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Tryptic soy medium is feasible for the in situ preparation of standards containing small defined numbers of microbial cells.

Abstract：

:A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strains.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Matsuoka H,Shigetomi T,Funabashi H,Saito M,Igimi S

doi

10.1016/j.mimet.2013.01.021

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

49-51

issue

1

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(13)00043-2

journal_volume

93

pub_type

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