Abstract:
Background:Sepsis-induced myocardial dysfunction is a severe clinical problem. Recent studies have indicated that autophagy and myocardial energy depletion play a major role in myocardial dysfunction during sepsis, a mechanistic target of rapamycin (mTOR) as a master sensor of energy status and autophagy mediator; however, there are little data describing its role during sepsis in the heart. Methods:Cecal ligation and puncture (CLP) or sham operation (SHAM) was performed in rats. After treatment, pathological changes were determined by H&E staining, cardiac functions by echocardiography, the distribution of microtubule-associated protein light chain 3 (LC-3) type II and hypoxia-inducible factor 1α (HIF-1a) by immunohistochemical staining, and autophagic vacuoles by transmission electron microscopy. Moreover, the mTOR signaling pathway and LC3II, p62, and HIF-1a expression were measured by western blotting. Results:Rapamycin alleviated the pathological damage of myocardial tissue, attenuated cardiac dysfunction (left ventricular ejection fraction (LVEF), p < 0.05; fractional shortening (FS), p < 0.05), and reduced HIF-1a expression (p < 0.05). Expectedly, rapamycin decreased the activity of the mTOR pathway in both sham-operated rats (p < 0.0001) and CLP rats (p < 0.01). Interestingly, we also found inhibition of the mTOR pathway in CLP rats compared with sham-operated rats; phosphorylation of both mTOR (p < 0.001) and pS6K1 (p < 0.01) was significantly suppressed following CLP challenge. Furthermore, autophagic processes were elevated by CLP; the ratio of LC3II/LC3I (p < 0.05) was increased while p62 expression (p < 0.001) was decreased significantly; there were also more autophagic vacuoles in CLP rats; and rapamycin could further elevate the autophagic processes compared with CLP rats (LC3II/LC3I, p < 0.05; P62, p < 0.05). Conclusion:Inhibition of the mTOR pathway has cardioprotective effects on myocardial dysfunction during sepsis induced by CLP, which is partly mediated through autophagy.
journal_name
Mediators Inflammjournal_title
Mediators of inflammationauthors
Han W,Wang H,Su L,Long Y,Cui N,Liu Ddoi
10.1155/2018/4798209subject
Has Abstractpub_date
2018-06-28 00:00:00pages
4798209eissn
0962-9351issn
1466-1861journal_volume
2018pub_type
杂志文章abstract::Aim. Inflammation is important in heart failure (HF). The role of the immune receptor toll-like receptor 9 (TLR9) in HF is not understood and not investigated in diastolic HF. We investigated the role of TLR9 in a murine diastolic HF model caused by cardiomyocyte SERCA2a excision. Methods and Results. We crossed SERCA...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2017/9450439
更新日期:2017-01-01 00:00:00
abstract::Dysfunction of the blood-brain barrier (BBB) is a primary characteristic of experimental autoimmune encephalomyelitis (EAE), an experimental model of multiple sclerosis (MS). Matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae Flave, has been recently found to suppress clinical EAE and CNS inf...
journal_title:Mediators of inflammation
pub_type: 杂志文章
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abstract:OBJECTIVE:The proapoptotic protein, granzyme B (GZB), was identified as a contributor to the atherosclerotic plaque instability and recently as inflammatory activator. We studied the release kinetics of GZB and other markers of inflammation such as high sensitivity C reactive protein (hsCRP), interleukin 18 (IL-18), an...
journal_title:Mediators of inflammation
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doi:10.1155/2013/343268
更新日期:2013-01-01 00:00:00
abstract::Cardiovascular disease linked to atherosclerosis is the leading cause of death worldwide. Atherosclerosis is mainly linked to dysfunction in vascular endothelial cells and subendothelial accumulation of oxidized forms of LDL. In the present study, we investigated the role of myeloperoxidase oxidized LDL (Mox-LDL) in e...
journal_title:Mediators of inflammation
pub_type: 杂志文章
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abstract:BACKGROUND:Production of antibodies that are specific for allergens is an important pathological process in inflammatory allergic diseases. These contain the antibodies against antigens of Candida albicans, one of the normal microbial flora in an intestinal tract. We studied the effects of the prednisolone administrati...
