Abstract：
OBJECTIVE:To investigate the effects of periodontal bacterial lysates on maturation and function of mature monocyte-derived dendritic cells (m-MDDCs) derived from individuals with chronic periodontitis (CP) or healthy periodontal tissue (HP). DESIGN:m-MDDCs derived from peripheral blood monocytes, cultured for 7 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF), were stimulated with lysates of Streptococcus sanguinis, Prevotella intermedia, Porphyromonas gingivalis, or Treponema denticola on day 4, and were then phenotyped. IL-10, IL-12 and IFN-gamma concentration in the supernatant of cultures were measured. RESULTS:Expression of HLA-DR was lower in bacterial-unstimulated mature m-MDDC from CP compared to HP (p=0.04), while expression of CD1a and CD123 were higher in CP. The expression pattern of HLA-DR, CD11c, CD123, and CD1a did not change on bacterial stimulation, regardless of the bacteria. Stimulation with P. intermedia upregulated CD80 and CD86 in CP cells (p≤0.05). Production of IL-12p70 by bacterial-unstimulated m-MDDCs was 5.8-fold greater in CP compared to HP. Bacterial stimulation further increased IL-12p70 production while decreasing IL-10. Significantly more IFN-gamma was produced in co-cultures of CP m-MDDCs than with HP m-MDDCs when cells were stimulated with P. intermedia (p=0.009). CONCLUSIONS:Bacterial-unstimulated m-MDDC from CP exhibited a more immature phenotype but a cytokine profile biased towards proinflammatory response; this pattern was maintained/exacerbated after bacterial stimulation. P. intermedia upregulated co-stimulatory molecules and IFN-gamma expression in CP m-MDDC. These events might contribute to periodontitis pathogenesis.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Cury PR,Carmo JP,Horewicz VV,Santos JN,Barbuto JAdoi
10.1016/j.archoralbio.2013.03.013subject
Has Abstractpub_date
2013-09-01 00:00:00pages
1208-16issue
9eissn
0003-9969issn
1879-1506pii
S0003-9969(13)00107-6journal_volume
58pub_type
杂志文章abstract：:External apical root resorption (EARR) induced by orthodontic treatment and chronic periodontitis (CP) are complex phenotypes dependent on the interaction of multiple genetic and non-genetic risk factors. Apart from different environmental triggers, these phenotypes are caused by antagonistic biological mechanisms inv...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2018.08.006
更新日期：2018-11-01 00:00:00
abstract：:In the complex neurovascular control of the orofacial area, the only vasoconstrictor action is mediated by the sympathetic nervous system; however, its functional role is still unclear as little evidence exists of vasoconstrictor responses to physiological stimuli in both animal and human models. Aim of the present st...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.01.003
更新日期：2009-04-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Epithelial-mesenchymal transition (EMT) has been proved to occur in drug-induced gingival overgrowth. However, the specific pathogenic mechanism remains uncertain. The aim of this study is to examine the expression of EMT markers in cyclosporine A (CsA)-induced gingival overgrowth in rat models...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.024
更新日期：2017-09-01 00:00:00
abstract：:The aim of this study was to investigate the potential effects of vitamin K1 supplementation on skeletal changes related to fluoride in growing rats. Forty male Wistar rats aged 4 weeks were assigned at random into three groups: high-dose fluoride (125 ppm) group; high-dose fluoride + vitamin K1 (0.2 mg/(g day)) group...
