Abstract:
:The aim of present study was to develop conventional and PEGylated (long circulating), liposomes containing anastrozole (ANS) for effective treatment of breast cancer. ANS is a third-generation non-steroidal aromatase inhibitor of the triazole class used for the treatment of advanced and late-stage breast cancer in post-menopausal women. Under such disease conditions the median duration of therapy should be prolonged until tumor regression ends (>31 months). Liposomes were prepared by the thin film hydration method by using ANS and various lipids such as soyaphosphatidyl choline, cholesterol and methoxy polyethylene glycol distearoyl ethanolamine in different concentration ratios and evaluated for physical characteristics, in vitro drug release and stability. Optimized formulations of liposome were studied for in vitro cytotoxic activity against the BT-549 and MCF-7 cell lines and in vivo behavior in Wistar rats. Preformulation studies, both Fourier transform infrared study and differential scanning calorimetry analysis showed no interaction between the drug and the excipients used in the formulations. The optimized formulations AL-07 and AL-09 liposomes showed encapsulation efficiencies in the range 65.12 ± 1.05% to 69.85 ± 3.2% with desired mean particle size distribution of 101.1 ± 5.9 and 120.2 ± 2.8 nm and zeta potentials of -43.7 ± 4.7 and -62.9 ± 3.5 mV. All the optimized formulations followed Higuchi-matrix release kinetics and when plotted in accordance with the Korsemeyer-Peppas method, the n-value 0.5 < n < 1.0 suggests an anomalous (non-Fickian) transport. Likewise, the PEGylated liposomes showed greater tumor growth inhibition on BT-549 and MCF-7 cell lines from in vitro cytotoxicity studies (p < 0.05). Pharmacokinetic study of conventional and PEGylated liposomes in Wistar rats demonstrated a 3.33- and 20.28-fold increase in AUC(0-∞) values when compared to pure drug (p < 0.001). Among the formulations, PEGylated liposomes showed encouraging results by way of their long circulation and sustained delivery properties for effective treatment of breast cancer.
journal_name
J Liposome Resjournal_title
Journal of liposome researchauthors
Shavi GV,Sreenivasa Reddy M,Raghavendra R,Nayak UY,Kumar AR,Deshpande PB,Udupa N,Behl G,Dave V,Kushwaha Kdoi
10.3109/08982104.2015.1029493subject
Has Abstractpub_date
2016-01-01 00:00:00pages
28-46issue
1eissn
0898-2104issn
1532-2394journal_volume
26pub_type
杂志文章abstract::Propylthiouracil, a lyophobic drug with an antiproliferative activity, was formulated into niosomes using various classes of nonionic surfactants. Feasibility of vesicle formation by the sonication method was evaluated. Size and size distribution was measured by laser diffraction. Entrapment and drug release over 24 h...
journal_title:Journal of liposome research
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doi:10.1080/08982100600992542
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
pub_type: 杂志文章
doi:10.3109/08982100902788408
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
pub_type: 杂志文章
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journal_title:Journal of liposome research
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journal_title:Journal of liposome research
pub_type: 杂志文章,评审
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更新日期:2007-01-01 00:00:00
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journal_title:Journal of liposome research
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更新日期:2016-01-01 00:00:00
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journal_title:Journal of liposome research
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更新日期:2002-11-01 00:00:00
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更新日期:2019-09-01 00:00:00
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journal_title:Journal of liposome research
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更新日期:2012-09-01 00:00:00
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journal_title:Journal of liposome research
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更新日期:2019-09-01 00:00:00