Abstract:
:Determinants of glycosylation site usage were explored by using the hemagglutinin-neuraminidase (HN) glycoprotein of the paramyxovirus Newcastle disease virus. The amino acid sequence of the HN protein, a type II glycoprotein, has six N-linked glycosylation addition sites, G1 to G6, two of which, G5 and G6, are not used for the addition of carbohydrate (L. McGinnes and T. Morrison, Virology 212:398-410, 1995). The sequence of this protein also has 13 cysteine residues in the ectodomain (C2 to C14). Mutation of either cysteine 13 or cysteine 14 resulted in the addition of another oligosaccharide chain to the protein. These cysteine residues flank the normally unused G6 glycosylation addition site, and mutation of the G6 site eliminated the extra glycosylation found in the cysteine mutants. These results suggested that failure to form an intramolecular disulfide bond resulted in the usage of a normally unused glycosylation site. This conclusion was confirmed by preventing cotranslational disulfide bond formation in cells by using dithiothreitol. Under these conditions, the wild-type protein acquired extra glycosylation, which was eliminated by mutation of the G6 site. These results suggest that localized folding events on the nascent chain, such as disulfide bond formation, which block access to the oligosaccharyl transferase are a determinant of glycosylation site usage.
journal_name
J Viroljournal_title
Journal of virologyauthors
McGinnes LW,Morrison TGdoi
10.1128/JVI.71.4.3083-3089.1997subject
Has Abstractpub_date
1997-04-01 00:00:00pages
3083-9issue
4eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::Herpesvirus nucleocapsids traverse the nuclear envelope into the cytoplasm in a process called nuclear egress that includes disruption of the nuclear lamina. In several herpesviruses, a key player in nuclear egress is a complex of two proteins, whose homologs in human cytomegalovirus (HCMV) are UL50 and UL53. However,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02358-13
更新日期:2014-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.12300-12311.2002
更新日期:2002-12-01 00:00:00
abstract:UNLABELLED:Little is known about the antiviral response in mollusks. As in other invertebrates, the interferon signaling pathways have not been identified, and in fact, there is a debate about whether invertebrates possess antiviral immunity similar to that of vertebrates. In marine bivalves, due to their filtering act...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00591-16
更新日期:2016-08-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6559-6564.1998
更新日期:1998-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01226-08
更新日期:2008-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.10074-10085.2004
更新日期:2004-09-01 00:00:00
abstract::The simian picornaviruses were isolated from various primate tissues during the development of general tissue culture methods in the 1950s to 1970s or from specimens derived from primates used in biomedical research. Twenty simian picornavirus serotypes are recognized, and all are presently classified within the Enter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.1244-1251.2002
更新日期:2002-02-01 00:00:00
abstract::A prerequisite for understanding the molecular function of the human cytomegalovirus (HCMV) gH (UL75)-gL (UL115) complex is a detailed knowledge of the structure of this complex in its functional form, as it is present in mature virions. The gH protein is known to be a component of a 240-kDa envelope complex designate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5391-5398.1997
更新日期:1997-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.6.946-955.1972
更新日期:1972-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.47-56.2005
更新日期:2005-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01859-20
更新日期:2020-12-22 00:00:00
abstract::Profound alterations in host cell nuclear architecture accompany the lytic phase of Epstein-Barr virus (EBV) infection. Viral replication compartments assemble, host chromatin marginalizes to the nuclear periphery, cytoplasmic poly(A)-binding protein translocates to the nucleus, and polyadenylated mRNAs are sequestere...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01254-18
更新日期:2018-09-26 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2483-2490.1998
更新日期:1998-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10421-10430.2001
更新日期:2001-11-01 00:00:00
abstract::Infectious hematopoietic necrosis virus (IHNV) is a rhabdovirus that produces an acute, lethal infection in rainbow trout (Oncorhynchus mykiss). Fish that survive infection cease to produce detectable infectious virus at approximately 46 days after infection, yet there is evidence that survivor fish continue to harbor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.843-849.1999
