Abstract:
:Now-a-days increasing emergence of antibiotic-resistant pathogenic microorganisms is one of the biggest challenges for management of disease. In the present study comparative genomics, metabolic pathways analysis and additional parameters were defined for the identification of 94 non-homologous essential proteins in Staphylococcus aureus genome. Further study prioritized 19 proteins as vaccine candidates where as druggability study reports 34 proteins suitable as drug targets. Enzymes from peptidoglycan biosynthesis, folate biosynthesis were identified as candidates for drug development. Furthermore, bacterial secretory proteins and few hypothetical proteins identified in our analysis fulfill the criteria of vaccine candidates. As a case study, we built a homology model of one of the potential drug target, MurA ligase, using MODELLER (9v12) software. The model has been further selected for in silico docking study with inhibitors from the DrugBank database. Results from this study could facilitate selection of proteins for entry into drug design and vaccine production pipelines.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Ghosh S,Prava J,Samal HB,Suar M,Mahapatra RKdoi
10.1016/j.mimet.2014.03.009subject
Has Abstractpub_date
2014-06-01 00:00:00pages
1-8eissn
0167-7012issn
1872-8359pii
S0167-7012(14)00081-5journal_volume
101pub_type
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