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Establishment and validation of a 384-well antibacterial assay amenable for high-throughput screening and combination testing.

Abstract：

:A 384-well-based antibacterial assay amenable for high-throughput screening and combination testing is described. The assay uses 100-500nL of test compounds and tolerates up to 2.5% dimethyl sulfoxide concentrations. It can be used for screening compound libraries and testing combinatory/synergistic/antagonistic effects of antibiotics, small molecules, and natural product extracts.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Mishra A,Dobritsa SV,Crouch ML,Rabenstein J,Lee JX,Dhakshinamoorthy S

doi

10.1016/j.mimet.2015.09.019

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

173-5

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(15)30075-0

journal_volume

118

pub_type

杂志文章

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