Abstract：
:Representational Difference Analysis (RDA) is an established technique used for isolation of specific genetic differences between or within bacterial species. This method was used to investigate the genetic basis of serovar-specificity and the relationship between serovar and virulence in Haemophilus parasuis. An RDA clone library of 96 isolates was constructed using H. parasuis strains H425(P) (serovar 12) and HS1967 (serovar 4). To screen such a large clone library to determine which clones are strain-specific would typically involved separately labelling each clone for use in Southern hybridisation against genomic DNA from each of the strains. In this study, a novel application of reverse Southern hybridisation was used to screen the RDA library: genomic DNA from each strain was labelled and used to probe the library to identify strain-specific clones. This novel approach represents a significant improvement in methodology that is rapid and efficient.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Lancashire JF,Turni C,Blackall PJ,Jennings MPdoi
10.1016/j.mimet.2006.09.011subject
Has Abstractpub_date
2007-02-01 00:00:00pages
326-30issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(06)00276-4journal_volume
68pub_type
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