Abstract:
BACKGROUND AND OBJECTIVE:Epithelial-mesenchymal transition (EMT) has been proved to occur in drug-induced gingival overgrowth. However, the specific pathogenic mechanism remains uncertain. The aim of this study is to examine the expression of EMT markers in cyclosporine A (CsA)-induced gingival overgrowth in rat models. MATERIAL AND METHODS:Thirty-six rats were randomly divided into two groups. The experimental group received CsA therapy subcutaneously in a daily dose of 10mg/kg, and the other group was used as a control. Six rats per group were sacrificed at 20, 40 and 60days, and the gingivae were obtained. The expression of TGF-β1, E-Cadherin, ZEB1, ZEB2, and Snail1 were examined by quantitative real time PCR (qRT-PCR), western blotting, and immunohistochemistry. In addition, a group of microRNAs associated with EMT and fibrosis were also detected in gingival tissue by qRT-PCR. RESULTS:The mRNA and protein levels of TGF-β1, ZEB1, and ZEB2 in gingivae were significantly upregulated after 40 and 60days of CsA administration. Conversely, the levels of E-cadherin were significantly downregulated in overgrowth sample at day 40 and 60. Intense immunohistochemmical staining for TGF-β1 were observed in the samples from CsA group at day 40 and 60. Concomitantly, the densities of E-cadherin were gradually decreased in the basal layers of epithelium with time. Three members of miR-200s (miR-200a, miR-200b and miR-200c) were significantly downregulated in CsA-treated rats at 40 and 60days, while miR-9, miR-23a and miR-155 were significantly upregulated when compared with those of the control group. CONCLUSIONS:The process of EMT in CsA-induced rat gingival overgrowth is associated with increased expression of TGF-β1, ZEB1, and ZEB2, and decreased expression of E-cadherin.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Yang F,Lu J,Yu Y,Gong Ydoi
10.1016/j.archoralbio.2017.04.024subject
Has Abstractpub_date
2017-09-01 00:00:00pages
48-55eissn
0003-9969issn
1879-1506pii
S0003-9969(17)30134-6journal_volume
81pub_type
杂志文章abstract::Two experimental approaches were used to examine the relationship between c-fos gene expression and tissue proliferative responses. Beta-Adrenergic and muscarinic receptor stimulation yielded equivalent levels of c-fos expression, although only beta-adrenergic receptor agonists are reported as capable of eliciting DNA...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90088-5
更新日期:1989-01-01 00:00:00
abstract::Intrusive displacement of 7 incisors was measured before and within 3 min of extraction. A substantial increase in intrusion with recovery from loads of 4 N followed replacement of the incisors. These changes are taken to indicate that tension is an important mechanism of support in the intact periodontal ligament. ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90057-2
更新日期:1991-01-01 00:00:00
abstract::A genomic library of Bacteroides gingivalis W83 chromosomal DNA was constructed in the Escherichia coli lambda (lambda) vector EMBL 4. Three recombinant lambda phages expressing a cloned protease were identified in the library. All three lambda phages contained cloned overlapping DNA fragments from the same region of ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(90)90137-y
更新日期:1990-01-01 00:00:00
abstract::Parotid salivation was stimulated by infusion of bethanechol chloride into anaesthetized brushtail possums to ascertain maximal flow rates, salivary composition and dietary adaptations of salivary function. Secretion rates for one gland ranged from 5.3 +/- 0.16 to 84.3 +/- 3.20 microliters/min (2.4 +/- 0.07 to 37.8 +/...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90127-9
更新日期:1994-04-01 00:00:00
abstract::Cementum of teeth previously analysed for fluoride was re-examined in order to determine whether or not periodontal disease had affected the thickness of the tissue. In the periodontally diseased teeth the cementum was thinner than normal in the middle region of the root. The apical cementum, however, was significantl...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90132-r
更新日期:1992-08-01 00:00:00
abstract:OBJECTIVES:Reconstruct childhood diet using teeth collected in Edmonton in a collaborative effort between the Departments of Dentistry and Anthropology at the University of Alberta. METHODS:Deciduous teeth needing extraction were collected from 33 children for stable isotope ratio analysis of diet. Tooth dentin was mi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.07.014
更新日期:2014-11-01 00:00:00
abstract::Adhesion molecules are considered to have an active role in controlling cell differentiation, although the mechanisms involved have yet to be determined. The developing tooth provides an excellent model to use for determining the factors/processes regulating cell differentiation. The studies presented here focused spe...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(96)00051-9
