Abstract:
:Surgical nerve damage due to difficulty with identification remains a major risk for postsurgical complications and decreased quality of life. Fluorescence-guided surgery offers a means to specifically highlight tissues of interest such as nerves and a number of fluorescence-guided surgical systems are in clinical trial or are approved for clinical use. However, no clinically approved nerve-specific fluorophores exist. In addition, many preclinical nerve-specific fluorophores tend to accumulate in adipose tissue due to the molecular composition similarities between the two tissues, making it challenging to generate a specific nerve signal. To alleviate this difficulty, we have synthesized a library of oxazine fluorophores based on the Nile Red scaffold, with the goal of strong adipose specificity without nerve uptake to facilitate ratiometric imaging. The library was screened for tissue specificity ex vivo and in vivo, enabling quantification of adipose-, nerve- and muscle-specific uptake as well as selection of the best candidate for adipose selectivity without nerve signal. We showed our selected Nile Red fluorophore improved nerve contrast using ratiometric imaging, especially nerve-to-adipose contrast as compared to the parent Nile Red compound or nerve-specific imaging alone. This adipose-specific Nile Red derivative could be used in future fluorescence-guided surgery applications where adipose- or nerve-specific contrast is required.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Korber J,Barth C,Gibbs Sdoi
10.1117/1.JBO.23.7.076002subject
Has Abstractpub_date
2018-07-01 00:00:00pages
1-13issue
7eissn
1083-3668issn
1560-2281pii
JBO-180172Rjournal_volume
23pub_type
杂志文章abstract::Optical coherence tomography (OCT)-based elasticity imaging can map soft tissue elasticity based on speckle-tracking of elastic wave propagation using highly sensitive phase measurements of OCT signals. Using a fixed elastic wave source and moving detection, current imaging sequences have difficulty in reconstructing ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.11.116006
更新日期:2016-11-01 00:00:00
abstract::We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm(2), 75 ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1560011
更新日期:2003-04-01 00:00:00
abstract::Within the framework of further development of unified Monte Carlo code for the needs of biomedical optics and biophotonics, we present an approach for modeling of coherent polarized light propagation in highly scattering turbid media, such as biological tissues. The temporal coherence of light, linear and circular po...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.2.025005
更新日期:2014-02-01 00:00:00
abstract::SpineLab is a software tool developed for reconstructing neuronal feature skeletons from three-dimensional single- or multi-photon image stacks. These images often suffer from limited resolution and a low signal-to-noise ratio, making the extraction of morphometric information difficult. To overcome this limitation, w...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.076007
更新日期:2012-07-01 00:00:00
abstract::We have applied wide-field digital interferometry (WFDI) to examine the morphology and dynamics of live red blood cells (RBCs) from individuals who suffer from sickle cell anemia (SCA), a genetic disorder that affects the structure and mechanical properties of RBCs. WFDI is a noncontact, label-free optical microscopy ...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3556717
更新日期:2011-03-01 00:00:00
abstract::A Monte Carlo algorithm is applied to simulate the measurements of a sample with glass slides sandwiched between the double integrating sphere (DIS) setup. The effects caused by various parameters, such as the sample port of integrating sphere, thicknesses, and optical properties of the sample, on light losses and opt...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2815691
更新日期:2007-11-01 00:00:00
abstract::Intraductal carcinoma is a precancerous lesion of the breast and the immediate precursor of invasive ductal carcinoma. Multiphoton microscopy (MPM) was used to monitor the progression from intraductal carcinoma to invasive ductal carcinoma, which can improve early detection of precursor lesions and halt progression to...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.20.9.096007
更新日期:2015-09-01 00:00:00
abstract::Characterization and three-dimensional (3-D) localization of human cancerous prostate tissue embedded in normal prostate tissue were demonstrated using backscattering scanning polarization imaging and an inverse imaging reconstruction algorithm, optical tomography using independent component analysis (OPTICA). Two-dim...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.8.081419
更新日期:2012-08-01 00:00:00
abstract::Oral cancer is one of the most common cancers worldwide. One-fifth of the world's oral cancer subjects are from India and other South Asian countries. The present Raman mapping study was carried out to understand biochemical variations in normal and malignant oral buccal mucosa. Data were acquired using WITec alpha 30...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.12.126005
更新日期:2014-12-01 00:00:00
abstract::Fiber-guided ablation of soft tissue with pulsed holmium and thulium lasers was investigated for intraluminal incisions. A bare fiber/tissue-contact application system with a nearly tangential irradiation geometry was first used in vitro on porcine ureter tissue. The efficiency and precision of the method was analyzed...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.429865
更新日期:1998-01-01 00:00:00
abstract::For clinical optoacoustic imaging, linear probes are preferably used because they allow versatile imaging of the human body with real-time display and free-hand probe guidance. The two-dimensional (2-D) optoacoustic image obtained with this type of probe is generally interpreted as a 2-D cross-section of the tissue ju...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.2.026011
更新日期:2013-02-01 00:00:00
abstract::Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure ...