journal_title:Mediators of inflammation
pub_type: 杂志文章
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abstract::Inflammatory bowel disease (IBD) describes different illnesses characterized by chronic inflammation of the gastrointestinal tract. Although the pathogenic mechanisms leading to IBD are poorly understood, immune system disturbances likely underlie its development. Sphingolipids (SLs) have been identified as important ...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
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journal_title:Mediators of inflammation
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journal_title:Mediators of inflammation
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journal_title:Mediators of inflammation
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journal_title:Mediators of inflammation
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更新日期:2014-01-01 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2009/287387
更新日期:2009-01-01 00:00:00
abstract::Crohn's disease (CD) is a multifactorial chronic inflammatory bowel disease of unknown cause. The aim of the present study was to explore if mRNA over-expression of SSTR5 and CCR7 found in CD patients could be correlated to respective protein expression. When compared to healthy donors, SSTR5 was over-expressed 417 +/...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2009/285812
更新日期:2009-01-01 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
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更新日期:2019-01-08 00:00:00
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journal_title:Mediators of inflammation
pub_type: 已发布勘误
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更新日期:2020-06-17 00:00:00
abstract::Cytokines and chemokines are proteins that coordinate the immune response throughout the body. The dysregulation of cytokines and chemokines is a central feature in the development of neuroinflammation, neurodegeneration, and demyelination both in the central and peripheral nervous systems and in conditions of neuropa...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2013/480739
更新日期:2013-01-01 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2015/132458
更新日期:2015-01-01 00:00:00
abstract::Podocyte injury critically contributes to the pathogenesis of obesity-related glomerulopathy (ORG). Recently, lipid accumulation and inflammatory responses have been found to be involved in podocyte injury. This study is to explore their role and relationship in podocyte injury of ORG. In animal experiments, the ORG m...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2019/3172647
更新日期:2019-04-11 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/09629350020002877
更新日期:2000-01-01 00:00:00
abstract:STUDY OBJECTIVES:To assess serum amyloid alpha (SAA) pleural fluid levels in parapneumonic effusion (PPE) and to investigate SAA diagnostic performance in PPE diagnosis and outcome. METHODS:We studied prospectively 57 consecutive patients with PPE (empyema (EMP), complicated (CPE), and uncomplicated parapneumonic effu...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2011/237638
更新日期:2011-01-01 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
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更新日期:2013-01-01 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2012/912595
更新日期:2012-01-01 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2013/928315
更新日期:2013-01-01 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2016/4158287
更新日期:2016-01-01 00:00:00
abstract::To test the statistical association of the CRP and SAA1 locus variants with their corresponding circulating levels and metabolic and inflammatory biomarker levels by using mediation analysis, a sample population of 599 Taiwanese subjects was enrolled and five CRP and four SAA1 variants were genotyped. Correlation anal...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2016/5830361
更新日期:2016-01-01 00:00:00
abstract::B-1a cells are distinguishable from conventional B cells, which are designated B-2 cells, on the basis of their developmental origin, surface marker expression, and functions. In addition to the unique expression of the CD5 antigen, B-1a cells are characterized by the expression level of CD23. Although B-1a cells are ...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2018/9289417
更新日期:2018-12-23 00:00:00
abstract::The effect of exogenous RNA on many cellular functions has been studied in a variety of eukaryotic cells but there are few reports on macrophages. In the present study, it is demonstrated that cytoplasmatic RNA extracted from rat macrophages stimulated with Escherichia coli lipopolysaccharide (LPS), referred to as L-R...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/S0962935193000626
更新日期:1993-01-01 00:00:00
abstract::Obesity is marked by chronic, low-grade inflammation. Here, we examined whether intrinsic differences between white and brown adipocytes influence the inflammatory status of macrophages. White and brown adipocytes were characterized by transcriptional regulation of UCP-1, PGC1α, PGC1β, and CIDEA and their level of IL-...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2017/9067049
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journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2016/8769347
更新日期:2016-01-01 00:00:00
abstract::High-fructose diet induced changes in gut microbiota structure and function, which have been linked to inflammatory response. However, the effect of small or appropriate doses of fructose on gut microbiota and inflammatory cytokines is not fully understood. Hence, the abundance changes of gut microbiota in fructose-tr...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2020/6672636
更新日期:2020-11-30 00:00:00
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journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/MI/2006/12919
更新日期:2006-01-01 00:00:00