Abstract:
:The development of biofilms in static plates was monitored. Glass coupons were placed on agar covered with filter paper, which was inoculated with suspended bacteria. The viable cell density, biofilms matrix and biomass were quantified. The method is excellent for adhesion and material studies, due to its simplicity and flexibility.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Oja T,Blomqvist B,Buckingham-Meyer K,Goeres D,Vuorela P,Fallarero Adoi
10.1016/j.mimet.2014.10.008subject
Has Abstractpub_date
2014-12-01 00:00:00pages
157-60eissn
0167-7012issn
1872-8359pii
S0167-7012(14)00298-Xjournal_volume
107pub_type
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abstract::Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The ...
journal_title:Journal of microbiological methods
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abstract::A microplate-based rapid, inexpensive and robust technique is developed by using tetrazolium salt 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and menadione to determine the viability of Mycobacterium tuberculosis, Mycobacterium bovis BCG and Mycobacterium smegmatis bacilli in microp...
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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abstract::A new, simple approach for the isolation and quantitative analysis of the cell wall (CW) proteins from invasively growing Saccharomyces cerevisiae strains is described in this contribution. The proposed method was proved compatible with agar-invasion assays and was demonstrated to be useful as a screening tool for rap...
journal_title:Journal of microbiological methods
pub_type: 杂志文章
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journal_title:Journal of microbiological methods
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abstract::Corynebacterium matruchotii is a microbial inhabitant in the oral cavity of humans and is associated with the formation of dental calculi. C. matruchotii forms highly specific morphological units, which are referred to as corn-cobs. Although other Corynebacterium species have frequently been isolated from the oral cav...
journal_title:Journal of microbiological methods
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doi:10.1016/j.mimet.2014.06.005
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abstract::A method for rapidly and selectively isolating Salmonellae from buffer solutions and concentrating the bacteria by a factor of approximately 500 was developed. Anti-Salmonellae antibody was covalently linked to 40 microm polyacrylamide beads to prepare a solid phase with affinity for the bacteria. The beads were packe...
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
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abstract::Mycobacterium tuberculosis (MTB) is the causative agent of pulmonary tuberculosis. Rapid and accurate diagnosis is crucial to tuberculosis control and prevention. A series of diagnostic methods has been available for MTB detection; however, new rapid, simple and affordable methods are needed. In this study, a multiple...
journal_title:Journal of microbiological methods
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abstract::A simple, rapid, sensitive microtiter plate method detecting N-acyl homoserine lactone (HSL)-related compounds was established using an Agrobacterium tumefaciens strain harboring a traG::lacZ/traR reporter gene responsive to HSLs. This strain did not produce its own HSL, but the traG::lacZ reporter gene was induced on...
journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
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abstract::A 16S rRNA gene-based fingerprinting method was developed for the identification of Azotobacteraceae and tested onto 48 soil isolates and 28 reference strains belonging to the free-living nitrogen-fixing bacterial group and to the most common species found in soil samples. According to this method, the 16S rRNA gene w...
journal_title:Journal of microbiological methods
pub_type: 杂志文章
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journal_title:Journal of microbiological methods
pub_type: 杂志文章
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