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Colloidal chitin stained with Remazol Brilliant Blue R, a useful substrate to select chitinolytic microorganisms and to evaluate chitinases.

Abstract：

:A simple and sensitive method based on the use of colloidal chitin stained with Remazol Brilliant Blue R (RBB) is proposed to evaluate chitinase activity. If this colloidal-stained substrate is included as a carbon source in a liquid medium, this technique allows the selection or the comparison of chitinolytic microorganisms. The colloidal substrate is proportionally solubilized and the dye released is spectrophotometrically quantified at 595 nm. The procedures used for the staining and fixing of RBB in the colloidal chitin, and a comparison with the commercial substrate chitin-azure, are presented. The influence of several physicochemical and enzymatic parameters on the release of dyes is also shown. Both stained substrates were used for studying the effect of pH, substrate concentration, temperature and time on the chitinase reaction of Bacillus thuringiensis Bt-107.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Gómez Ramírez M,Rojas Avelizapa LI,Rojas Avelizapa NG,Cruz Camarillo R

doi

10.1016/j.mimet.2003.10.011

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

213-9

issue

2

eissn

0167-7012

issn

1872-8359

pii

S0167701203003014

journal_volume

56

pub_type

杂志文章

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