Abstract：
:We developed an immunocapture-based polymerase chain reaction (PCR) assay for simultaneous detection of Cryptosporidium parvum oocysts and Giardia intestinalis cysts in surface water. Using primer pairs Cry9/Cry15 and LaxA/LaxB for Cryptosporidium and Gdh1/Gdh4 for Giardia, the sensitivity of the entire detection procedure (dealing with concentration, separation, DNA purification and PCR amplification) was at the level of 50-100 oocysts and cysts. Of 54 surface water samples, 4 were positive for Cryptosporidium and 1 for Giardia. Cryptosporidium and Giardia were detected for the first time in surface water in Finland.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Rimhanen-Finne R,Hörman A,Ronkainen P,Hänninen MLdoi
10.1016/s0167-7012(02)00047-7subject
Has Abstractpub_date
2002-08-01 00:00:00pages
299-303issue
3eissn
0167-7012issn
1872-8359pii
S0167701202000477journal_volume
50pub_type
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