Abstract：
:The need for improved pathogen separation and concentration methods to reduce time-to-detection for foodborne pathogens is well recognized. Apolipoprotein H (ApoH) is an acute phase human plasma protein that has been previously shown to interact with viruses, lipopolysaccharides (LPS) and bacterial proteins. The purpose of this study was to determine if ApoH was capable of binding and efficiently capturing two representative human norovirus strains (GI.1 and GII.4), a cultivable surrogate, and four bacterial pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica serovar Enteritidis, and Staphylococcus aureus). Experiments were carried out using an ApoH-conjugated magnetic bead-based capture followed by pathogen detection using nucleic acid amplification. For all three viruses studied, >10% capture efficiency (<1 Log10 loss in RT-qPCR amplifiable units) was observed. The same capture efficiencies were observed for the bacterial pathogens tested, with the exception of E. coli O157:H7 (approximately 1% capture efficiency, or 2 Log10 loss in CFU equivalents). The efficiency of the capture steps did not vary as a consequence of input target concentration or in the presence of an abundance of background microflora. A complementary plate-based capture assay showed that ApoH bound to a variety of human norovirus virus-like particles. ApoH has the potential to be a broadly reactive ligand for separating and concentrating representative foodborne pathogens, both bacteria and viruses.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Almand EA,Goulter RM,Jaykus LAdoi
10.1016/j.mimet.2016.07.014subject
Has Abstractpub_date
2016-09-01 00:00:00pages
88-95eissn
0167-7012issn
1872-8359pii
S0167-7012(16)30186-5journal_volume
128pub_type
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journal_title：Journal of microbiological methods
pub_type： 杂志文章,评审
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
pub_type： 杂志文章
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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