Abstract：
:Recent advances in inductively coupled plasma mass spectrometry (ICP-MS) as applied in mass cytometry, enabled its broad applicability to life science research. Mass cytometry enables the high-dimensional characterization of cellular systems by simultaneously measuring dozens of metal isotope reporter labeled antibodies bound to cell components. With the ability to simultaneously interrogate an unprecedented number of molecular components on a per cell basis, it offers the possibility to gain better understanding of single cell biology in heterogeneous samples. To upscale this single cell information to screening approaches by mass cytometry, a cell-based multiplexing technique, called mass-tag cellular barcoding (MCB), was developed. MCB enables the simultaneous analysis of multiple cell samples by using n metal ion tags to multiplex up to 2 (n) samples. Different mass tag combinations are used to label individual cell samples with a unique mass barcode that allows multiple samples to be combined and immunostained together for a single analysis on the mass cytometer. Taken together, MCB enables increased sample throughput, reduces antibody consumption, and increases the overall data quality. In this chapter, we describe the MCB to array the samples in a 96-well format that allows for medium-scale profiling/screening experiments to be run on a standard mass cytometer.
journal_name
Curr Top Microbiol Immunoljournal_title
Current topics in microbiology and immunologyauthors
Zivanovic N,Jacobs A,Bodenmiller Bdoi
10.1007/82_2013_335subject
Has Abstractpub_date
2014-01-01 00:00:00pages
95-109eissn
0070-217Xissn
2196-9965journal_volume
377pub_type
杂志文章abstract：:Phosphoinositide 3-kinases (PI 3-kinases) are activated by growth factor and hormone receptors, and regulate cell growth, survival, motility, and responses to changes in nutritional conditions (Engelman et al. 2006). PI 3-kinases have been classified according to their subunit composition and their substrate specifici...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2010_52
更新日期：2010-01-01 00:00:00
abstract：:Inflammatory responses to microbial and non-microbial stimuli involve coordinate changes in the expression of hundreds of genes. The inflammatory gene expression programs display two important features: first, alternative stimuli affect both a large set of common genes and a variable number of stimulus-specific genes....
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2010_106
更新日期：2011-01-01 00:00:00
abstract：:Certain vertebrates are capable of regenerating parts of the eye, including the lens. Depending on the species, two principal forms of in vivo lens regeneration have been described wherein the new lens arises from either the pigmented epithelium of the dorsal iris or the cornea epithelium. These forms of lens regenera...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2012_289
更新日期：2013-01-01 00:00:00
abstract：:An evolution in modern malaria research occurred with the completion of the Plasmodium falciparum genome project and the onset and application of novel post-genomic technologies. Corresponding with these technological achievements are improvements in accessing and purifying parasite material from 'hard-to-reach' stage...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/3-540-29088-5_16
更新日期：2005-01-01 00:00:00
abstract：:T cell activation begins with the interaction between an antigen-specific T cell and an antigen-presenting cell (APC). This interaction results in the formation of the immunological synapse, which had been considered to be responsible for antigen recognition and T cell activation. Recent advances in imaging analysis h...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-03858-7_5
更新日期：2010-01-01 00:00:00
abstract：:Severe acute respiratory syndrome (SARS) was the first pandemic transmissible disease of previously unknown aetiology in the twenty-first century. Early epidemiologic investigations suggested an animal origin for SARS-CoV. Virological and serological studies indicated that masked palm civets ( Paguma larvata), togethe...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-540-70962-6_13
更新日期：2007-01-01 00:00:00
abstract：:Autophagy plays diverse roles in cellular adaptation to stress and promotes vital housekeeping functions by recycling unused or damaged organelles and proteins. As an innate immune defense pathway, autophagy also protects against infection with diverse pathogens, including viruses. Autophagy combats infections with bo...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-00302-8_13
更新日期：2009-01-01 00:00:00