更新日期:1996-08-01 00:00:00
abstract::The effect of muscle temperature on the speed of contraction and on the strength of contraction of the cat jaw muscles was studied. Muscles contracted faster when their temperatures were increased. The tetanic and twitch tension decreased significantly as the animal was heated from 30 to 40 degrees C. ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90030-x
更新日期:1984-01-01 00:00:00
abstract::Previous studies of the sympathetic nerve supply of the rat mandibular incisor pulp have shown conflicting results. Here, the neurovascular control of the rat lower incisor pulp was investigated by stimulating the tooth crown and the cervical sympathetic trunk electrically and monitoring blood-flow changes in the pulp...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90033-5
更新日期:1992-12-01 00:00:00
abstract:OBJECTIVE:Recent studies have showed that genetic factors involved in the host responses might determine the severity of periodontitis. hCAP-18/LL-37 is a part of the innate immune response in the oral cavity. The aim of the present study was to investigate the mutation of CAMP gene encoding hCAP-18/LL-37 in the patien...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.11.016
更新日期:2011-06-01 00:00:00
abstract::The response to stimulation of the parasympathetic innervation to parotid or submandibular glands of reserpinized rats was altered from that of untreated rats. Thus, acute reserpinization, like other types of sympathectomy, resulted in increase in volume of parasympathetically-evoked parotid or submandibular saliva wh...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(84)90040-2
更新日期:1984-01-01 00:00:00
abstract::For the rate of demineralization of enamel during caries, three physicochemical processes are of importance: the diffusion into and out of the enamel, the dissolution of mineral and the complexation of ions. The interpretation of diffusion experiments in conjunction with artificial caries experiments and computer simu...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/0003-9969(83)90006-7
更新日期:1983-01-01 00:00:00
abstract::The forces that develop in the human temporomandibular joint during function have never been directly measured, yet many patients exhibit excessive localized wear and tear of the joint, suggesting that at times these forces exceed tissue tolerance. The purpose here was to gain insight into vibration transmission betwe...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(01)00068-1
更新日期:2001-11-01 00:00:00
abstract::The possible role of fibronectin in dental tissue repair was investigated by comparing its distribution and that of the 165-kDa fibronectin-binding membrane protein (165 kDa-FnBP) in odontoblasts underlying carious and sound dentine. By immunoperoxidase and light microscopy, fibronectin was localized in the dentine un...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00068-z
更新日期:1995-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the expression of matrix metalloproteinase inducer (EMMPRIN) and its correlation with the expression of matrix metalloproteinases (MMPs)-1, -2 and -9 during the development of periapical lesion in mice. METHODS:Periapical lesions were induced in the lower first molars of mice and after 7, 14, 21 ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.05.021
更新日期:2014-09-01 00:00:00
abstract::Designing a temporomandibular joint (TMJ) total prosthesis requires the assessment of joint displacements for open/close movements. Current knowledge presents disc-condyle motions as involving both translation and rotation but there may be substantial variations from human to human. The aim of this study is to discrim...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.11.010
更新日期:2008-05-01 00:00:00
abstract::A variable capacitance displacement transducer, previously employed for continuously monitoring movements of the rabbit mandibular incisor, was modified for use with the ferret mandibular canine. Comparing this tooth of limited growth with the tooth of continuous growth, similarities were seen in the reactions to extr...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90177-2
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:This study investigated the effect of subinhibitory concentrations of gentian violet on the germ tube formation by Candida albicans and its adherence ability to oral epithelial cells. METHODS:Thirty strains of C. albicans isolated from denture wearers, normal healthy individuals and HIV positive patients wer...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.05.016
更新日期:2017-10-01 00:00:00
