Application of ultrasonic gas-filled liposomes in enhancing transfer for breast cancer-related antisense oligonucleotides: an experimental study.

Abstract:

:The aim of this study was to investigate the application of ultrasonic gas-filled liposomes in enhancing transfer for breast cancer-related antisense oligonucleotides in vitro. An antisense oligodeoxynucleotide (AS-ODN) sequence, HA2741, modified with luciferase reporter plasmid, was used in evaluating the enhancing effect of gas-filled liposomes for gene transfer in breast cancer cells. Some important factors on HA 2741 transfection efficiency, such as wave intensity, ultrasound duration, gas-filled liposome concentration, and HA2741 concentration, were tested, respectively. Transfection efficiency was detected by fluorescence microscopy. Cell viability was verified by propidium iodide assay. Reverse-transcriptase polymerase chain reaction and immunocytochemistry were used to detect the inhibitory effect of HA2741 on HER-2 expression. All the four factors (wave intensity, ultrasound duration, gas-filled liposome concentration, and HA2741 concentration) showed a positive effect on AS-ODN transfection efficiency. However, these factors had a negative effect on cell viability. Considering all the factors investigated, the maximum transfection efficiency with minimum cell viability achieved under 2% gas-filled liposome mixed with 80 nmol/L HA2741 for 30-second ultrasound exposure at -3.0 dB wave intensity, which gave an overall transfection efficiency exceeding 90% and a cell viability near 90%. Under controlled conditions, ultrasound-mediated AS-ODN transfer, enhanced by gas-filled liposomes, may represent an effective, safe avenue for cancer-related gene delivery.

journal_name

J Liposome Res

authors

Luo YK,Zhao YZ,Lu CT,Tang J,Li XK

doi

10.1080/03639040802509868

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

341-51

issue

4

eissn

0898-2104

issn

1532-2394

pii

905063038

journal_volume

18

pub_type

杂志文章
  • Nanoethosomal formulation for skin targeting of amphotericin B: an in vitro and in vivo assessment.

    abstract::The present study is envisaged to develop nanoethosomal formulation for enhanced topical delivery of amphotericin B (AmB) for the treatment of cutaneous fungal infections. AmB encapsulated nanoethosomes were prepared using mechanical dispersion method in a strength of 0.1% w/w similar to the strength of marketed topic...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2014.995670

    authors: Kaur L,Jain SK,Manhas RK,Sharma D

    更新日期:2015-01-01 00:00:00

  • Arsenic trioxide liposomes: encapsulation efficiency and in vitro stability.

    abstract::The use of arsenic-containing compounds in cancer therapy is currently being re-considered, after the recent approval of arsenic trioxide (Trisenox) for the treatment of relapsed promyelocytic leukemia (PML). In an attempt to prepare a carrier system to minimize the toxicity of this drug, the aim of this study is to p...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1081/lpr-120039661

    authors: Kallinteri P,Fatouros D,Klepetsanis P,Antimisiaris SG

    更新日期:2004-01-01 00:00:00

  • Sulfosalicylate mediates improved vinorelbine loading into LUVs and antineoplastic effects.

    abstract::Liposomal vinorelbine formulation is desirable, as it might improve the therapeutic activity of vinorelbine. However, because of its lipophilic and membrane-permeable properties, vinorelbine is hard to be formulated into liposomes using conventional drug-loading technologies. To improve vinorelbine retention, ammonium...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2011.584880

    authors: Li C,Cui J,Wang C,Cao J,Zhang L,Li Y,Liang M,Xiu X,Li Y,Wei N,Deng C

    更新日期:2012-03-01 00:00:00

  • Expression of luciferase plasmid (pCMVLuc) entrapped in DPPC/cholesterol/DDAB liposomes in HeLa cell lines.

    abstract::The luciferase gene expression of lipoplexes, a liposome containing luciferase plasmid (pCMVLuc), in HeLa cell lines, was investigated. Cationic liposomes were prepared by the chloroform film method with sonication. The lipoplex was formed by loading the liposome with pCMVLuc. The lipoplex with an optimal weight ratio...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982100802642457

    authors: Manosroi A,Thathang K,Manosroi J,Werner RG,Schubert R,Peschka-Süss R

    更新日期:2009-01-01 00:00:00

  • Proof of concept studies for siRNA delivery by nonionic surfactant vesicles: in vitro and in vivo evaluation of protein knockdown.

    abstract::RNA interference is an effective and naturally occurring post-transcriptional gene regulatory mechanism. This mechanism involves the degradation of a target messenger RNA (mRNA) through the introduction of short interfering RNA (siRNA) that is complementary to the target mRNA. The application of siRNA-based therapeuti...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982104.2018.1531424

    authors: Obeid MA,Dufès C,Somani S,Mullen AB,Tate RJ,Ferro VA

    更新日期:2019-09-01 00:00:00

  • Design and development of ethosomal transdermal drug delivery system of valsartan with preclinical assessment in Wistar albino rats.

