Abstract:
BACKGROUND:Ubiquitous polycyclic aromatic hydrocarbons (PAHs) have been shown to alter gene expression patterns and elevate micronuclei (MN) frequency, but the underlying mechanisms are largely unknown. MicroRNAs (miRNAs) are key gene regulators that may be influenced by PAH exposures and mediate their effects on MN frequency. OBJECTIVES:We sought to identify PAH-associated miRNAs and evaluate their associations with MN frequency. METHODS:We performed a two-stage study in healthy male coke oven workers to identify miRNAs associated with PAH exposures quantified using urinary monohydroxy-PAHs and plasma benzo[a]pyrene-r-7,t-8,c-10-tetrahydrotetrol-albumin (BPDE-Alb) adducts. In the discovery stage, we used Solexa sequencing to test differences in miRNA expression profiles between pooled plasma samples from 20 exposed workers and 20 controls. We then validated associations with eight selected miRNAs in 365 workers. We further evaluated associations between the PAH-associated miRNAs and MN frequency. RESULTS:In the discovery stage, miRNA expression profiles differed between the exposed and control groups, with 68 miRNAs significantly down-regulated [fold change (FC) ≤ -5] and 3 miRNAs mildly up-regulated (+2 ≤ FC < +5) in the exposed group. In the validation analysis, urinary 4-hydroxyphenanthrene and/or plasma BPDE-Alb adducts were associated with lower miR-24-3p, miR-27a-3p, miR-142-5p, and miR-28-5p expression (p < 0.030). Urinary 1-hydroxynaphthalene, 2-hydroxynaphthalene, 2-hydroxyphenanthrene, and the sum of monohydroxy-PAHs were associated with higher miR-150-5p expression (p < 0.030). These miRNAs were associated with higher MN frequency (p < 0.005), with stronger associations in drinkers (pinteraction < 0.015). CONCLUSIONS:Associations of PAH exposures with miRNA expression, and of miRNA expression with MN frequency, suggest potential mechanisms of adverse effects of PAHs that are worthy of further investigation.
journal_name
Environ Health Perspectjournal_title
Environmental health perspectivesauthors
Deng Q,Huang S,Zhang X,Zhang W,Feng J,Wang T,Hu D,Guan L,Li J,Dai X,Deng H,Zhang X,Wu Tdoi
10.1289/ehp.1307080subject
Has Abstractpub_date
2014-07-01 00:00:00pages
719-25issue
7eissn
0091-6765issn
1552-9924journal_volume
122pub_type
杂志文章abstract::The N-oxidation of carcinogenic arylamines to form N-hydroxy arylamines has long been regarded as a necessary metabolic step for conversion to proximate carcinogenic derivatives. In contrast, arylamine ring-oxidation has been generally considered to be an important detoxification mechanism. Both enzymatic reactions ar...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.9087233
更新日期:1990-07-01 00:00:00
abstract::Traditional models of toxicity have relied on dissecting chemical action into pharmacokinetic and pharmacodynamic processes. However, the integration of genomic information with toxicology will enhance our basic understanding of these processes and significantly change the way we apply toxicological information to ris...
journal_title:Environmental health perspectives
pub_type: 杂志文章,评审
doi:10.1289/ehp.02110s6919
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:Cancer may be a stem cell (SC)-based disease involving formation of cancer SCs (CSCs) potentially arising from transformation of normal SCs. Cadmium has been linked to human pancreatic cancer. OBJECTIVE:We studied cadmium exposure of human pancreatic ductal epithelial (HPDE) cells and whether SCs may be tar...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.1205082
更新日期:2012-09-01 00:00:00
abstract::Children may be more susceptible to exposures to environmental toxins than adults and may be more vulnerable to their effects. Because of this, the health care community and those responsible for children need to be alert to possible environmental factors in identifying and responding to the health problems of childre...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.95103s613
更新日期:1995-09-01 00:00:00
abstract::New technologies are providing innovative ways to reduce sound levels in many areas. Aircraft engineers are finding ways to reduce the noise produced by jet engines, while road builders are using rubber-enhanced pavement to quiet highway noise. Indoor acoustics are benefiting from materials that transform sound waves ...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.113-a46
更新日期:2005-01-01 00:00:00
abstract::Studies conducted by the National Toxicology Program on the chronic toxicity of benzene indicated that B6C3F1 mice were more sensitive to the carcinogenic effects of benzene than were F344 rats. A physiological model was developed to describe the uptake and metabolism of benzene in rats and mice. Our objective was to ...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.898243
更新日期:1989-07-01 00:00:00
abstract::Enhanced transport of trace metal in porous media can occur in the presence of a ligand or "carrier" that has a high affinity for binding the pollutant, is dispersed and mobile in the soil environment, is recalcitrant with respect to microbial degradation, and is acceptable to the public. These aspects of the facilita...
journal_title:Environmental health perspectives
pub_type: 杂志文章,评审
doi:10.1289/ehp.95103s153
更新日期:1995-02-01 00:00:00
abstract::A major goal of studying farmworkers is to better understand how their work environment, including exposure to pesticides, affects their health. Although a number of health conditions have been associated with pesticide exposure, clear linkages have yet to be made between exposure and health effects except in cases of...
