当前位置： SCI文献检索 > JOURNAL OF MICROBIOLOGICAL METHODS期刊下所有文献 > A novel 96-well gel-based assay for determining antifungal activity against filamentous fungi.

A novel 96-well gel-based assay for determining antifungal activity against filamentous fungi.

Abstract：

:In recent years the global rise in antibiotic resistance and environmental consciousness lead to a renewed fervour to find and develop novel antibiotics, including antifungals. However, the influence of the environment on antifungal activity is often disregarded and many in vitro assays may cause the activity of certain antifungals to be overestimated or underestimated. The general antifungal test assays that are economically accessible to the majority of scientists primarily rely on visual examination or on spectrophotometric analysis. The effect of certain morphogenic antifungals, which may lead to hyperbranching of filamentous fungi, unfortunately renders these methods unreliable. To minimise the difficulties experienced as a result of hyperbranching, we developed a straightforward, economical 96-well gel-based method, independent of spectrophotometric analysis, for highly repeatable determination of antifungal activity. For the calculation of inhibition parameters, this method relies on the visualisation of assay results by digitisation. The antifungal activity results from our novel micro-gel dilution assay are comparable to that of the micro-broth dilution assay used as standard reference test of The Clinical and Laboratory Standard Institute. Furthermore, our economical assay is multifunctional as it permits microscopic analysis of the preserved assay results, as well as rendering highly reliable data.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Troskie AM,Vlok NM,Rautenbach M

doi

10.1016/j.mimet.2012.09.025

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

551-8

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(12)00313-2

journal_volume

91

pub_type

杂志文章

相关文献

JOURNAL OF MICROBIOLOGICAL METHODS文献大全
  • An improved high throughput sequencing method for studying oomycete communities.

    abstract：:Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.01.013

    authors： Sapkota R,Nicolaisen M

    更新日期：2015-03-01 00:00:00

  • Comparative assessment of next-generation sequencing, denaturing gradient gel electrophoresis, clonal restriction fragment length polymorphism and cloning-sequencing as methods for characterizing commercial microbial consortia.

    abstract：:Characterization of commercial microbial consortia products for human and environmental health risk assessment is a major challenge for regulatory agencies. As a means to develop an approach to assess the potential environmental risk of these products, research was conducted to compare four genomics methods for charac...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.11.013

    authors： Samarajeewa AD,Hammad A,Masson L,Khan IU,Scroggins R,Beaudette LA

    更新日期：2015-01-01 00:00:00

  • Facile construction of unmarked deletion mutants in Burkholderia pseudomallei using sacB counter-selection in sucrose-resistant and sucrose-sensitive isolates.

    abstract：:Burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic or emerging world-wide. Here we report unmarked allele-replacement mutagenesis using efficient sacB counter-selection. Despite being genotypically sacB(+), most commonly used B. pseudomallei strains are sucrose-resista...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2008.12.007

    authors： Logue CA,Peak IR,Beacham IR

    更新日期：2009-03-01 00:00:00

  • A simple, rapid, sensitive method detecting homoserine lactone (HSL)-related compounds in microbial extracts.

    abstract：:A simple, rapid, sensitive microtiter plate method detecting N-acyl homoserine lactone (HSL)-related compounds was established using an Agrobacterium tumefaciens strain harboring a traG::lacZ/traR reporter gene responsive to HSLs. This strain did not produce its own HSL, but the traG::lacZ reporter gene was induced on...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.06.003

    authors： Singh MP,Greenstein M

    更新日期：2006-04-01 00:00:00

  • Improved detection of microbial DNA after bead-beating before DNA isolation.

    abstract：:Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbia...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.11.009

    authors： de Boer R,Peters R,Gierveld S,Schuurman T,Kooistra-Smid M,Savelkoul P

    更新日期：2010-02-01 00:00:00

  • Towards a unified system for detecting waterborne pathogens.

    abstract：:Currently, there is no single method to collect, process, and analyze a water sample for all pathogenic microorganisms of interest. Some of the difficulties in developing a universal method include the physical differences between the major pathogen groups (viruses, bacteria, protozoa), efficiently concentrating large...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章,评审

    doi：10.1016/s0167-7012(03)00023-x

    authors： Straub TM,Chandler DP

    更新日期：2003-05-01 00:00:00

  • Suitability of genomic DNA synthesized by strand displacement amplification (SDA) for AFLP analysis: genotyping single spores of arbuscular mycorrhizal (AM) fungi.

