Abstract:
:RNA interference is an effective and naturally occurring post-transcriptional gene regulatory mechanism. This mechanism involves the degradation of a target messenger RNA (mRNA) through the introduction of short interfering RNA (siRNA) that is complementary to the target mRNA. The application of siRNA-based therapeutics is limited by the development of an effective delivery system, as naked siRNA is unstable and cannot penetrate the cell membrane. In this study, we investigated the use of cationic niosomes (CN) prepared by microfluidic mixing for siRNA delivery. In an in vitro model, these vesicles were able to deliver anti-luciferase siRNA and effectively suppress luciferase expression in B16-F10 mouse melanoma cells. More importantly, in an in vivo mouse model, intratumoral administration of CN-carrying anti-luciferase siRNA led to significant suppression of luciferase expression compared with naked siRNA. Thus, we have established a novel and effective system for the delivery of siRNA both in vitro and in vivo, which shows high potential for future application of gene therapeutics.
journal_name
J Liposome Resjournal_title
Journal of liposome researchauthors
Obeid MA,Dufès C,Somani S,Mullen AB,Tate RJ,Ferro VAdoi
10.1080/08982104.2018.1531424subject
Has Abstractpub_date
2019-09-01 00:00:00pages
229-238issue
3eissn
0898-2104issn
1532-2394journal_volume
29pub_type
杂志文章abstract::Pulmonary lung targeting finds applications in drug delivery to the lung itself and to other body organs, via blood circulation following transfer across alveolar membranes. Understanding pulmonary drug delivery systems towards improving their efficacy needs identification of particle sizes of relevance and elucidatio...
journal_title:Journal of liposome research
pub_type: 杂志文章,评审
doi:10.3109/08982104.2013.802332
更新日期:2013-12-01 00:00:00
abstract::The current investigation aims to develop and evaluate novel ocular proniosomal gels of lomefloxacin HCl (LXN); in order to improve its ocular bioavailability for the management of bacterial conjunctivitis. Proniosomes were prepared using different types of nonionic surfactants solely and as mixtures with Span 60. The...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2016.1167737
更新日期:2017-06-01 00:00:00
abstract::Sonication is a simple method for reducing the size of liposomes. We report the size distributions of liposomes as a function of sonication time using three different techniques. Liposomes, mildly sonicated for just 30 sec, had bimodal distributions when surface-weighted with modes at about 140 and 750 nm. With extend...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982100500528842
更新日期:2006-01-01 00:00:00
abstract::Adenosine triphosphate (ATP)-binding cassette (ABC) transporters play a key role in the development of multidrug resistance (MDR) in cancer cells. P-glycoprotein (P-gp) and multidrug resistance-associated protein 1 (MRP1) are important proteins in this superfamily which are widely expressed on the membranes of multidr...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982104.2016.1185731
更新日期:2017-06-01 00:00:00
abstract::Liposomal vinorelbine formulation is desirable, as it might improve the therapeutic activity of vinorelbine. However, because of its lipophilic and membrane-permeable properties, vinorelbine is hard to be formulated into liposomes using conventional drug-loading technologies. To improve vinorelbine retention, ammonium...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2011.584880
更新日期:2012-03-01 00:00:00
abstract::The use of arsenic-containing compounds in cancer therapy is currently being re-considered, after the recent approval of arsenic trioxide (Trisenox) for the treatment of relapsed promyelocytic leukemia (PML). In an attempt to prepare a carrier system to minimize the toxicity of this drug, the aim of this study is to p...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1081/lpr-120039661
更新日期:2004-01-01 00:00:00
abstract::The luciferase gene expression of lipoplexes, a liposome containing luciferase plasmid (pCMVLuc), in HeLa cell lines, was investigated. Cationic liposomes were prepared by the chloroform film method with sonication. The lipoplex was formed by loading the liposome with pCMVLuc. The lipoplex with an optimal weight ratio...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982100802642457
更新日期:2009-01-01 00:00:00
abstract::The influence of esters based on gamma-aminobutyric acid (GABA) and mono-/bicyclic terpenoids on membrane structure was investigated. The mechanism of action for terpenoid esters on phospholipids of artificial membranes and lipids isolated from the rat stratum corneum was studied by fluorescence and FT-IR spectroscopy...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982104.2018.1538238
