Abstract：
:Bacterial fruit blotch (BFB) of cucurbits, caused by Acidovorax citrulli, is a major threat to commercial watermelon and melon production worldwide. At present, there are at least two genetically distinct sub-populations (group I and II) of A. citrulli that differ in host preference among cucurbit species and copper sensitivity. In this study, we analyzed the pilA gene sequences of 103 A. citrulli strains from China and other countries. Based on these data, we classified all tested A. citrulli strains into three types. The pilA-based type 1 strains in this study coincided with the previously established group I strains; while the type 2 strains coincided with group II strains. Ten strains that did not cluster with group I or II strains were classified into a new type, designated type 3. Based on differences in pilA sequences, we designed a multiplex PCR assay to distinguish the three A. citrulli pilus types. This multiplex PCR assay has proven to be viable for strain typing of 139 A. citrulli strains and for the detection of this pathogen in artificially inoculated seeds and leaves and naturally infected leaves and fruits. This assay proved to be rapid, accurate, reliable and applicable for early distinction of A. citrulli types associated with BFB epidemics. It may also inform the judicious and environmentally sound use of bactericides, especially copper-based compounds.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Yang YW,Zhao M,Zhang LQ,Qiao P,Bai X,Zhang XX,Walcott RR,Guan W,Zhao TCdoi
10.1016/j.mimet.2019.02.003subject
Has Abstractpub_date
2019-03-01 00:00:00pages
93-98eissn
0167-7012issn
1872-8359pii
S0167-7012(18)30790-5journal_volume
158pub_type
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