    abstract：:Limited biological samples of microbial origin often yield insufficient amounts of genomic DNA, making application of standard techniques of genetic analysis, like amplified fragment length polymorphism (AFLP), virtually impossible. The Phi29 DNA polymerase based whole genome amplification (WGA) method has the potenti...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2005.03.009

    authors： Gadkar V,Rillig MC

    更新日期：2005-11-01 00:00:00

  • tuf-PCR-temporal temperature gradient gel electrophoresis for molecular detection and identification of staphylococci: application to breast milk and neonate gut microbiota.

    abstract：:Coagulase negative staphylococci (CoNS) are a leading cause of infections in preterm infants, mostly involved in late-onset infection in low birth weight neonates. The epidemiology and pathophysiology of these infections remain unclear, notably because the causing agents are gathered in the artificial CoNS group. The ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.12.022

    authors： Filleron A,Simon M,Hantova S,Jacquot A,Cambonie G,Marchandin H,Jumas-Bilak E

    更新日期：2014-03-01 00:00:00

  • MALDI-TOF MS based identification of food-borne yeast isolates.

    abstract：:In this study, food-borne yeast isolates (n=96), comprising at least 33 species, were identified using MALDI-TOF MS and conventional methods (API ID 32 C and Phoenix Yeast ID). Discrepancies of both methods were resolved by sequencing the ITS1-5.8S-rRNA-ITS2 region. For ten isolates, mainly classified to Rhodotorula a...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.08.021

    authors： Pavlovic M,Mewes A,Maggipinto M,Schmidt W,Messelhäußer U,Balsliemke J,Hörmansdorfer S,Busch U,Huber I

    更新日期：2014-11-01 00:00:00

  • High resolution clear native electrophoresis is a good alternative to blue native electrophoresis for the characterization of the Escherichia coli membrane complexes.

    abstract：:Blue native electrophoresis (BNE) has become the most popular method for the global analysis of membrane protein complexes. Although it has been shown to be very useful for that purpose, it can produce the dissociation of complexes with weak interactions and, due to the use of Coomassie Brilliant Blue, does not allow ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.05.003

    authors： Diéguez-Casal E,Freixeiro P,Costoya L,Criado MT,Ferreirós C,Sánchez S

    更新日期：2014-07-01 00:00:00

  • Comparative performance of Aspergillus galactomannan ELISA and PCR in sputum from patients with ABPA and CPA.

    abstract：OBJECTIVES:Galactomannan (GM) and Aspergillus DNA detection are useful tools for the diagnosis of invasive pulmonary aspergillosis (IPA), primarily in blood and bronchoscopy samples. This study aimed to evaluate the utility of both markers for detection of Aspergillus in sputum from patients with allergic bronchopulmon...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.06.016

    authors： Fayemiwo S,Moore CB,Foden P,Denning DW,Richardson MD

    更新日期：2017-09-01 00:00:00

  • Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers.

    abstract：:A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.09.002

    authors： Santos CS,Ramos JN,Vieira VV,Pinheiro CS,Meyer R,Alcantara-Neves NM,Ramos RT,Silva A,Hirata R Jr,Felicori L,de Alegría Puig CR,Navas J,Azevedo V,Mattos-Guaraldi AL,Pacheco LGC

    更新日期：2017-11-01 00:00:00

  • Rapid detection of Haemophilus parasuis using cross-priming amplification and vertical flow visualization.

    abstract：:Haemophilus parasuis infection is of considerable economic importance in the swine industry due to high morbidity and mortality in naive swine populations. Accurate detection and identification of the causative agent are difficult, yet necessary, for disease control. In this study, a simple and rapid method of cross-p...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2017.11.005

    authors： Gou H,Li J,Cai R,Song S,Li M,Yang D,Jiang Z,Li Y,Chu P,Li C

    更新日期：2018-01-01 00:00:00

  • Rapid species and strain differentiation of non-tubercoulous mycobacteria by Fourier-Transform Infrared microspectroscopy.

    abstract：:Rapid identification of non-tuberculous mycobacteria (NTM) species is important in clinical laboratories to stipulate the appropriate therapy and to offer a comprehensive infection control. We applied Fourier-Transform Infrared microspectroscopy to evaluate, whether the most frequent species of NTM can be rapidly and ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2006.08.011

    authors： Rebuffo-Scheer CA,Kirschner C,Staemmler M,Naumann D

    更新日期：2007-02-01 00:00:00

  • Screening for peptides binding on Phytophthora capsici extracts by phage display.