abstract：:Promoting remyelination following injury to the central nervous system (CNS) promises to be an effective neuroprotective strategy to limit the loss of surviving axons and prevent disability. Studies confirm that multiple sclerosis (MS) and spinal cord injury lesions contain myelinating cells and their progenitors. Rec...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-540-73677-6_9
更新日期：2008-01-01 00:00:00
abstract：:Proteasomes are highly abundant cytosolic and nuclear protease complexes that degrade most intracellular proteins in higher eukaryotes and appear to play a major role in the cytosolic steps of MHC class I antigen processing. This review summarizes the knowledge of the role of proteasomes in antigen processing and the ...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-59414-4_5
更新日期：2002-01-01 00:00:00
abstract：:The surface membrane molecule CD5 is expressed on mature T cells and on the B-1a subpopulation of B cells. These CD5 positive B cells express an antibody repertoire with a relatively high frequency of self-reactivity. There is uncertainty about the origins of CD5 B cells and the reasons for this are reviewed. Recent r...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-79275-5_26
更新日期：1995-01-01 00:00:00
abstract：:Tuberculosis (TB) is caused by infection with Mycobacterium tuberculosis (Mtb) and represents one of the most relevant bacterial diseases worldwide. Recent advances have yielded new insights into the molecular basis of the immune response required for restriction of the pathogen and also highlighted determinants of im...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-41171-2_9
更新日期：2016-01-01 00:00:00
abstract：:The ability to regulate and even target mutagenesis is an extremely valuable cellular asset. Enzyme-catalyzed DNA cytosine deamination is a molecular strategy employed by vertebrates to promote antibody diversity and defend against foreign nucleic acids. Ten years ago, a family of cellular enzymes was first described ...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-37765-5_1
更新日期：2013-01-01 00:00:00
abstract：:The possibility that a new form of human prion disease, variant Creutzfeldt-Jakob disease (vCJD) had occurred in the UK was first raised by the identification of a small number of cases with unusual clinical characteristics. Atypical features included a young age at death, a predominantly psychiatric presentation, a r...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-662-08441-0_5
更新日期：2004-01-01 00:00:00
abstract：:Axonal transport ensures long-range delivery of essential components and signals between proximal and distal areas of the neuron, and it is crucial for neuronal homeostasis and survival. Several pathogens and virulence factors use this route to gain access to the central nervous system, exploiting the complex and stil...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-33570-9_5
更新日期：2013-01-01 00:00:00
abstract：:Primary varicella-zoster virus (VZV) infection (varicella) induces VZV-specific antibody and VZV-specific T cell-mediated immunity. T cell-mediated immunity, which is detected within 1-2 weeks after appearance of rash, and consists of both CD4 and CD8 effector and memory T cells, is essential for recovery from varicel...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2010_31
更新日期：2010-01-01 00:00:00
abstract：:The development of bacterial resistance against current antibiotic drugs necessitates a continuous renewal of the arsenal of efficacious drugs. This imperative has not been met by the output of antibiotic research and development of the past decades for various reasons, including the declining efforts of large pharma ...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2016_501
更新日期：2016-01-01 00:00:00
abstract：:As the largest RNA virus, coronavirus replication employs complex mechanisms and involves various viral and cellular proteins. The first open reading frame of the coronavirus genome encodes a large polyprotein, which is processed into a number of viral proteins required for viral replication directly or indirectly. Th...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/3-540-26765-4_4
更新日期：2005-01-01 00:00:00
abstract：:This review will focus on the principal clinical and pathophysiological features of the anaemia of falciparum malaria, including the problems of treating malarial anaemia, and also will suggest how recent advances in genomics may help our understanding of cellular and molecular mechanisms underlying this syndrome. ...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/3-540-29088-5_6
更新日期：2005-01-01 00:00:00
abstract：:Wild aquatic bird populations have long been considered the natural reservoir for influenza A viruses with virus transmission from these birds seeding other avian and mammalian hosts. While most evidence still supports this dogma, recent studies in bats have suggested other reservoir species may also exist. Extensive ...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2014_396