    abstract::Valsartan (VLT) is a highly selective and orally active antihypertensive drug. However, its oral administration is associated with drawbacks like low bioavailability. The objective of this study was to design and develop a transdermal delivery system for VLT using ethosomal carriers to investigate their enhanced trans...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2012.753457

    authors: Bhosale SS,Avachat AM

    更新日期:2013-06-01 00:00:00

  • Antibodies to lipids and liposomes: immunology and safety.

    abstract::Naturally occurring antibodies to phospholipids and cholesterol are widespread; they occur commonly during the course of acute infections; they are not causally related to the anti-phospholipid syndrome; they have been associated with other clinical entities only as an epiphenomenon; and they have not been implicated ...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982100600848553

    authors: Alving CR

    更新日期:2006-01-01 00:00:00

  • Investigating superiority of novel bilosomes over niosomes in the transdermal delivery of diacerein: in vitro characterization, ex vivo permeation and in vivo skin deposition study.

    abstract::Skin is considered the most accessible organ of the body because of its underlying capillary network. However, stratum corneum (SC), the upper most layer of skin, represents major diffusional barrier for most drugs. Hence, the use of edge activators (EAs) in designing novel elastic vesicles is hypothesized to impart t...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982104.2018.1430831

    authors: Aziz DE,Abdelbary AA,Elassasy AI

    更新日期:2019-03-01 00:00:00

  • Induction of alloreactive cytotoxic T lymphocytes by intra-splenic immunization with allogeneic class I Major Histocompatibility Complex DNA and DC-chol cationic liposomes.

    abstract::A simple strategy for designing a cancer immunotherapeutic system involves modification of tumor cells from tumor-bearing animals in vivo in such a way that the host can evoke a specific immune response against them. We have expressed allogeneic class I major histocompatibility complex (MHC) molecules on tumor cells, ...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982109409018623

    authors: Hui KM,Sabapathy TK,Oei AA,Singhal A,Huang L

    更新日期:1994-01-01 00:00:00

  • Design and evaluation of proniosomes as a carrier for ocular delivery of lomefloxacin HCl.

    abstract::The current investigation aims to develop and evaluate novel ocular proniosomal gels of lomefloxacin HCl (LXN); in order to improve its ocular bioavailability for the management of bacterial conjunctivitis. Proniosomes were prepared using different types of nonionic surfactants solely and as mixtures with Span 60. The...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2016.1167737

    authors: Khalil RM,Abdelbary GA,Basha M,Awad GE,El-Hashemy HA

    更新日期:2017-06-01 00:00:00

  • Development of egg PC/cholesterol/α-tocopherol liposomes with ionic gradients to deliver ropivacaine.

    abstract:CONTEXT:Ropivacaine (RVC) is an aminoamide local anesthetic widely used in surgical procedures. Studies with RVC encapsulated in liposomes and complexed in cyclodextrins have shown good results, but in order to use RVC for lengthy procedures and during the postoperative period, a still more prolonged anesthetic effect ...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2015.1022555

    authors: da Silva CM,Fraceto LF,Franz-Montan M,Couto VM,Casadei BR,Cereda CM,de Paula E

    更新日期:2016-01-01 00:00:00

  • Efficacy study of vesicular gel containing methotrexate and menthol combination on parakeratotic rat skin model.

    abstract::Methotrexate (MTX) is indicated in the symptomatic control of severe, recalcitrant, and disabling psoriasis. The oral or parenteral route of administration causes systemic toxicity. The topical route of delivery, though, reduces systemic toxicity and has limited applicability due to restricted permeability. Liposomal ...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2010.492476

    authors: Nagle A,Goyal AK,Kesarla R,Murthy RR

    更新日期:2011-06-01 00:00:00

  • Optimizing long-circulating liposomes for delivery of simvastatin to C26 colon carcinoma cells.

    abstract::Simvastatin (SIM) is a lipophilic statin that has potential benefits for prevention and treatment of several types of malignancies. However, its low water solubility and the toxicity associated with administration of high doses recommend it for encapsulation in carriers able to deliver the therapeutic dose in the tumo...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2014.987787

    authors: Porfire A,Tomuta I,Muntean D,Luca L,Licarete E,Alupei MC,Achim M,Vlase L,Banciu M

    更新日期:2015-01-01 00:00:00

  • Development of liposomes loaded with anti-leishmanial drugs for the treatment of cutaneous leishmaniasis.

    abstract::Cutaneous leishmaniasis is caused by different species of Leishmania parasites and its available treatments have not yet provided a strong consistent result. The weak response of current chemotherapeutics is due to their deficient effects on stealth parasites inside macrophages, rapid clearance from the site of action...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2012.762519

    authors: Momeni A,Rasoolian M,Momeni A,Navaei A,Emami S,Shaker Z,Mohebali M,Khoshdel A

