A tetracycline-inducible expression vector for Streptococcus agalactiae allowing controllable gene expression.

Abstract:

:Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that pG+off is characterized by tight repression under non-induced condition.

journal_name

J Microbiol Methods

authors

Lartigue MF,Bouloc P

doi

10.1016/j.mimet.2013.10.020

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

16-8

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(13)00335-7

journal_volume

96

pub_type

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