当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > Spectrofluorimetric method for the estimation of total lipids in Eremothecium ashbyii fungal filaments using Nile blue and avoiding interference of autofluorescent riboflavin.

Spectrofluorimetric method for the estimation of total lipids in Eremothecium ashbyii fungal filaments using Nile blue and avoiding interference of autofluorescent riboflavin.

Abstract：

:A rapid, simple and sensitive spectrofluorimetric technique was developed for monitoring total lipids in hyphae of the riboflavin-overproducing fungus Eremothecium ashbyii using the fluorescent probe Nile blue in an aqueous system, avoiding the interference due to autofluorescent riboflavin. The existing methodologies for lipid estimation are tedious, requiring large biomass, solvent extraction and gravimetry. E. ashbyii is a hemiascomycete fungus which accumulates lipids in its mycelia prior to flavinogenesis. This study defines the conditions (wavelength selection and sensitivity) for the spectrofluorimetric quantification of lipids in situ in the macerated mycelia of this fungus in the presence of intracellular autofluorescent riboflavin without the need to extract the lipids from the mycelia. The fluorescent intensity was linear with the lipid concentration of the mycelia (by gravimetry) under three different growth conditions using glucose, olive oil and sunflower oil as carbon sources. This spectrofluorimetic method of lipid estimation can be applied to other fungi and microorganisms.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Vijayalakshmi S,Karthika TN,Mishra AK,Chandra TS

doi

10.1016/s0167-7012(03)00137-4

subject

Has Abstract

pub_date

2003-10-01 00:00:00

pages

99-103

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167701203001374

journal_volume

55

pub_type

杂志文章

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