Abstract:
:Confocal Raman Microspectroscopy (CRM) can be used as a tool for the in situ evaluation of the chemical composition of living, fully submerged, unstained biofilms. In this study the estimation of the local water content in Pseudomonas aeruginosa PA01 biofilms is given as an example. The ratio of the area of the O-H stretching vibration band at 3450 cm(-1), (water), to that of the C-H stretching bands at 2950 cm(-1) (biomass), was used to estimate the relative biofilm water content. The quantification of biofilm water and biomass was based on calibration curves generated from protein solutions. Water/biomass ratios (W:BR) equivalent to that of a 30% (w/v) protein solution were observed within some biofilm colonies.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Sandt C,Smith Palmer T,Pink J,Pink Ddoi
10.1016/j.mimet.2008.05.012subject
Has Abstractpub_date
2008-09-01 00:00:00pages
148-52issue
1eissn
0167-7012issn
1872-8359pii
S0167-7012(08)00157-7journal_volume
75pub_type
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