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Simultaneous detection of six human diarrheal pathogens by using DNA microarray combined with tyramide signal amplification.

Abstract：

:Multiplex PCR and DNA microarray were combined with tyramide signal amplification (TSA) to develop a reliable method suitable for simultaneous detection of six species of human diarrheal pathogens (Yersinia enterocolitica, Shigella spp, Salmonella typhi, Brucella spp, Vibrio cholera and Escherichia coli O157:H7). Meanwhile, our method could distinguish V. cholera serotype O1 from O139, and O157:H7 from O157: non-H7. This assay conferred a specificity of 100% for target pathogens. The limit of detection was 103 degrees CFU/mL approximately. The results of 98.6% (357/362) clinical specimens and 100% (5/5) mocked double-blind samples were the same to that from conventional assay. Consequently this assay is sensitive and a specific tool suitable for diagnostic detection and surveillance of multiple human pathogens.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Jin D,Qi H,Chen S,Zeng T,Liu Q,Wang S

doi

10.1016/j.mimet.2008.06.020

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

365-8

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(08)00245-5

journal_volume

75

pub_type

杂志文章

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