Abstract:
:Genetic variation of the more complex retroviruses in the human T-cell leukemia virus/bovine leukemia virus (HTLV/BLV) group is less than in some other retroviral genera. To test whether reverse transcription of HTLV/BLV group members is less error prone than that of members of other groups, we developed an assay for detecting forward mutations in BLV, similar to that developed for the simpler spleen necrosis virus (SNV). We used this system to study the rates and types of mutations that occur during a single replication cycle. We found that BLV reverse transcription is approximately two and one-half times less error prone than SNV reverse transcription (4.8 x 10(-6) versus 1.2 x 10(-5) mutation per bp per cycle, respectively). The relative numbers of all types of observed mutations (that is, base pair substitutions, frameshifts, deletions, and deletions with insertions) were similar for BLV and SNV.
journal_name
J Viroljournal_title
Journal of virologyauthors
Mansky LM,Temin HMdoi
10.1128/JVI.68.1.494-499.1994subject
Has Abstractpub_date
1994-01-01 00:00:00pages
494-9issue
1eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::Regulatory T (T(R)) cells maintain tolerance to self-antigens and control immune responses to alloantigens after organ transplantation. Here, we show that CD4(+) CD25(+) human T(R) cells suppress virus-specific T-cell responses. Depletion of T(R) cells from peripheral blood mononuclear cells enhances T-cell responses ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2454-2459.2004
更新日期:2004-03-01 00:00:00
abstract::The vif gene of human immunodeficiency virus type 1 (HIV-1) encodes a basic Mr 23,000 protein that is necessary for production of infectious virions by nonpermissive cells (human lymphocytes and macrophages) but not by permissive cells such as HeLa-CD4. It had been proposed that permissive cells may contain an unident...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10251-10255.1998
更新日期:1998-12-01 00:00:00
abstract::After isolation, the DNA of simian virus 40 appeared as a negative supertwist (form I) or as an open circle with at least one single-strand scission (form II). Under the denaturation conditions usually applied, such as heating in the presence of formaldehyde or application of alkali, form I molecules could appear as "...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.585-598.1975
更新日期:1975-03-01 00:00:00
abstract::Experiments with St. Louis encephalitis (SLE) virus have shown that neutral red enhances the plaque size in duck embryo cell cultures. This may represent a new method for genetic studies of SLE virus population. In a mosquito pool specimen NR(+) and NR(-) particles were demonstrated. By intracerebral passage in mice, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.3.178-181.1968
更新日期:1968-03-01 00:00:00
abstract::Some retroviruses with an extended repeat (R) region encode the polyadenylation signal within the R region such that this signal is present at both the 5' and 3' ends of the viral transcript. This necessitates differential regulation to either repress recognition of the 5' polyadenylation signal or enhance usage of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.81-91.1999
更新日期:1999-01-01 00:00:00
abstract::10A1 murine leukemia virus can enter cells by using either of two different cell surface phosphate transport proteins, the gibbon ape leukemia virus receptor Glvr-1 (Pit-1) or the amphotropic retrovirus receptor Ram-1 (Pit-2). Glvr-1 and Ram-1 are widely expressed in different tissues, but the relative amounts of each...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5564-5571.1996
更新日期:1996-08-01 00:00:00
abstract:UNLABELLED:The disease mechanisms associated with onset and secondary effects of rotavirus (RV) diarrhea remain to be determined and may not be identical. In this study, we investigated whether onset of RV diarrhea is associated with increased intestinal permeability and/or motility. To study the transit time, fluoresc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02927-13
更新日期:2014-03-01 00:00:00
abstract::Beyond their role in cellular RNA metabolism, DExD/H-box RNA helicases are hijacked by various RNA viruses in order to assist replication of the viral genome. Here, we identify the DExH-box RNA helicase 9 (DHX9) as a binding partner of chikungunya virus (CHIKV) nsP3 mainly interacting with the C-terminal hypervariable...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01764-18
更新日期:2019-02-05 00:00:00
