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Effects of caffeic acid phenethyl ester application on dentin MMP-2, stability of bond strength and failure mode of total-etch and self-etch adhesive systems.

Abstract：

OBJECTIVE:Investigate the long-term effect of dentin pretreatment with 0.05 or 0.1% caffeic acid phenethyl ester (CAPE) on (1) bond strength of resin composite to dentin by a three-step etch-and-rinse (Adper Scotchbond Multipurpose/ ASB) or a two-step self-etch adhesive system (Clearfil SE Bond/ CSE), (2) their fracture mode, (3) the micromorphological features of the hybrid layer formed; and (4) the level of MMP-2 in dentin (after application, using a correlative immunoexpression/quantification approach). DESIGN:Composite resin blocks were fabricated on 48 third molars (n = 6), according to the type of adhesive and treatment (control, CAPE 0.05% and CAPE 0.1%). Slices were obtained for scanning electron microscopy (SEM) evaluation, and sticks were fabricated for microtensile tests (24 h and 1 year). Aliquots of dentin powder were distributed (n = 12) according to the treatment and the MMP-2 concentration was determined by ELISA. RESULTS:Tukey test showed that ASB groups presented higher BS in 24 h than CSE groups. ASB presented a reduction in BS values after 1-year. ASB and CSE presented no significant differences in BS after 1-year. CAPE had no effect on BS for both adhesive systems. The predominant failure mode for the ASB groups were adhesive; when 0.1% CAPE was applied there was a predominance of mixed fractures. Regarding the CSE group, 0.05% CAPE led to more adhesive failures, and the 0.1% concentration resulted in a higher number of cohesive failures in dentin. Higher MMP-2 concentrations were detected for the groups that did not undergo demineralization treatment, and the lowest values for the ASB groups treated with CAPE. SEM analysis showed no influence of pretreatment with CAPE. CONCLUSIONS:CAPE did not influence the BS of the adhesives tested, or the micromorphology of the hybrid layer, irrespective of concentration or storage time. CAPE affected the fracture pattern at 24 h, depending on the concentration and the adhesive system used. Immunoassay analysis showed that CAPE 0.1% reduced the MMP-2 concentration in the ASB adhesive without affecting bond strength to dentin.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Pedrosa VO,França FMG,Turssi CP,Amaral FLBD,Teixeira LN,Martinez EF,Basting RT

doi

10.1016/j.archoralbio.2018.06.012

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

16-26

eissn

0003-9969

issn

1879-1506

pii

S0003-9969(18)30245-0

journal_volume

pub_type

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Effects of caffeic acid phenethyl ester application on dentin MMP-2, stability of bond strength and failure mode of total-etch and self-etch adhesive systems.