Abstract:
:Complementation of chromosomal mutations in trans can introduce artifacts due to the number of episomal copies of the gene in question. One solution is to study the gene expressed at a single ectopic site in cis. We have designed and constructed a vector that allows homologous recombination into a gene encoding a frame-shifted IS1016-V6 protein in the Haemophilus influenzae Rd KW20 chromosome (HI1018). This site is the location of the > or = 35 kilobase capsule locus in encapsulated type b and d strains. This locus is not present in the nontypeable Rd KW20 strain, thus allowing ectopic expression of genes homologously recombined into HI1018 without polar effects.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Daines DA,Smith ALdoi
10.1016/j.mimet.2004.02.014subject
Has Abstractpub_date
2004-06-01 00:00:00pages
421-4issue
3eissn
0167-7012issn
1872-8359pii
S0167701204000570journal_volume
57pub_type
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