abstract::The purpose of this study was to define the relationship between herpes simplex virus (HSV) latency and in vivo ganglionic reactivation. Groups of mice with numbers of latently infected neurons ranging from 1.9 to 24% were generated by varying the input titer of wild-type HSV type 1 strain 17syn+. Reactivation of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6888-6892.1998
更新日期:1998-08-01 00:00:00
abstract::The biological and genetic characteristics of murine leukemia viruses (MuLV) derived from leukemic and normal HRS/J mice were studied. T1-oligonucleotide fingerprinting and mapping of viral RNAs from unpassaged isolates revealed the presence of complex mixtures of viral genomes. MuLV that were purified by endpoint dil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.2.397-407.1984
更新日期:1984-05-01 00:00:00
abstract::We previously demonstrated that polyhedron formation (PF) mutants arise at a high frequency during serial passage of the Lymantria dispar nucleopolyhedrovirus (LdMNPV) in the L. dispar 652Y cell line (J. M. Slavicek, N. Hayes-Plazolles, and M. E. Kelly, Biol. Control 5:251-261, 1995). Most of these PF mutants exhibite...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1097-1106.1997
更新日期:1997-02-01 00:00:00
abstract::Hepatitis B virus (HBV) and woolly monkey hepatitis B virus (WMHBV) are primate hepadnaviruses that display restricted tissue and host tropisms. Hepatitis D virus (HDV) particles pseudotyped with HBV and WMHBV envelopes (HBV-HDV and WM-HDV) preferentially infect human and spider monkey hepatocytes, respectively, there...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.9786-9798.2005
更新日期:2005-08-01 00:00:00
abstract::Gene expression of human immunodeficiency virus (HIV) is modulated by both cellular transcription factors, which bind to cis-acting regulatory elements in the HIV-1 long terminal repeat (LTR) and the viral transactivator, tat. The enhancer element in the HIV-1 LTR which extends from -103 to -82 is critical for gene ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1002-1009.1994
更新日期:1994-02-01 00:00:00
abstract::Understanding the mechanism(s) of action of the hepatitis B virus (HBV)-encoded protein HBx is fundamental to elucidating the underlying mechanisms of chronic liver disease and hepatocellular carcinoma caused by HBV infection. In our continued attempts to identify cellular targets of HBx, we have previously reported t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2840-2846.2000
更新日期:2000-03-01 00:00:00
abstract::The entry of enveloped viruses into cells is normally mediated by fusion between viral and cellular membranes, in which the fusion peptide plays a crucial role. The fusion peptides of group II nucleopolyhedrovirus (NPV) F proteins are quite conserved, with a hydrophobic region located at the N terminal of the F(1) fra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00368-08
更新日期:2008-08-01 00:00:00
abstract::Packaging of type C retrovirus genomic RNAs into budding virions requires a highly specific interaction between the viral Gag precursor and unique cis-acting packaging signals on the full-length RNA genome, allowing the selection of this RNA species from among a pool of spliced viral RNAs and similar cellular RNAs. Th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5886-5896.1998
更新日期:1998-07-01 00:00:00
abstract::One characteristic of all positive-strand RNA viruses is the necessity to assemble viral RNA replication complexes on host intracellular membranes, a process whose molecular details are poorly understood. To study viral replication complex assembly we use the established model system of Flock House virus (FHV), which ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00040-09
更新日期:2009-05-01 00:00:00
abstract::We have examined the pathway of rhinovirus interaction with soluble intercellular adhesion molecule 1 (sICAM-1). Binding of sICAM-1 to rhinovirus serotypes 3 and 14 gives particles with sedimentation coefficients from 145 to 120S, depending on the amount of sICAM-1 bound. The formation of 120S particles is faster and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5882-5889.1994
更新日期:1994-09-01 00:00:00
abstract::Several viruses, including members of the gammaherpesvirus family, encode proteins that are secreted into the extracellular environment. We have identified an abundant 44-kDa secreted protein that is present in the supernatant of fibroblasts infected with murine gammaherpesvirus 68 (gammaHV68; also referred to as MHV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4524-4529.1999
更新日期:1999-05-01 00:00:00