journal_title:Journal of biomedical optics
pub_type: 临床试验,杂志文章
doi:10.1117/1.1695562
更新日期:2004-05-01 00:00:00
abstract::We have combined a confocal laser scanning head modified for TPE (two-photon excitation) microscopy with some spectroscopic modules to study single molecules and molecular aggregates. The behavior of the TPE microscope unit has been characterized by means of point spread function measurements and of the demonstration ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.1382809
更新日期:2001-07-01 00:00:00
abstract::We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.7.075008
更新日期:2012-07-01 00:00:00
abstract::Optical spectroscopy has shown potential as a real-time, in vivo, diagnostic tool for identifying neoplasia during endoscopy. We present the development of a diagnostic algorithm to classify elastic-scattering spectroscopy (ESS) spectra as either neoplastic or non-neoplastic. The algorithm is based on pattern recognit...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3592488
更新日期:2011-06-01 00:00:00
abstract:SIGNIFICANCE:Adaptive optics optical coherence tomography (AO-OCT) technology enables non-invasive, high-resolution three-dimensional (3D) imaging of the retina and promises earlier detection of ocular disease. However, AO-OCT data are corrupted by eye-movement artifacts that must be removed in post-processing, a proce...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.26.1.016001
更新日期:2021-01-01 00:00:00
abstract::Under ultraviolet and visible light excitation, melanin is essentially a nonfluorescent substance. This work reports our study on near-infrared (NIR) fluorescence properties of melanins, and explores potential applications of NIR fluorescence techniques for evaluating skin disorders involving melanin. The NIR fluoresc...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2204007
更新日期:2006-05-01 00:00:00
abstract::Measuring the elasticity distribution inside the human body is of great interest because elastic abnormalities can serve as indicators of several diseases. We present a method for mapping elasticity inside soft tissues by imaging surface acoustic waves (SAWs) with digital holographic interferometry. With this method, ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3646211
更新日期:2011-11-01 00:00:00
abstract::Lihong V. Wang summarizes his tenure as Editor-in-Chief of the Journal of Biomedical Optics and introduces his successor, Brian Pogue, who will assume the role in January 2018. ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.22.10.100101
更新日期:2017-10-04 00:00:00
abstract::Clustering analysis (CA) and principal component analysis (PCA) were applied to dynamic Cerenkov luminescence images (dCLI). In order to investigate the performances of the proposed approaches, two distinct dynamic data sets obtained by injecting mice with (32)P-ATP and (18)F-FDG were acquired using the IVIS 200 optic...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3663442
更新日期:2011-12-01 00:00:00
abstract::Suture ligation of blood vessels during surgery can be time-consuming and skill-intensive. Energy-based, electrosurgical, and ultrasonic devices have recently replaced the use of sutures and mechanical clips (which leave foreign objects in the body) for many surgical procedures, providing rapid hemostasis during surge...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.18.5.058001
更新日期:2013-05-01 00:00:00
abstract::We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) a...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3533310
更新日期:2011-02-01 00:00:00
abstract::We report the design of a novel laser line-triangulation laryngoscope for the quantitative visualization of the three-dimensional movements of human vocal folds during phonation. This is the first successful in vivo recording of the three-dimensional movements of human vocal folds in absolute values. Triangulation ima...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3041164
更新日期:2008-11-01 00:00:00
abstract::The scattering of polarized light from a two layer scattering medium is investigated using Monte Carlo simulations. First order and normalized second order moments are used to analyze the spatial properties of the emerging light in different polarization states. Linearly and circularly polarized illumination is used t...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1483316
更新日期:2002-07-01 00:00:00
abstract::We investigate the feasibility of using diode laser gas spectroscopy for sinusitis diagnostics. We simulate light propagation using the Monte Carlo concept, as implemented by the Advanced Systems Analysis Program (ASAP) software. Simulations and experimental data are compared for a model based on two scattering bodies...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2777179
更新日期:2007-09-01 00:00:00
abstract::Photoacoustic imaging has become an emerging tool for theranostic applications. Not only does it help in journal_title:Journal of biomedical optics pub_type: 杂志文章 doi:10.1117/1.JBO.22.4.041007 更新日期:2017-04-01 00:00:00
abstract::Microscopic visualization of islets of Langerhans under normal and diabetic conditions is essential for understanding the pathophysiology of the disease. The intrinsic opacity of pancreata, however, limits optical accessibility for high-resolution light microscopy of islets in situ. Because the standard microtome-base...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3470241
更新日期:2010-07-01 00:00:00
abstract::Nanophotothermolysis with long laser pulses for treatment of scattered cancer cells and their clusters is introduced with the main focus on real-time monitoring of temperature dynamics inside and around individual cancer cells labeled with carbon nanotubes. This technique utilizes advanced time- and spatially-resolved...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3119135
更新日期:2009-03-01 00:00:00
abstract::Optical microanglography (OMAG) is a recently developed imaging modality capable of volumetric imaging of dynamic blood perfusion, down to capillary level resolution, with an imaging depth up to 2.00 mm beneath the tissue surface. We report the use of OMAG to monitor the cerebral blood flow (CBF) over the cortex of mo...
journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.3207121
更新日期:2009-07-01 00:00:00
abstract::This work reviews our research during the past several years on time-resolved (TR) near-infrared diffuse optical tomography (DOT). Following an introduction of the measuring modes, two proposed schemes of image reconstruction in TR-DOT are described: one utilizes the full TR data, and the other, referred to as the mod...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.2815724
更新日期:2007-11-01 00:00:00