Abstract：
:Due to technical problems, biofilm biomasses are difficult to be precisely determined. One reliable strategy is based on the colorimetry of formazan compounds derived from tetrazolium salt reduction. XTT presents some desirable properties that make the biofilm measurements easier. However, cells entrapped within the extracellular matrixes normally do not metabolize the tetrazolium equally, leading to underestimation of cell contents. This study evaluated the effectiveness of D-glutamine, a plerotic substrate of tricarboxilic acid cycle (TAC), as inducer of XTT reduction. The metabolic activities of aerobic and anaerobic 48 h-old monospecific biofilms of Pseudomonas aeruginosa ATCC®27853™, Klebsiella pneumoniae ATCC®13883™, Staphylococcus epidermidis ATCC®12228™, Streptococcus mutans ATCC®25175™, and Candida albicans SC5314 were evaluated. Results showed that D-glutamine 50 mM (for P. aeruginosa, K. pneumoniae, and S. epidermidis) and 25 mM (for S. mutans and C. albicans) may enhance the detection of soluble formazan in a significant manner, what becomes the XTT reduction assay more robust.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Gobor T,Corol G,Ferreira LE,Rymovicz AU,Rosa RT,Campelo PM,Rosa EAdoi
10.1016/j.mimet.2010.12.018subject
Has Abstractpub_date
2011-02-01 00:00:00pages
299-306issue
2eissn
0167-7012issn
1872-8359pii
S0167-7012(10)00449-5journal_volume
84pub_type
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