更新日期:2019-09-01 00:00:00
abstract::The aim of present study was to develop conventional and PEGylated (long circulating), liposomes containing anastrozole (ANS) for effective treatment of breast cancer. ANS is a third-generation non-steroidal aromatase inhibitor of the triazole class used for the treatment of advanced and late-stage breast cancer in po...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2015.1029493
更新日期:2016-01-01 00:00:00
abstract::Doxorubicin, as a widely used chemotherapeutic, always causes multidrug resistance in human cancer cells. To circumvent drug resistance, we developed a novel formulation where doxorubicin hydrochloride (DOX) and chloroquine phosphate (CQ) were simultaneously loaded into liposomes by a pH-gradient method where CQ playe...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2012.684150
更新日期:2012-09-01 00:00:00
abstract::The aim of this study was to establish the mechanisms of action of a novel liposomal nitric oxide (NO) carrier on large-conductance Ca2+-activated channels (BKCa or Maxi-K) expressed in vascular smooth muscle cells (VSMCs) isolated from the rat main pulmonary artery (MPA). Experimental design comprised of both whole-c...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982104.2020.1863424
更新日期:2021-01-08 00:00:00
abstract::Propylthiouracil, a lyophobic drug with an antiproliferative activity, was formulated into niosomes using various classes of nonionic surfactants. Feasibility of vesicle formation by the sonication method was evaluated. Size and size distribution was measured by laser diffraction. Entrapment and drug release over 24 h...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982100600992542
更新日期:2006-01-01 00:00:00
abstract::Skin is considered the most accessible organ of the body because of its underlying capillary network. However, stratum corneum (SC), the upper most layer of skin, represents major diffusional barrier for most drugs. Hence, the use of edge activators (EAs) in designing novel elastic vesicles is hypothesized to impart t...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982104.2018.1430831
更新日期:2019-03-01 00:00:00
abstract::Andrographis paniculata is a medicinal herb used extensively for various ailments and contains therapeutically active phytoconstituent, andrographolide (AN). Although hepatoprotective activity of AN is established, but their bioavailability is restricted due to its rapid clearance. The aim of this study, therefore, wa...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2012.753456
更新日期:2013-06-01 00:00:00
abstract::In this study we successfully entrapped 5-aminolevulinic acid (ALA) in liposome, although it exists as a zwitter ion. A molar ratio of 2:1:2.5 phosphatidyle-thanolamine (PE)/cholesterol/sodium stearate represented the best condition to achieve high entrapment efficiency (29.37 +/- 1.21%), and the average vehicle size ...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982100801893952
更新日期:2008-01-01 00:00:00
abstract:CONTEXT:The stability of liposomes in the form of dispersion is a major concern due to drug leakage and fusion or aggregation. The stability can be improved by lyophilization, but the stress induced by the lyophilization process can affect the integrity of liposomes. OBJECTIVE:The objective of this study was to evalua...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2014.992023
更新日期:2015-01-01 00:00:00
abstract::The aim of the present study was to characterize a liposome-based benzocaine (BZC) formulation designed for topical use on the oral mucosa and to evaluate its in vitro retention and permeation using the Franz-type diffusion cells through pig esophagus mucosa. To predict the effectiveness of new designed formulations d...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2012.742536
更新日期:2013-03-01 00:00:00
abstract::Naturally occurring antibodies to phospholipids and cholesterol are widespread; they occur commonly during the course of acute infections; they are not causally related to the anti-phospholipid syndrome; they have been associated with other clinical entities only as an epiphenomenon; and they have not been implicated ...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982100600848553
更新日期:2006-01-01 00:00:00
abstract::This work describes the preparation of a PEGylated niosomes-mediated drug delivery systems for Paeonol, thereby improving the bioavailability and chemical stability of Paeonol, prolonging its cellular uptake and enhancing its synergistic anti-cancer effects with 5-Fu. PEGylated niosomes, which are prepared from biocom...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982104.2016.1191021
