Pentylenetetrazole-induced bursting activity and cellular protein phosphorylation in snail neurons.

Abstract:

:To elucidate the intracellular mechanism of seizure discharge, phosphorylation of cellular protein during pentylenetetrazole (PTZ)-induced bursting activity in snail neurons was investigated. PTZ markedly enhanced the phosphorylation of proteins of 34,000 and 50,000 molecular weight. Similar effects were observed by application of a calcium ionophore, A23187. Calmodulin antagonist, N-(6-aminohexyl)-5-chloronaphthalenesulfonamide hydrochloride (W-7), inhibited the PTZ-induced increased phosphorylation of these two proteins. Dibutyryl cyclic AMP and iosobutylmethylxanthine (IBMX) showed no significant effect on the phosphorylation pattern. Bath application of the calcium ionophore produced bursting activity followed by long-lasting hyperpolarization. Bath application of W-7 completely inhibited the PTZ-induced bursting activity. These results suggest that the phosphorylation of proteins of 34,000 and 50,000 in molecular weight is related to the generation of bursting activity by PTZ.

journal_name

Brain Res

journal_title

Brain research

authors

Onozuka M,Imai S,Sugaya E

doi

10.1016/0006-8993(86)91395-8

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

33-9

issue

1

eissn

0006-8993

issn

1872-6240

pii

0006-8993(86)91395-8

journal_volume

362

pub_type

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