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Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR.

Abstract：

:A PMA (propidium monoazide) pretreatment protocol, in which PMA is applied directly to membrane filters, was developed for the PCR-based quantification (PMA-qPCR) of viable Legionella pneumophila. Using this method, the amplification of DNA from membrane-damaged L. pneumophila was strongly inhibited for samples containing a small number of dead bacteria.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Slimani S,Robyns A,Jarraud S,Molmeret M,Dusserre E,Mazure C,Facon JP,Lina G,Etienne J,Ginevra C

doi

10.1016/j.mimet.2011.12.010

subject

Has Abstract

pub_date

2012-02-01 00:00:00

pages

319-21

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(11)00436-2

journal_volume

88

pub_type

杂志文章

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