal_volume

105

pub_type

杂志文章

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