journal_title:Environmental health perspectives
pub_type: 杂志文章,评审
doi:10.1289/ehp.8526
更新日期:2006-06-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.803491
更新日期:1980-02-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.1408535
更新日期:2015-05-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章,评审
doi:10.1289/ehp.97105s2521
更新日期:1997-03-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.1206211
更新日期:2013-10-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/EHP1673
更新日期:2017-11-07 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.9086107
更新日期:1990-06-01 00:00:00
abstract:BACKGROUND:Although patients with heart failure (HF) have been identified as particularly susceptible to the acute effects of air pollution, the effects of long-term exposure to air pollution on patients with this increasingly prevalent disease are largely unknown. OBJECTIVE:This study was designed to examine the mort...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.10918
更新日期:2008-04-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.5775
更新日期:2003-04-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.866861
更新日期:1986-09-01 00:00:00
abstract:BACKGROUND:Phthalates comprise a large class of chemicals used in a variety of consumer products. Several have anti-androgenic properties, and in rodents prenatal exposure has been associated with reduced anogenital distance (AGD)-the distance from the anus to the genitals in male offspring. Few human studies have been...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.1509870
更新日期:2016-07-01 00:00:00
abstract::In rats, a single administration of acrylonitrile (vinyl cyanide) produces a rapidly occurring bilateral adrenal apoplexy. Structure-activity studies have shown that a close derivative, propionitrile (ethyl cyanide), causes duodenal ulcer without markedly affecting the adrenal glands. Prolonging the two-carbon chain o...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.7511135
更新日期:1975-06-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/EHP6652
更新日期:2020-11-01 00:00:00
abstract::We investigated the metabolic fate of a low dose (25 micro g/kg) of bisphenol A [2,2-bis(4-hydroxy-phenyl)propane] (BPA) injected subcutaneously in CD1 pregnant mice using a tritium-labeled molecule. Analytic methods were developed to allow a radio-chromatographic profiling of BPA residues in excreta and tissues, as w...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.5603
更新日期:2003-03-01 00:00:00
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journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.7720257
更新日期:1977-10-01 00:00:00
abstract:BACKGROUND:Cumulative lead exposure is associated with a widened pulse pressure (PP; the -difference between systolic and diastolic blood pressure), a marker of arterial stiffness and a predictor of cardiovascular disease. Polymorphisms in the hemochromatosis gene (HFE) have been shown to modify the impact of cumulativ...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.1002251
更新日期:2010-09-01 00:00:00
abstract::Possible effects of Chernobyl fallout on outcome of pregnancy in Finland were evaluated in a nationwide follow-up study. The outcomes were the rate of live births and stillbirths, pregnancy loss, and induced abortions by municipality. Exposure was assessed based on nationwide surveys of radiation dose rate from the Ch...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.01109179
更新日期:2001-02-01 00:00:00
abstract::Inorganic particulate matter in foods and drugs is discussed from the standpoint of determination by optical microscopy, source, and regulatory significance. Some particulate matter may be generated as extraneous material and traced to specific operational practices during processing and production with excessive leve...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.749183
更新日期:1974-12-01 00:00:00
abstract::Asthma is the most common chronic illness of childhood and its prevalence is increasing, causing much concern for identification of risk factors such as air pollution. We previously conducted a study showing a relationship between asthma visits in all persons < 65 years of age to emergency departments (EDs) and air po...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.99107489
更新日期:1999-06-01 00:00:00
abstract::Groundwater contamination is a serious problem that is growing in the United States, but its true extent is not known and it is difficult to determine because of the complexities of contaminants, their transformation, and fate in groundwater systems. It is also difficult to predict their movement in groundwater. Since...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.9086239
更新日期:1990-06-01 00:00:00
abstract::A brief history of the development of the field of aquatic toxicology is provided. In order to provide a perspective on the state-of-the-art in aquatic toxicology relative to classical toxicology, the two fields are compared from the standpoint of the type of scientist practicing each field, the respective objectives ...
journal_title:Environmental health perspectives
pub_type: 历史文章,杂志文章
doi:10.1289/ehp.8034159
更新日期:1980-02-01 00:00:00
abstract::High-performance liquid chromatography (HPLC) methods are described which permit the rapid isolation of multiple target macromolecules from the tissues of animals exposed to chemical mutagens. DNA and selected chromosomal proteins are isolated in a simple two step separation scheme. Isolated nuclei are dissolved in 3 ...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.856273
更新日期:1985-10-01 00:00:00
abstract::This study constitutes one part of a program for assessing the impact of coal-fired power plants on the surrounding communities. A questionnaire was mailed to a total of 12,000 subjects living in six areas with coal-fired plants and in matched reference areas. The participation rate was 77.3%. In one coal-fired plant/...
journal_title:Environmental health perspectives
pub_type: 杂志文章
doi:10.1289/ehp.8670239
更新日期:1986-12-01 00:00:00