journal_title：Archives of oral biology
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1016/j.archoralbio.2009.02.001
更新日期：2009-06-01 00:00:00
abstract：:Small GTPase Rab27 has been implicated in the regulation of different types of membrane trafficking, including melanosome transport and various regulated secretion events. We have previously shown that Rab27 and its effectors, Slac2-c/MyRIP and Slp4-a/granuphilin-a, are involved in the control of isoproterenol (IPR)-i...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.12.007
更新日期：2009-04-01 00:00:00
abstract：:Differences in the shapes of the Hunter-Schreger bands in different teeth and in different areas of a tooth have never been explained. They are almost certainly related directly to differences in the courses and organization of prisms. A computerized model was used to construct vertical sheets of enamel prisms whose c...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90065-i
更新日期：1990-01-01 00:00:00
abstract：:The surface zone of human deciduous and permanent enamel was examined following sectioning, polishing and etching. Three main types of surface were where prisms reached the surface, where the prisms were not discernible and those formed by a complex of both types. The aprismatic layers were measured and the percentage...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90147-9
更新日期：1982-01-01 00:00:00
abstract：:The dental pulp is richly innervated by peptidergic nociceptive neurones that are of special interest because of their central role in dental pain and because they have some features that are not typical of other somatic nociceptors. Here, (35)S-riboprobes were used to determine whether pulpal afferents of adult (2-mo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00002-9
更新日期：2000-05-01 00:00:00
abstract：OBJECTIVE:This study aimed to identify the optimal micro- and submicroscale topographies of titanium (Ti) substrata that would most significantly influence adhesion, proliferation, and other activities of these cells. DESIGN:Truncated V-shaped microgrooves in 60 μm-wide and 10 μm-deep cross-sections with 0°, 30°, 60°,...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.11.013
更新日期：2012-07-01 00:00:00
abstract：:Two experimental approaches were used to examine the relationship between c-fos gene expression and tissue proliferative responses. Beta-Adrenergic and muscarinic receptor stimulation yielded equivalent levels of c-fos expression, although only beta-adrenergic receptor agonists are reported as capable of eliciting DNA...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90088-5
更新日期：1989-01-01 00:00:00
abstract：OBJECTIVE:This study aimed to examine and compare the anti-caries effects of citrus lemon oil (CLO) and limonene in rats. METHODS:The minimal inhibitory concentrations of CLO and limonene were measured using the disk diffusion method. The rats were infected with Streptococcus sobrinus and assigned into four groups: (1...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104851
更新日期：2020-10-01 00:00:00
abstract：UNLABELLED:The inferior head of lateral pterygoid (IHLP) is thought to play a critical role in the generation and control of lateral jaw movements. AIM:The aim was to test the hypothesis that a change to the lateral tooth guidance (working-side occlusal alteration, OA) results in a significant change in the electromyo...
journal_title：Archives of oral biology
pub_type： 临床试验,杂志文章
doi：10.1016/j.archoralbio.2006.01.007
更新日期：2006-08-01 00:00:00
abstract：OBJECTIVE:Secondary cartilage develops from osteochondral progenitor cells. Hypertrophic chondrocytes in secondary cartilage increase within a very short time and then ossify rapidly. In the present study, we investigated the sequential development process of osteochondral progenitor cells, and the morphology and size ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.12.027
更新日期：2018-05-01 00:00:00
abstract：:Masseter and digastric muscle activities and jaw movement trajectories were recorded in freely moving rabbits during eating. The patterns in these trajectories and activities were similar to those described in previous studies on restrained animals. Although the duration of masticatory sequences, which started with fo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90099-t
更新日期：1992-01-01 00:00:00
abstract：:A laminin receptor was isolated from bovine gingival epithelial-cell membrane. After solubilization with octylglucoside, the receptor was subjected to affinity chromatography on laminin-coupled Sepharose and eluted with cation-free EDTA buffer yielding on SDS-PAGE a 67 kDa protein band. After radioiodination, the prot...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90003-D
更新日期：1991-01-01 00:00:00
abstract：:Cell shape in salivary glands is affected by mechanical forces. In the acini and ducts cell shape is modified by the contractions of the myoepithelial cells in both the secretory and ductal portions of the glands. At the organ level shape changes are due to muscle contraction during mastication, food intake and speech...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：OBJECTIVE:The radical anion of oxygen (O(-)) is extremely oxidative and shows high reactivity. In this study, the antibacterial activity of water super-oxidised water containing high concentration of O(-) (O(-)-water) was tested against cultured planktonic cells of cariogenic bacteria, periodontopathic bacteria and Can...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.03.002
