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A quick and accurate screening method for fungal gene-deletion mutants by direct, priority-based, and inverse PCRs.

Abstract：

:Using two-step-PCR screening which consists of direct and priority-based PCR and inverse PCR, fungal gene-deletion mutants were selected quickly and accurately. It omits genomic DNA extraction and Southern blotting steps and prevents misinterpretations caused by PCR failure. It is anticipated to facilitate large-scale reverse genetic studies in fungi.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Park J,Kim S,Kwon S,Lee YH

doi

10.1016/j.mimet.2014.06.023

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

39-41

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(14)00197-3

journal_volume

105

pub_type

杂志文章

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