Abstract：
:Quantification of the biosurfactants produced by a variety of microorganisms is a time taking and difficult task due to the lack of rapid, efficient and accurate methods. This work presents a simple turbidometric method for quantification of crude biosurfactants based on their property to become insoluble at low pH values. Biosurfactants obtained from a Bacillus sp. using different carbon substrates showed a good linear correlation (R(2)>0.99) between biosurfactant concentrations and turbidity in the range of 1 to 10 g L(-1) of crude biosurfactants. The substrate specific equations (SSE) and generalized equations (GE) developed in this work effectively predicted the amount of crude biosurfactant produced in different sets of fermentation experiments validating the method. A similar linear correlation was also observed with biosurfactants obtained from two other strains, Bacillus circulans and Pseudomonas sp. This simple method may prove to be effective in fast, accurate and inexpensive quantification of crude biosurfactants produced by diverse bacteria.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Mukherjee S,Das P,Sen Rdoi
10.1016/j.mimet.2008.09.010subject
Has Abstractpub_date
2009-01-01 00:00:00pages
38-42issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(08)00340-0journal_volume
76pub_type
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