abstract::Both Sjögren's syndrome and therapeutic irradiation for head and neck cancer lead to irreversible damage of the parenchyma of the salivary glands. This report describes an attempt to grow miniature pig (minipig) parotid gland cells on artificial films and tubular scaffolds with the ultimate intention of developing bio...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2005.10.001
更新日期:2006-05-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine whether measurements of volatile sulfur compounds (VSCs) are useful to predict colonization of periodontopathic bacteria. For this purpose, we assessed the relationships among distributions of 4 species of periodontopathic bacteria in tongue coating and dental plaque, o...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.09.019
更新日期:2013-03-01 00:00:00
abstract::An immunofluorescence study of the expression of keratin 14 (K14) during the formation of filiform papillae was performed and the progress of keratinization of the epithelium of the rat tongue was monitored on semi-ultrathin sections by laser-scanning microscopy. Differential interference contrast (DIC) images were al...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(03)00118-3
更新日期:2003-08-01 00:00:00
abstract::Mandibular first-molar tooth germs were dissected from 16-day-embryo and new-born CD1 mice. By incubation in collagenase they were separated into enamel organ, papilla and follicle. Dental pulp was obtained from mandibular first molars of 3-, 7- and 10-day-old mice. Various combinations of epithelial and mesenchymal t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90022-7
更新日期:1987-01-01 00:00:00
abstract::Sixteen consecutively-born monkeys were used in a longitudinal study to determine the changes occurring in the total number of bacteria in the palatal grooves of the upper left first deciduous molar teeth, following the introduction of a sucrose-based diet. The total number of bacteria recovered from the grooves incre...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(85)90029-9
更新日期:1985-01-01 00:00:00
abstract::The dental pulp is richly innervated by peptidergic nociceptive neurones that are of special interest because of their central role in dental pain and because they have some features that are not typical of other somatic nociceptors. Here, (35)S-riboprobes were used to determine whether pulpal afferents of adult (2-mo...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00002-9
更新日期:2000-05-01 00:00:00
abstract::Many studies have shown that various myosin isoforms are involved in muscle contraction. A search for specific antibodies directed against the myosin heavy chain (MHC) resulted in the identification of at least two main classes, referred to as MHC type I and type II. In this study, immunohistology and gel electrophore...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00092-3
更新日期:2000-12-01 00:00:00
abstract:OBJECTIVE:To investigate the effects of sitagliptin, a dipeptidyl peptidase 4 inhibitor used to treat type II diabetes, on bone tissue and on implant osseointegration in diabetic rats. DESIGN:Thirty-two male rats were divided into four groups: 1) Diabetic animals (GD); 2) Diabetic animals that received sitagliptin (GD...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.04.018
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:Epithelial cells participate in wound healing by covering wounds, but also as important mediators of wound healing processes. Topical application of the phospholipid growth factor lysophosphatidic acid (LPA) accelerates dermal wound healing and we hypothesized that LPA can play a role in human oral wound heal...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.04.008
更新日期:2017-08-01 00:00:00
abstract::There may be deleterious effects if there is an age-dependent reduction in mucin composition or content in salivary glands. To assess whether there was an effect of age in submandibular glands from male mice aged 3 months, 10 and 20 months and 27, 29 and 30 months, mucin, protein and sialic acid were quantitated. Chan...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90139-l
更新日期:1991-01-01 00:00:00
abstract::Serotonin (5-HT) stimulates tooth-germ development in embryonic mouse mandibular explant cultures, but it is not clear whether this is due to a direct action on epithelial-mesenchymal interactions, or whether development was stimulated indirectly by serotonergic regulation of other morphoregulatory molecules. A calciu...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(98)00067-3
更新日期:1998-10-01 00:00:00
abstract::Mechanisms of primary fluid formation by macropodine mandibular glands were investigated in anaesthetized red kangaroos using ion-transport and carbonic anhydrase inhibitors. Bumetanide at carotid plasma concentrations of 0.005-0.1 mmol/l progressively reduced a stable, acetylcholine-evoked flow rate of 1.02 +/- 0.024...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(97)00074-5
更新日期:1997-10-01 00:00:00