Abstract：
:This paper reports a novel electrochemical immunosensor for the sensitive detection of staphylococcal enterotoxin A (SEA) based on self-assembly monolayer (SAM) and protein A immobilization on gold electrode. Three different methods of protein A immobilization were tested: physical adsorption, cross-linking using glutaraldehyde and covalent binding after activation with N-hydroxysuccinimide (NHS)/N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) on cysteamine-modified gold electrode. The EDC/NHS method for protein A immobilization was selected to lead development of the biosensor. The coating steps of the surface modification were characterized by cyclic voltammetry and the biosensor response by chronoamperometry. The advantages of the immunosensor were exposed in its high sensitivity and specificity. The proposed amperometric immunosensor was successfully used for determination of SEA in contaminated and non-contaminated cheese samples with excellent responses.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Pimenta-Martins MG,Furtado RF,Heneine LG,Dias RS,Borges Mde F,Alves CRdoi
10.1016/j.mimet.2012.05.016subject
Has Abstractpub_date
2012-10-01 00:00:00pages
138-43issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(12)00205-9journal_volume
91pub_type
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