Abstract:
:The aim of this study was to investigate the application of ultrasonic gas-filled liposomes in enhancing transfer for breast cancer-related antisense oligonucleotides in vitro. An antisense oligodeoxynucleotide (AS-ODN) sequence, HA2741, modified with luciferase reporter plasmid, was used in evaluating the enhancing effect of gas-filled liposomes for gene transfer in breast cancer cells. Some important factors on HA 2741 transfection efficiency, such as wave intensity, ultrasound duration, gas-filled liposome concentration, and HA2741 concentration, were tested, respectively. Transfection efficiency was detected by fluorescence microscopy. Cell viability was verified by propidium iodide assay. Reverse-transcriptase polymerase chain reaction and immunocytochemistry were used to detect the inhibitory effect of HA2741 on HER-2 expression. All the four factors (wave intensity, ultrasound duration, gas-filled liposome concentration, and HA2741 concentration) showed a positive effect on AS-ODN transfection efficiency. However, these factors had a negative effect on cell viability. Considering all the factors investigated, the maximum transfection efficiency with minimum cell viability achieved under 2% gas-filled liposome mixed with 80 nmol/L HA2741 for 30-second ultrasound exposure at -3.0 dB wave intensity, which gave an overall transfection efficiency exceeding 90% and a cell viability near 90%. Under controlled conditions, ultrasound-mediated AS-ODN transfer, enhanced by gas-filled liposomes, may represent an effective, safe avenue for cancer-related gene delivery.
journal_name
J Liposome Resjournal_title
Journal of liposome researchauthors
Luo YK,Zhao YZ,Lu CT,Tang J,Li XKdoi
10.1080/03639040802509868subject
Has Abstractpub_date
2008-01-01 00:00:00pages
341-51issue
4eissn
0898-2104issn
1532-2394pii
905063038journal_volume
18pub_type
杂志文章abstract::The present study is envisaged to develop nanoethosomal formulation for enhanced topical delivery of amphotericin B (AmB) for the treatment of cutaneous fungal infections. AmB encapsulated nanoethosomes were prepared using mechanical dispersion method in a strength of 0.1% w/w similar to the strength of marketed topic...
journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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pub_type: 杂志文章,评审
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