更新日期:1999-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.1.267-272.1989
更新日期:1989-01-01 00:00:00
abstract::Myeloblastosis-associated virus type 1 (MAV1) derived from a molecular clone of infectious proviral DNA (B. Perbal, J. S. Lipsick, J. Svoboda, R. F. Silva, and M. A. Baluda, J. Virol. 56:240-244, 1985) was shown to specifically induce nephroblastoma in chickens and therefore belongs to the MAV-N class. We show that ne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1803-1807.1989
更新日期:1989-04-01 00:00:00
abstract::The glycoproteins of several enveloped viruses, grown in a variety of cell types, are labeled with 35SO4(-2), whereas the nonglycosylated proteins are not. This was shown for the HN and F glycoproteins of SV5 and Sendai virus, the E1 and E2 glycoproteins of Sindbis virus, and for the major glycoprotein, gp69, as well ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.859-866.1975
更新日期:1975-10-01 00:00:00
abstract::Whether a genetic informational nucleic acid is required for the infectivity of transmissible spongiform encephalopathies is central to the debate about the infectious agent. Here we report that an infectious prion formed with bacterially expressed recombinant prion protein plus synthetic polyriboadenylic acid and syn...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06216-11
更新日期:2012-02-01 00:00:00
abstract::A series of plasmids containing hepatitis A virus (HAV) cDNA was constructed such that positive-strand HAV RNA could be transcribed with T7 RNA polymerase. The plasmids differed in the number of 5'-terminal nucleotides representing the junctions between vectors and HAV sequences that were present in the transcripts. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2757-2760.1991
更新日期:1991-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02205-13
更新日期:2014-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9515-9520.1999
更新日期:1999-11-01 00:00:00
abstract::Adenovirus (Ad) replicative complexes form at discrete sites on the nuclear matrix (NM) via an interaction mediated by the precursor of the terminal protein (pTP). The identities of cellular proteins involved in these complexes have remained obscure. We present evidence that pTP binds to a multifunctional pyrimidine b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2896-2904.1998
更新日期:1998-04-01 00:00:00
abstract::Certain murine leukemia viruses (MLVs) can induce progressive noninflammatory spongiform neurodegeneration similar to that caused by prions. The primary MLV determinants responsible have been mapped to within the env gene; however, it has remained unclear how env mediates disease, whether non-Env viral components are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02210-10
更新日期:2011-03-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) readily infects both humans and chimpanzees, but the pathologic outcomes of infection in these two species differ greatly. In attempts to identify virus-cell interactions that might account for this differential pathogenicity, chimpanzee peripheral blood lymphocytes and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3344-3348.1991
更新日期:1991-06-01 00:00:00
abstract::Recent studies have demonstrated that influenza A virus infection activates the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway by binding of influenza NS1 protein to the p85 regulatory subunit of PI3K. Our previous study proposed that two polyproline motifs in NS1 (amino acids 164 to 167 [PXXP], SH3 bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01427-07
更新日期:2007-12-01 00:00:00
abstract::When influenza virus monoclonal antibody-resistant (mar) mutants are selected by incubation in vitro with excess antibody, 90 to 99% of the mutants are not detectable. This observation may be explained by encapsidation of mar mutant RNAs within phenotypically wild-type envelopes. This phenotypic hiding can be revealed...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.4107-4109.1989
更新日期:1989-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01759-06
更新日期:2007-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1614-1622.2000
更新日期:2000-02-01 00:00:00
abstract::Capsid (core) assembly is essential for hepatitis B virus (HBV) replication. We hypothesize that assembly kinetics and stability are tuned for optimal viral replication, not maximal assembly. Assembly effectors (AEfs) are small molecules proposed to disrupt this balance by inappropriately enhancing core assembly. Guid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03014-12
更新日期:2013-03-01 00:00:00