    abstract：:Phytophthora blight of pepper is a completely destructive plant disease caused by the oomycete pathogen Phytophthora capsici Leonian. Phytophthora disease is responsible for major losses in pepper production and the pathogen can survive in soil in the absence of the host plant for many years. Currently, there are no e...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.04.006

    authors： Park HY,Park HC,Yoon MY

    更新日期：2009-07-01 00:00:00

  • A strategy to verify the absence of the pgm locus in Yersinia pestis strain candidates for select agent exemption.

    abstract：:Yersinia pestis is a Department of Health and Human Services select agent as defined in federal regulations. Certain attenuated strains of Y. pestis, such as the pgm(-) strain, are exempt from these regulations. Herein we describe a strategy to verify the absence of the pgm locus in Y. pestis strains being considered ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.02.013

    authors： Jenkins AL,Worsham PL,Welkos SL

    更新日期：2009-06-01 00:00:00

  • Development and validation of a droplet digital PCR assay for the detection and quantification of Bartonella species within human clinical samples.

    abstract：:This report describes the development, optimization, and validation of a ddPCR assay for the detection of Bartonella spp. DNA within several sample matrices, including clinical blood samples from patients with or without documented Bartonella spp. bacteremia. The Bartonella spp. ddPCR assay was developed based upon pr...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2020.106022

    authors： Maggi RG,Richardson T,Breitschwerdt EB,Miller JC

    更新日期：2020-09-01 00:00:00

  • Immunoglobulin cleavage by the streptococcal cysteine protease IdeS can be detected using protein G capture and mass spectrometry.

    abstract：:The immunoglobulin degrading enzyme of Streptococcus pyogenes, IdeS, is an unusual cysteine protease produced by group A streptococci for which the only known substrate is immunoglobulin G (IgG). To date, IdeS has not been found to cleave any of the known synthetic substrates that other cysteine proteases hydrolyse, t...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.04.017

    authors： Hess JL,Porsch EA,Shertz CA,Boyle MD

    更新日期：2007-08-01 00:00:00

  • Simplified amplified-fragment length polymorphism method for genotyping Mycobacterium tuberculosis isolates.

    abstract：:A simplified amplified-fragment length polymorphism (AFLP) method was developed and applied to genotype 52 Mycobacterium tuberculosis isolates. This method can be carried out using only one restriction enzyme (XhoI), one double strand adapter, and one PCR primer. The amounts of DNA and DNA polymerase, and the concentr...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2009.07.007

    authors： Viader-Salvadó JM,Flores-Gracia J,Vega-Alonso AS,Treviño-Alvarado VM,Molina-Torres CA,Vera-Cabrera L,Guerrero-Olazarán M

    更新日期：2009-09-01 00:00:00

  • Evaluation of Ag containing hydroxyapatite coatings to the Candida albicans infection.

    abstract：:In this research work, the synthesis of Ag doped hydroxyapatite coatings for dental or orthopedic implants was performed. The main goal was to determine the influence of Ag content on the roughness and antimicrobial performance of the prepared thin films. The films were deposited on Ti6Al4V alloy by means of RF magnet...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2016.03.016

    authors： Ciuca S,Badea M,Pozna E,Pana I,Kiss A,Floroian L,Semenescu A,Cotrut CM,Moga M,Vladescu A

    更新日期：2016-06-01 00:00:00

  • The use of a rapid assay to detect the neuraminidase production in oral Porphyromonas spp. isolated from dogs and humans.

    abstract：:Neuraminidase was produced by 32.1% and 28.5% of Porphyromonas from dogs with and without periodontitis, respectively; and by 31.8% of bacteria from humans. The presence of neuraminidase in Porphyromonas spp. suggests that this enzyme can be involved with the pathogenesis of the periodontal disease, and the use of thi...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2013.06.017

    authors： de Assis PR,Nakano V,Senhorinho GN,Avila-Campos MJ

    更新日期：2013-09-01 00:00:00

  • Relating growth dynamics and glucoamylase excretion of individual Saccharomyces cerevisiae cells.