abstract::Recently it has been shown that kaposica, an immune evasion protein of Kaposi's sarcoma-associated herpesvirus, inactivates complement by acting on C3-convertases by accelerating their decay as well as by acting as a cofactor in factor I-mediated inactivation of their subunits C3b and C4b. Here, we have mapped the fun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5850-5856.2005
更新日期:2005-05-01 00:00:00
abstract::Genetic bottlenecks are important events in the genetic diversification of organisms and colonization of new ecological niches. Repeated bottlenecking of RNA viruses often leads to fitness losses due to the operation of Muller's ratchet. Herein we use vesicular stomatitis virus to determine the transmission population...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.2869-2872.1995
更新日期:1995-05-01 00:00:00
abstract::We studied the effect of booster injections and the long-term immune response after injections of an anti-human immunodeficiency virus type 1 (HIV-1) lipopeptide vaccine. This vaccine was injected alone or with QS21 adjuvant to 28 HIV-uninfected volunteers. One month later, after a fourth injection of the vaccine, B- ...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/jvi.77.20.11220-11231.2003
更新日期:2003-10-01 00:00:00
abstract::Type 3 reovirus inhibits L cell DNA synthesis, whereas type 1 reovirus exerts little or no effect on L cell DNA synthesis. By using recombinant viruses containing both type 1 and type 3 double-standard RNA segments, we determined that one double-stranded RNA segment, the reovirus type 3 S1 double-stranded RNA segment ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.389-392.1981
更新日期:1981-04-01 00:00:00
abstract::Autographa californica nuclear polyhedrosis virus late and very late mRNAs are transcribed by an RNA polymerase consisting of four virus-encoded polypeptides: LEF-8, LEF-9, LEF-4, and p47. The 464-amino-acid LEF-4 subunit contains the signature motifs of GTP:RNA guanylyltransferases (capping enzymes). Here, we show th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10020-10028.1998
更新日期:1998-12-01 00:00:00
abstract::Nonsegmented negative-sense RNA viruses (mononegaviruses) control viral gene expression largely through a transcription gradient such that promoter-proximal genes are transcribed more abundantly than downstream genes. For some paramyxoviruses, naturally occurring differences in the levels of efficiency of transcriptio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.692-699.2004
更新日期:2004-01-01 00:00:00
abstract::A 4,500-bp BamHI fragment, located within the HindIII A segment of the vaccinia virus genome, was found to contain eight potential coding regions for polypeptides of 78 to 346 amino acids. The open reading frames with 133, 346, and 125 codons were homologous to profilin (an actin-binding protein), 3-beta-hydroxysteroi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4598-4608.1991
更新日期:1991-09-01 00:00:00
abstract::Semlike forest virus capsid protein cosedimented with the large ribosomal subunit at 60S in sucrose gradients after treatment of cytoplasm from infected cells with Triton X-100 and EDTA. In CsCl gradients the capsid protein banded with the subunit at a density of 1.56 to 1.57 g/cm3. Most of the capsid protein could be...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.203-210.1976
更新日期:1976-10-01 00:00:00
abstract::The 104-S monoclonal antibody immunoprecipitated from herpes simplex virus type 2 (HSV-2)-infected cell extracts the 75,000-molecular-weight glycoprotein gF and its 65,000-molecular-weight precursor (pgF). The precursor pgF was sensitive to endoglycosidase H digestion, indicating the presence of high mannose-type olig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.1.185-192.1983
更新日期:1983-07-01 00:00:00
abstract::The cellular transcription factors Sp1 and NF-kappaB were upregulated shortly after the binding of purified live or UV-inactivated human cytomegalovirus (HCMV) to the cell surface. The rapid time frame of transcription factor induction is similar to that seen in other systems in which cellular factors are induced foll...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5051-5059.1997
更新日期:1997-07-01 00:00:00
abstract::The mRNAs of Bunyamwera virus (BUNV), the prototype of the Bunyaviridae family, possess a 5' cap structure but lack a 3' poly(A) tail, a common feature of eukaryotic mRNAs that greatly enhances translation efficiency. Viral mRNAs also contain untranslated regions (UTRs) that flank the coding sequence. Using model viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02201-08
更新日期:2009-04-01 00:00:00