更新日期：2014-01-01 00:00:00
abstract：:Currently there is no commercial Group A Streptococcus (GAS; S. pyogenes) vaccine available. The development of safe GAS vaccines is challenging, researchers are confronted with obstacles such as the occurrence of many unique serotypes (there are greater than 150 M types), antigenic variation within the same serotype,...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2012_284
更新日期：2013-01-01 00:00:00
abstract：:In summary, the complement system has evolved an important function in regulation of humoral immunity to T-dependent antigens. Covalent attachment of activated C3 to antigen alters its fate by enhancing uptake on the surface of FDC via CD21/CD35; and by enhancing signal transduction via the B cell coreceptor CD21/CD19...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-60162-0_8
更新日期：1999-01-01 00:00:00
abstract：:A critical function of the thymus is to help enforce tolerance to self. The importance of central tolerance in preventing autoimmunity has been enlightened by a deeper understanding of the interactions of developing T cells with a diverse population of thymic antigen presenting cell populations. Furthermore, there has...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2013_321
更新日期：2014-01-01 00:00:00
abstract：:The field of autophagy research has advanced rapidly in recent years, with important discoveries made in relation to both molecular mechanisms and physiological functions. Initially, autophagy was thought to be primarily a response to starvation. Although this might be true in lower eukaryotes, this catabolic process ...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-00302-8_3
更新日期：2009-01-01 00:00:00
abstract：:IgG4-related disease (IgG4-RD) is a systemic disease characterized by elevated serum IgG4 levels and a strong infiltration of IgG4-positive plasma cells in various organs. IgG4-RD patients also frequently suffer from allergic diseases, including asthma and atopic dermatitis. It is well known that T helper type 2 (Th2)...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2016_40
更新日期：2017-01-01 00:00:00
abstract：:Measles virus (MV) has two envelope glycoproteins, the hemagglutinin (H) and fusion protein, which are responsible for attachment and membrane fusion, respectively. Signaling lymphocyte activation molecule (SLAM, also called CD150), a membrane glycoprotein expressed on immune cells, acts as the principal cellular rece...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-540-70523-9_2
更新日期：2009-01-01 00:00:00
abstract：:The incidence of Kaposi sarcoma (KS) related to Kaposi sarcoma-associated herpesvirus (KSHV/HHV-8) after organ transplantation is 500-1000 times greater than in the general population, and its occurrence is associated with immunosuppressive therapy. The reported incidence of posttransplant KS ranges from 0.5% to 5%, d...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/978-3-540-34344-8_9
更新日期：2007-01-01 00:00:00
abstract：:Collective knowledge regarding the occurrence of influenza among swine is incomplete due to inconsistent surveillance of swine populations. In this chapter, we review what surveillance activities exist and some of the practical challenges encountered. Furthermore, to support robust surveillance activities, accurate la...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2012_220
更新日期：2013-01-01 00:00:00
abstract：:Theoretical modeling of quasispecies has progressed in several directions. In this chapter, we review the works of Emmanuel Tannenbaum, who, together with Eugene Shakhnovich at Harvard University and later with colleagues and students at Ben-Gurion University in Beersheva, implemented one of the more useful approaches...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2015_456
更新日期：2016-01-01 00:00:00
abstract：:Group A streptococcal (Streptococcus pyogenes) infections remain important causes of medical and public health morbidity and mortality even during the early twenty-first century. Although most often concentrated in socially/economically disadvantaged populations, the problems remain significant in both industrializing...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2012_306
更新日期：2013-01-01 00:00:00
abstract：:During the last two decades, researchers have developed robust systems for recombinant subunit vaccine production in plants. Stably and transiently transformed plants have particular advantages that enable immunization of humans and animals via mucosal delivery. The initial goal to immunize orally by ingestion of plan...
journal_title：Current topics in microbiology and immunology
pub_type： 杂志文章,评审
doi：10.1007/82_2011_158
更新日期：2012-01-01 00:00:00