更新日期：2010-06-01 00:00:00
abstract：:Simultaneous recordings of the activity of the masseter and anterior temporalis and of jaw movement were made on 10 healthy dentate volunteers while they chewed roasted peanuts in their habitual manner. The quantity of food taken was altered by varying (a) its total weight (mouthful) between 8 and 1 g, but with a fixe...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90120-j
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:This case-control study aimed to investigate associations between craniofacial pain complaints, somatoform symptoms and chronic diseases. DESIGN:306 subjects were included in this study (174 patients and 132 healthy subjects). The evaluation consisted of demographic data, number of functional and nonfunction...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104892
更新日期：2021-02-01 00:00:00
abstract：:Stimulation of muscarinic cholinergic receptors on rat parotid acinar cells causes a rapid production of inositol phosphates, with the key metabolic event being the breakdown of phosphatidylinositol 4,5-bisphosphate into inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and diacylglycerol. Here a high-performance liquid chr...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00109-6
更新日期：2001-04-01 00:00:00
abstract：OBJECTIVES:Dental caries is the most common chronic infectious disease in children. Streptococcus mutans, the main cariogenic bacterial species, produces persisters, nongrowing dormant variants of regular cells associated with chronicity of diseases. We hypothesized that the recurrent nature of caries, particularly wit...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.104601
更新日期：2020-02-01 00:00:00
abstract：:Previous studies using potassium ion-selective microelectrodes have demonstrated that potassium concentrations in dental plaque fluid obtained by centrifugation are identical to whole plaque values determined immediately after collection. Such procedures were now used to examine the variations in potassium concentrati...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90030-1
更新日期：1988-01-01 00:00:00
abstract：:A phenotypic study had shown that gingival B cells respond differently to two periodontopathic bacteria, Porphyromonas gingivalis and Fusobacterium nucleatum. Further investigation now shows a reduction in the percentage of Ki-67 + T cells in cultures of gingival and peripheral blood mononuclear cells stimulated with ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90139-y
更新日期：1992-01-01 00:00:00
abstract：:Although it is well known that somatostatin (SRIF) modulates several digestive functions, there are only a few reports about its effect on the salivary glands. Here, the action of SRIF parotid secretion was studied, in vivo and in vitro, in male Wistar rats. In vivo SRIF infusion (35 microg/kg per hr) inhibited the pa...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00035-7
更新日期：1999-07-01 00:00:00
abstract：:Recent reports characterizing the physiological properties of normal human labial acini are reviewed with particular emphasis on mechanisms related to fluid secretion. In contrast to the salivary glands of several experimental animals, human labial acinar cells do not appear to have a1-adrenergic receptors, substance ...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/s0003-9969(99)00044-8
更新日期：1999-05-01 00:00:00
abstract：:Proteases produced by Porphyromonas gingivalis are believed to contribute to the pathogenesis of periodontal diseases. Here the cytotoxic effects of a purified preparation of a P. gingivalis protease with trypsin-like specificity were tested on human gingival fibroblasts in vitro. The active protease induced apoptotic...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00002-3
更新日期：1999-04-01 00:00:00
abstract：:Previous studies have demonstrated that low-magnitude, high-frequency (LMHF) mechanical vibrations (LM<1g; HF<20-90Hz) can benefit bone formation. However, the underlying mechanism is unclear. In this study, MC3T3-E1 cells were exposed to LMHF vibrations of different magnitudes to assess the effect of vibrations on β-...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.06.009
更新日期：2016-10-01 00:00:00
abstract：UNLABELLED:Tooth enamel is formed by ameloblasts, which are derived from the epithelial cells of the enamel organ. OBJECTIVE:The purpose of this study was to grow human ameloblast-like epithelial cells in culture. DESIGN:Human fetal tooth organs were isolated, and the cells were separated by digestion in collagenase/...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.12.008
更新日期：2005-08-01 00:00:00
abstract：:The effects of dietary calcium deficiency and subsequent replenishment of the diet with calcium alone or with xylitol were studied. Thirty 3-week-old Wistar rats were labelled with an i.p. tetracycline injection. Twenty rats were fed a diet with 0.026% calcium (Ca-deficient); 10 received a 0.5% Ca diet (controls). Aft...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00036-o
更新日期：1995-08-01 00:00:00
abstract：:There was a statistically significant shift towards increased proportions of type I Langerhans cells (containing many Langerhans-cell granules) and reduced proportions of both type II Langerhans cells (containing few granules) and indeterminate cells in diseased oral epithelium when compared to healthy oral epithelium...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90004-x
更新日期：1986-01-01 00:00:00
