Abstract：
:We modified Wayne's pyrazinamidase test against Mycobacterium tuberculosis to indirectly measure pyrazinamidase activity via pyrazinoic acid in liquid medium. The modified pyrazinamidase test was easy to perform and its results were in complete agreement with those of the conventional Wayne's method, highlighting its potential application in phenotypic pyrazinamide susceptibility testing.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Aono A,Chikamatsu K,Yamada H,Igarashi Y,Murase Y,Takaki A,Mitarai Sdoi
10.1016/j.mimet.2018.09.018subject
Has Abstractpub_date
2018-11-01 00:00:00pages
52-54eissn
0167-7012issn
1872-8359pii
S0167-7012(18)30454-8journal_volume
154pub_type
杂志文章abstract：:Acidobacteriota are highly abundant in soils, however, few cultured representatives are available. The purity of the reagents can influence microbial growth in laboratory conditions and successful isolation. Here we investigated the impact of different agar brands in culture medium and advocate that agar origin should...
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journal_title：Journal of microbiological methods
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abstract：:The purpose of this study was to determine the interactions between arsenic (As) resistant bacteria and As, using surface-enhanced Raman scattering (SERS) and Fourier transform infrared (FTIR) spectroscopy. According to our 16S rDNA results, eight bacteria isolated from the environment can be identified to four genera...
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journal_title：Journal of microbiological methods
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abstract：:Xylella fastidiosa is an insect-transmitted bacterial plant pathogen which causes a variety of economically important diseases worldwide. Molecular identification of X. fastidiosa is used for quarantine screening, surveillance, and research applications; many of which require subspecies level differentiation of pathog...
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journal_title：Journal of microbiological methods
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doi：10.1016/s0167-7012(02)00106-9
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abstract：:Small-colony variants (SCVs) are slow-growing subpopulations of various auxotrophic bacterial strains. Thymidine-dependent SCVs (TD-SCVs) are unable to synthesize thymidine; hence, these variants fail to grow in a medium without thymidine. In this study, we used 10 TD-SCVs of Staphylococcus aureus, of which four strai...
journal_title：Journal of microbiological methods
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doi：10.1016/j.mimet.2015.01.007
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doi：10.1016/j.mimet.2011.06.005
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abstract：:A temperature-gradient incubator (TGI) is described, which produces a thermal gradient over 34 aluminium modules (15x30x5 cm) intersected by 2-mm layers of partly insulating graphite foil (SigraFlex Universal). The new, sandwich-designed TGI has 30 rows of six replicate sample wells for incubation of 28-ml test tubes....
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journal_title：Journal of microbiological methods
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doi：10.1016/s0167-7012(99)00043-3
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journal_title：Journal of microbiological methods
pub_type： 杂志文章
doi：10.1016/j.mimet.2006.09.012
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journal_title：Journal of microbiological methods
pub_type： 杂志文章
doi：10.1016/j.mimet.2010.04.006
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journal_title：Journal of microbiological methods
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journal_title：Journal of microbiological methods
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doi：10.1016/j.mimet.2010.08.012
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abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, http://dx.org/doi:10.1016/j.mimet.2012.12.009. The duplicate article has therefore been withdrawn. ...
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