Abstract:
:Recent studies indicate that expansion of NKG2C-positive natural killer (NK) cells is associated with human cytomegalovirus (HCMV); however, their activity in response to HCMV-infected cells remains unclear. We show that NKG2C(hi) CD57(hi) NK cells gated on CD3(neg) CD56(dim) cells can be phenotypically identified as HCMV-induced NK cells that can be activated by HCMV-infected cells. Using HCMV-infected autologous macrophages as targets, we were able to show that these NKG2C(hi) CD57(hi) NK cells are highly responsive to HCMV-infected macrophages only in the presence of HCMV-specific antibodies, whereas they are functionally poor effectors of natural cytotoxicity. We further demonstrate that NKG2C(hi) CD57(hi) NK cells are intrinsically responsive to signaling through CD16 cross-linking. Our findings show that the activity of pathogen-induced innate immune cells can be enhanced by adaptive humoral immunity. Understanding the activity of NKG2C(hi) CD57(hi) NK cells against HCMV-infected cells will be of relevance for the further development of adoptive immunotherapy.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wu Z,Sinzger C,Frascaroli G,Reichel J,Bayer C,Wang L,Schirmbeck R,Mertens Tdoi
10.1128/JVI.01096-13subject
Has Abstractpub_date
2013-07-01 00:00:00pages
7717-25issue
13eissn
0022-538Xissn
1098-5514pii
JVI.01096-13journal_volume
87pub_type
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