Abstract：
:The purpose of this study was to develop a detection method for viable E. coli O157:H7 in fresh produce and recreational water. The method was evaluated using eight samples of produce wash and recreational water with or without spiked E. coli O157:H7 at ≤10(2) CFU·ml(-1) and concentrated using dead-end ultrafiltration (DEUF) to produce primary and secondary retentates. Fifty-four matrix replicates of undiluted secondary retentates or dilutions (1:2 or 1:10 in buffer) were evaluated using an IMS/ATP bioluminescence assay (IMS/ATP). Combining primary and secondary DEUF yielded a 2-4 log(10) increase in E. coli O157:H7 concentrations in spiked samples and resulted in signal-to-noise ratios 2-219 fold higher than controls, depending on the sample type. DEUF increased the concentration of E. coli O157:H7 to within the detectable limits of IMS/ATP. The combined assay provided detection of viable E. coli O157:H7 in produce and recreational water. Accurate detection of microbial pathogens using DEUF and IMS/ATP could reduce disease outbreaks from contaminated water sources and food products.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Hunter DM,Leskinen SD,Magaña S,Schlemmer SM,Lim DVdoi
10.1016/j.mimet.2011.09.010subject
Has Abstractpub_date
2011-12-01 00:00:00pages
338-42issue
3eissn
0167-7012issn
1872-8359pii
S0167-7012(11)00329-0journal_volume
87pub_type
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