    abstract：:We have developed a novel flow cytometric procedure that allows determinations of properties of protein excretion in the growth medium on a cell-by-cell basis in Saccharomyces cerevisiae. The procedure is based on labelling of a periplasmically secreted protein with antibodies conjugated to a fluorescent marker such a...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/s0167-7012(00)00171-8

    authors： Porro D,Venturini M,Brambilla L,Alberghina L,Vanoni M

    更新日期：2000-09-01 00:00:00

  • WITHDRAWN: Multiplex particle refining system to enhance Cryptosporidium recovery for surface water filtration methods.

    abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, http://dx.org/doi:10.1016/j.mimet.2012.12.009. The duplicate article has therefore been withdrawn. ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2012.12.009

    authors： Pires NM,Dong T

    更新日期：2012-12-20 00:00:00

  • Identification and differentiation of food-related bacteria: A comparison of FTIR spectroscopy and MALDI-TOF mass spectrometry.

    abstract：:The food industry requires easy, accurate, and cost-effective techniques for microbial identification to ensure safe products and identify microbial contaminations. In this work, FTIR spectroscopy and MALDI-TOF mass spectrometry were assessed for their suitability and applicability for routine microbial diagnostics of...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.05.011

    authors： Wenning M,Breitenwieser F,Konrad R,Huber I,Busch U,Scherer S

    更新日期：2014-08-01 00:00:00

  • A PCR-based method to quantify fungal growth during pretreatment of lignocellulosic biomass.

    abstract：:Filamentous fungi have shown great potential in the pretreatment of lignocellulosic biomass and their use in bio-processes based on Solid State Fermentation (SSF) opens promising perspectives for plant biomass valorization. Obviously, quantification of the fungal biomass throughout the fermentation is a crucial parame...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2015.05.024

    authors： Simeng Z,Sacha G,Isabelle HG,Marie-Noëlle R

    更新日期：2015-08-01 00:00:00

  • Development of a novel plasmid vector pTIO-1 adapted for electrotransformation of Porphyromonas gingivalis.

    abstract：:We report here the construction of a plasmid vector designed for the efficient electrotransformation of the periodontal pathogen Porphyromonas gingivalis. The novel Escherichia coli-Bacteroides/P. gingivalis shuttle vector, designated pTIO-1, is based on the 11.0-kb E. coli-Bacteroides conjugative shuttle vector, pVAL...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.07.032

    authors： Tagawa J,Inoue T,Naito M,Sato K,Kuwahara T,Nakayama M,Nakayama K,Yamashiro T,Ohara N

    更新日期：2014-10-01 00:00:00

  • Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes.

    abstract：:Two rapid in situ enumeration methods using fluorescent probes were used to assess the physiological activities of Klebsiella pneumoniae biofilms on stainless steel. Fluorescent dyes, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and rhodamine 123 (Rh 123), were chosen to perform this study. CTC is a soluble redox ...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/0167-7012(94)90058-2

    authors： Yu FP,McFeters GA

    更新日期：1994-01-01 00:00:00

  • Comparative genomics study for the identification of drug and vaccine targets in Staphylococcus aureus: MurA ligase enzyme as a proposed candidate.

    abstract：:Now-a-days increasing emergence of antibiotic-resistant pathogenic microorganisms is one of the biggest challenges for management of disease. In the present study comparative genomics, metabolic pathways analysis and additional parameters were defined for the identification of 94 non-homologous essential proteins in S...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.03.009

    authors： Ghosh S,Prava J,Samal HB,Suar M,Mahapatra RK

    更新日期：2014-06-01 00:00:00

  • Antigenicity and immunogenicity of fused B-subunit of heat labile toxin of Escherichia coli and colonization factor antigen I polyepitopes.

    abstract：:Linear B-cell epitopes ((93)AKEFEAAAL(101) and (66)PQLTDVLN(73)) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2014.07.035

    authors： Savar NS,Dashti A,Darzi Eslam E,Jahanian-Najafabadi A,Jafari A

    更新日期：2014-11-01 00:00:00

  • Efficient recovery of low endotoxin medium-chain-length poly([R]-3-hydroxyalkanoate) from bacterial biomass.

    abstract：:Bacterial polyhydroxyalkanoate (PHA) is an attractive biopolyester for medical applications due to its biocompatibility. However, inappropriate extraction of PHA from bacterial biomass results in contamination by pyrogenic compounds (e.g. lipopolysaccharides) and thus influences medical testing. This problem was solve...

    journal_title：Journal of microbiological methods

    pub_type： 杂志文章

    doi：10.1016/j.mimet.2007.01.002

    authors： Furrer P,Panke S,Zinn M

    更新日期：2007-04-01 00:00:00