Abstract:
:Traditional culture and biochemical tests (CBT) were compared with PCR for sensitivity and detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in seeded faeces and clinical samples from diarrhoeic pigs. A duplex PCR system was developed based on primers detecting the tlyA-gene of B. hyodysenteriae and the 16S rRNA-gene of B. pilosicoli. Sensitivities for the PCR system were determined on seeded faeces, using DNA that had been recovered from primary cultures or extracted directly from faeces. Compared to CBT, PCR applied to DNA extracted directly from faeces lowered the sensitivity by a factor of 1000 to 10,000. B. hyodysenteriae and B. pilosicoli detection was compared for CBT and PCR using 200 clinical samples. CBT detected more B. hyodysenteriae isolates in the clinical samples than PCR, but fewer B. pilosicoli positive samples. An atypical strongly haemolytic isolate was detected only by CBT.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Råsbäck T,Fellström C,Gunnarsson A,Aspán Adoi
10.1016/j.mimet.2005.12.008subject
Has Abstractpub_date
2006-08-01 00:00:00pages
347-53issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(05)00387-8journal_volume
66pub_type
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