    更新日期:2013-06-01 00:00:00

  • Two-step impact of Amphotericin B (AmB) on lipid membranes: ESR experiment and computer simulations.

    abstract::In this study, the electron spin resonance (ESR) method was used to examine the effect of Amphotericin B (AmB) molecules on the fluidity of model membranes made of dipalmitoylphosphatidylcholine (DPPC). The changes occurring under increased AmB concentrations in the spectroscopic parameters of spin probes placed in li...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2013.814139

    authors: Man D,Olchawa R

    更新日期:2013-12-01 00:00:00

  • Effect of esters based on terpenoids and GABA on fluidity of phospholipid membranes.

    abstract::The influence of esters based on gamma-aminobutyric acid (GABA) and mono-/bicyclic terpenoids on membrane structure was investigated. The mechanism of action for terpenoid esters on phospholipids of artificial membranes and lipids isolated from the rat stratum corneum was studied by fluorescence and FT-IR spectroscopy...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982104.2018.1538238

    authors: Nesterkina M,Smola S,Kravchenko I

    更新日期:2019-09-01 00:00:00

  • Reducing liposome size with ultrasound: bimodal size distributions.

    abstract::Sonication is a simple method for reducing the size of liposomes. We report the size distributions of liposomes as a function of sonication time using three different techniques. Liposomes, mildly sonicated for just 30 sec, had bimodal distributions when surface-weighted with modes at about 140 and 750 nm. With extend...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982100500528842

    authors: Woodbury DJ,Richardson ES,Grigg AW,Welling RD,Knudson BH

    更新日期:2006-01-01 00:00:00

  • Co-delivery of gambogenic acid and VEGF-siRNA with anionic liposome and polyethylenimine complexes to HepG2 cells.

    abstract::Background and objective: The combination of two or more different mechanisms of drugs in the treatment of cancer has become one of the effective methods. The purpose of this study was to successfully prepare a non-viral delivery system that could efficiently co-delivery siRNA and gambogenic acid (GNA) to improve the ...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982104.2018.1473423

    authors: Yu Q,Zhang B,Zhou Y,Ge Q,Chang J,Chen Y,Zhang K,Peng D,Chen W

    更新日期:2019-12-01 00:00:00

  • Development and characterization of minoxidil-loaded liposomal system for delivery to pilosebaceous units.

    abstract::The current study aimed to deliver minoxidil (2,4-diamino-6-piperidinopyrimidine 3-oxide; MXD), a potent hypertrichotic agent, into the pilosebaceous units, exploring the potential of the liposomal system. MXD-loaded liposomes of different compositions were prepared by a thin-film hydration technique and subsequently ...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982100903161449

    authors: Jain B,Singh B,Katare OP,Vyas SP

    更新日期:2010-06-01 00:00:00

  • Delivery of fluorescent-labeled cyclodextrin by liposomes: role of transferrin modification and phosphatidylcholine composition.

    abstract::Drug-in-CD-in-liposome (DCL) systems which encapsulate the drug/CD inclusion complexes into inner aqueous phase of liposomes have been applied as a novel strategy to improve efficacy of lipophilic antitumor drugs. The aim of this work was to assess the role of transferrin (Tf) modification and phosphatidylcholine (PC)...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2016.1140184

    authors: Chen J,Yao J,Ma Z,Peng P,Lu S,Hu Y,Xu F,Yang Y,Yang X

    更新日期:2017-03-01 00:00:00

  • Liposome formation with wool lipid extracts rich in ceramides.

    abstract::Internal wool lipids (IWLs) are rich in cholesterol, free fatty acids, cholesteryl sulfate, and, mainly, ceramides. The repairing effect of these lipids structured as liposomes was demonstrated by reinforcing the skin-barrier integrity and increasing the water-holding capacity when applied onto the skin. This work was...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982100802538838

    authors: Ramírez R,Martí M,Cavaco-Paulo A,Silva R,de la Maza A,Parra JL,Coderch L

    更新日期:2009-01-01 00:00:00

  • Effect of sucrose as a lyoprotectant on the integrity of paclitaxel-loaded liposomes during lyophilization.

    abstract:CONTEXT:The stability of liposomes in the form of dispersion is a major concern due to drug leakage and fusion or aggregation. The stability can be improved by lyophilization, but the stress induced by the lyophilization process can affect the integrity of liposomes. OBJECTIVE:The objective of this study was to evalua...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2014.992023

    authors: Kannan V,Balabathula P,Thoma LA,Wood GC

    更新日期:2015-01-01 00:00:00

  • Doxorubicin and chloroquine coencapsulated liposomes: preparation and improved cytotoxicity on human breast cancer cells.

