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Attenuated colicin-based screening to discover and create novel resistance genes.

Abstract：

:I formulated a systematic approach to screen for colicin resistance-associated genes by using an attenuated colicin mutant and demonstrated its utility in a screen of genes related to colicin E5 resistance. Screening of an Escherichia coli genome library revealed rstA as a partial resistance gene to colicin E5. Transcript expression of BtuB and OmpF, proteins responsible for translocation of nuclease E colicins, was clearly inhibited in an rstA-overexpressing strain. In addition, the tatA::recN fusion gene provides resistance to an attenuated colicin E5 mutant. Improving tatA::recN by directed coevolution, I created a novel gene with enhanced resistance to colicin E5 and concluded that attenuated colicin-based screening is useful for the discovery and creation of novel colicin resistance-associated genes.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Futai K

doi

10.1016/j.mimet.2014.03.003

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

128-36

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(14)00075-X

journal_volume

100

pub_type

杂志文章

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