更新日期:2017-06-01 00:00:00
abstract::The present study is envisaged to develop nanoethosomal formulation for enhanced topical delivery of amphotericin B (AmB) for the treatment of cutaneous fungal infections. AmB encapsulated nanoethosomes were prepared using mechanical dispersion method in a strength of 0.1% w/w similar to the strength of marketed topic...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2014.995670
更新日期:2015-01-01 00:00:00
abstract::A novel scalable liposome preparation technique for pharmaceutical application is presented. Previous experiments have shown that the concept of continuous crossflow injection is a promising approach. For the characterization of the process, we focus on the influencing parameters like the lipid concentration, the inje...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1081/lpr-120014761
更新日期:2002-01-01 00:00:00
abstract::Background and objective: The combination of two or more different mechanisms of drugs in the treatment of cancer has become one of the effective methods. The purpose of this study was to successfully prepare a non-viral delivery system that could efficiently co-delivery siRNA and gambogenic acid (GNA) to improve the ...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982104.2018.1473423
更新日期:2019-12-01 00:00:00
abstract::The aim of this study was to investigate the application of ultrasonic gas-filled liposomes in enhancing transfer for breast cancer-related antisense oligonucleotides in vitro. An antisense oligodeoxynucleotide (AS-ODN) sequence, HA2741, modified with luciferase reporter plasmid, was used in evaluating the enhancing e...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/03639040802509868
更新日期:2008-01-01 00:00:00
abstract::Sustained release depot systems have been widely investigated for their potential to improve the efficacy of subunit vaccines and reduce the requirement for boosting. The present study aimed to further enhance the immunogenicity of a sustained release vaccine by combining a depot formulation with a particulate antigen...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2011.637502
更新日期:2012-09-01 00:00:00
abstract::Valsartan (VLT) is a highly selective and orally active antihypertensive drug. However, its oral administration is associated with drawbacks like low bioavailability. The objective of this study was to design and develop a transdermal delivery system for VLT using ethosomal carriers to investigate their enhanced trans...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2012.753457
更新日期:2013-06-01 00:00:00
abstract::Methotrexate (MTX) is indicated in the symptomatic control of severe, recalcitrant, and disabling psoriasis. The oral or parenteral route of administration causes systemic toxicity. The topical route of delivery, though, reduces systemic toxicity and has limited applicability due to restricted permeability. Liposomal ...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982104.2010.492476
更新日期:2011-06-01 00:00:00
abstract::In the present work, we show that we obtained nanometric structures made of water, 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), cholesterol (Chol), and a mixture of ethoxylated and non-ethoxylated sorbitan fatty acid esters (Tween 20, Span 20, Tween 80, and Span 80) by mixing all of them near the cloud poi...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982104.2016.1174944
更新日期:2017-06-01 00:00:00
abstract::Intravenous injection of some liposomal drugs, diagnostic agents, micelles and other lipid-based nanoparticles can cause acute hypersensitivity reactions (HSRs) in a high percentage (up to 45%) of patients, with hemodynamic, respiratory and cutaneous manifestations. The phenomenon can be explained with activation of t...
journal_title:Journal of liposome research
pub_type: 杂志文章,评审
doi:10.1080/08982100701375118
更新日期:2007-01-01 00:00:00
abstract::The current study aimed to deliver minoxidil (2,4-diamino-6-piperidinopyrimidine 3-oxide; MXD), a potent hypertrichotic agent, into the pilosebaceous units, exploring the potential of the liposomal system. MXD-loaded liposomes of different compositions were prepared by a thin-film hydration technique and subsequently ...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1080/08982100903161449
更新日期:2010-06-01 00:00:00
abstract::The evidence in this communication indicate that, unlike resident Kupffer cells, newly recruited liver macrophages (following monocyte migration from the blood to the liver) use complement receptors to recognize and internalize stearylamine-incorporated liposomes. Within two weeks of hepatic residency complement recep...
journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.1081/lpr-120004778
更新日期:2002-02-01 00:00:00