Abstract：
:Paper-based nucleic acid testing techniques are increasingly in demand. Hence, we have developed a simple and cheap paper fluidic device to detect multiple gene targets in Leptospira. Fluidic channels of the penta-clover device are drawn using a correction pen on Whatman filter paper 1. The fluid blocks the pores of the paper, avoiding leakage and ensuring the equal flow of sample to the reaction pads. The target genes are amplified by performing Loop-Mediated Isothermal Amplification (LAMP) with dry reaction components. Thecolor change of leuco crystal violetallows real-time monitoring of a positive amplification. The difference in color intensity is captured with a smartphone and analyzed using image processing software. The device amplifies the target within 15 min, detects the pathogen at a concentration as low as 50 attogram μL-1, detects Leptospira in blood samples without prior treatment and differentiates the Leptospira species even after 21 days of storage at room temperature.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Varsha V,Aishwarya S,Murchana S,Naveen G,Ramya M,Rathinasabapathi Pdoi
10.1016/j.mimet.2020.105962subject
Has Abstractpub_date
2020-07-01 00:00:00pages
105962eissn
0167-7012issn
1872-8359pii
S0167-7012(19)30880-2journal_volume
174pub_type
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