    abstract::Doxorubicin, as a widely used chemotherapeutic, always causes multidrug resistance in human cancer cells. To circumvent drug resistance, we developed a novel formulation where doxorubicin hydrochloride (DOX) and chloroquine phosphate (CQ) were simultaneously loaded into liposomes by a pH-gradient method where CQ playe...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982104.2012.684150

    authors: Qiu L,Yao M,Gao M,Zhao Q

    更新日期:2012-09-01 00:00:00

  • PEGylated niosomes-mediated drug delivery systems for Paeonol: preparation, pharmacokinetics studies and synergistic anti-tumor effects with 5-FU.

    abstract::This work describes the preparation of a PEGylated niosomes-mediated drug delivery systems for Paeonol, thereby improving the bioavailability and chemical stability of Paeonol, prolonging its cellular uptake and enhancing its synergistic anti-cancer effects with 5-Fu. PEGylated niosomes, which are prepared from biocom...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982104.2016.1191021

    authors: He RX,Ye X,Li R,Chen W,Ge T,Huang TQ,Nie XJ,Chen HJ,Peng DY,Chen WD

    更新日期:2017-06-01 00:00:00

  • HER2-specific affibody-conjugated thermosensitive liposomes (Affisomes) for improved delivery of anticancer agents.

    abstract::Thermosensitive liposomes are attractive vehicles for the delivery and release of drugs to tumors. To improvethe targeting efficacy for breast cancer treatment, an 8.3-kDa HER2-specific Affibody molecule (Z(HER2:342)-Cys) was conjugated to the surface of liposomes. The effects of this modification on physical characte...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982100802457377

    authors: Puri A,Kramer-Marek G,Campbell-Massa R,Yavlovich A,Tele SC,Lee SB,Clogston JD,Patri AK,Blumenthal R,Capala J

    更新日期:2008-01-01 00:00:00

  • Preparation and characterization of zedoary turmeric oil-loaded insulin-modified sterically stabilized liposomes.

    abstract::The poor selectivity of anticancer drugs often leads to their multiplicate dose-limiting toxicities in humans, which severely restricts their clinical application. In this study, a novel liposomal formulation of zedoary turmeric oil (ZTO) targeting the insulin receptor (IR) was prepared by covalently conjugating insul...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.3109/08982100903015017

    authors: Yang Z,Fu D,Zhu L,Yang M,Cheng Q

    更新日期:2010-03-01 00:00:00

  • Sensitization of multidrug-resistant malignant cells by liposomes co-encapsulating doxorubicin and chloroquine through autophagic inhibition.

    abstract::Adenosine triphosphate (ATP)-binding cassette (ABC) transporters play a key role in the development of multidrug resistance (MDR) in cancer cells. P-glycoprotein (P-gp) and multidrug resistance-associated protein 1 (MRP1) are important proteins in this superfamily which are widely expressed on the membranes of multidr...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982104.2016.1185731

    authors: Gao M,Xu Y,Qiu L

    更新日期:2017-06-01 00:00:00

  • Arsonoliposomes: effect of lipid composition on their stability and morphology.

    abstract::The influence of the lipid composition of arsonoliposomes on their membrane integrity was investigated to evaluate whether it is possible to combine their action with drugs that can be encapsulated in their aqueous interior. This was investigated by measuring the retention of vesicle-encapsulated calcein (100 mM) duri...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1080/08982100500364263

    authors: Piperoudi S,Ioannou PV,Frederik P,Antimisiaris SG

    更新日期:2005-01-01 00:00:00

  • Safety of archaeosome adjuvants evaluated in a mouse model.

    abstract::Archaeosomes, liposomes prepared from the polar ether lipids extracted from Archaea, demonstrate great potential as immunomodulating carriers of soluble antigens, promoting humoral and cell mediated immunity in the vaccinated host. The safety of unilamellar archaeosomes prepared from the total polar lipids (TPL) of Ha...

    journal_title:Journal of liposome research

    pub_type: 杂志文章

    doi:10.1081/lpr-120016712

    authors: Patel GB,Omri A,Deschatelets L,Sprott GD

    更新日期:2002-11-01 00:00:00

  • Animal models of complement-mediated hypersensitivity reactions to liposomes and other lipid-based nanoparticles.

    abstract::Intravenous injection of some liposomal drugs, diagnostic agents, micelles and other lipid-based nanoparticles can cause acute hypersensitivity reactions (HSRs) in a high percentage (up to 45%) of patients, with hemodynamic, respiratory and cutaneous manifestations. The phenomenon can be explained with activation of t...

    journal_title:Journal of liposome research

    pub_type: 杂志文章,评审

    doi:10.1080/08982100701375118

    authors: Szebeni J,Alving CR,Rosivall L,Bünger R,Baranyi L,Bedöcs P,Tóth M,Barenholz Y

    更新日期:2007-01-01 00:00:00