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Efficient sequential repetitive gene deletions in Neurospora crassa employing a self-excising β-recombinase/six cassette.

Abstract：

:Despite its long-standing history as a model organism, Neurospora crassa has limited tools for repetitive gene deletions utilizing recyclable self-excising marker systems. Here we describe, for the first time, the functionality of a bacterial recombination system employing β-recombinase acting on six recognition sequences (β-rec/six) in N. crassa, which allowed repetitive site-specific gene deletion and marker recycling. We report generating the mus-51 deletion strain using this system, recycling the marker cassette, and subsequently deleting the global transcriptional regulator gene cre-1.

journal_name

J Microbiol Methods

journal_title

Journal of microbiological methods

authors

Szewczyk E,Kasuga T,Fan Z

doi

10.1016/j.mimet.2012.12.004

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

236-43

issue

3

eissn

0167-7012

issn

1872-8359

pii

S0167-7012(12)00400-9

journal_volume

92

pub_type

杂志文章

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