Abstract：
UNLABELLED:Tooth enamel is formed by ameloblasts, which are derived from the epithelial cells of the enamel organ. OBJECTIVE:The purpose of this study was to grow human ameloblast-like epithelial cells in culture. DESIGN:Human fetal tooth organs were isolated, and the cells were separated by digestion in collagenase/dispase. The cells were cultured in KGM-2 media with and without serum and at different calcium concentrations. The expression of enamel matrix proteins was analyzed by RT-PCR and cytokeratin 14 was detected by immunohistochemistry. The cells were further characterized by osteogenesis/odontogenesis-related DNA array. RESULTS:Cells isolated from the tooth organs grown in KGM-2 media containing 2-10% serum, were mixture of cobblestone and spindle shaped cells. Culturing these cells in KGM-2 with 0.05 mM calcium was selective for cobblestone ameloblasts-like cells (CAB), which were immunopositive for cytokeratin 14. Amelogenin, ameloblastin, enamelin, MMP-20 and KLK-4 were detected in CAB cells by RT-PCR. Osteogenesis SuperArray analyses could not detect the presence of typical molecules related to mesenchymal odontoblast or osteoblast lineage cells in these cultures. CONCLUSIONS:These studies showed that cobblestone-shaped ameloblast-like cells are selected from the tooth organ cells, by culture in KGM-2 media with 0.05 mM calcium.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
DenBesten PK,Machule D,Zhang Y,Yan Q,Li Wdoi
10.1016/j.archoralbio.2004.12.008subject
Has Abstractpub_date
2005-08-01 00:00:00pages
689-94issue
8eissn
0003-9969issn
1879-1506pii
S0003-9969(05)00009-9journal_volume
50pub_type
杂志文章abstract：:Non-specific cholinesterase (ChE) activity was demonstrated in lamellated sensory corpuscles of the rat lip by light and electron microscopy using Karnovsky and Roots' method. ChE activity was present in the interlamellar spaces between neighbouring lamellae as well as in the peri-axonal space between axon terminals a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90056-2
更新日期：1987-01-01 00:00:00
abstract：OBJECTIVE:Obtaining high number of stem cells is of interest for cell based therapies. N-Acetyl-l-cysteine (NAC) acts as a source of sulfhydryl groups and an anti-oxidative agent. The aim of this study was to test different NAC concentration on proliferation and differentiation of deciduous teeth dental pulp stem cells...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.06.002
更新日期：2016-10-01 00:00:00
abstract：:Destrin, an actin-binding protein, is partly phosphorylated at Ser-2 (numbering on the matured form) in the resting rat parotid gland, and beta-adrenergic or cholinergic stimulation of this gland induces its dephosphorylation. In this study, we searched for the protein kinase involved in phosphorylation of destrin. We...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00129-8
更新日期：2003-09-01 00:00:00
abstract：OBJECTIVE:Replacement teeth in reptiles and mammals develop from a successional dental lamina. In monophyodont (single generation) species such as the mouse, no successional lamina develops. We have selected a reptilian monophyodont species - the Veiled Chameleon (Chamaeleo calyptratus) - to investigate whether this is...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.10.019
更新日期：2013-02-01 00:00:00
abstract：:One hundred and forty-two teeth of children and adults were analysed for lead content by graphite furnace atomic absorption spectrophotometry. The results showed that, for children, tooth age is an important factor that has to be taken into consideration when lead levels are evaluated. In adults tooth type may be igno...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(90)90069-m
更新日期：1990-01-01 00:00:00
abstract：OBJECTIVE:Genistein is a major isoflavone subclass of flavonoids found in soybean and a potent tyrosine kinase inhibitor. The present study aimed to assess the effect of genistein on the production of proinflammatory mediators in murine macrophages stimulated with lipopolysaccharide (LPS) isolated from Prevotella inter...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.11.019
更新日期：2016-02-01 00:00:00
abstract：:Women with reproductive capability are more likely to suffer from temporomandibular disorders (TMD), with orofacial pain as the most common complaint. In the past, we focused on the role of estradiol in TMD pain through the nervous system. In this study, we explored estradiol's influence on synoviocyte gene expression...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2015.05.011
更新日期：2015-09-01 00:00:00
abstract：:Sample groups of children (n=50) and adults (n=38) were selected from pools of 207 children, (11-13-year olds from two primary schools) and 94 adults (25-44-year olds from four governmental agencies) who were the subjects of an oral health survey among Tibetans living in Lhasa, Tibet Autonomous Region. Mean ages of th...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(02)00201-7
更新日期：2003-02-01 00:00:00
abstract：:The application of detergent solutions to dissociated molar dental papillae of mice permitted precise observation of the extracellular fibrillar material in the odontoblast layer and the predentine. Two types of fibrils were identified: cross-banded fibrils of variable diameter (25-200 nm) and aperiodic microfibrils o...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90092-1
更新日期：1988-01-01 00:00:00
abstract：:The aim of this project was to develop an analytical procedure with the required level of sensitivity for the determination of glucose concentrations in small volumes of unstimulated fasting whole saliva. The technique involves high-performance ion-exchange chromatography at high pH and pulsed amperometric detection. ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00121-2
更新日期：1996-02-01 00:00:00
abstract：AIMS:Anomalies of tooth number may not be isolated conditions but may have wider associations in the development of the dentition including tooth size. This study aimed to examine links between hypodontia, supernumerary teeth and crown size, considering the effect on the development of the whole dentition and so increa...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.05.016
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVE:To investigate the antimicrobial effect of alpha-bisabolol and tea tree oil alone and in combination against the halitosis-associated Gram-positive bacillus Solobacterium moorei. DESIGN:The inhibitory activity of alpha-bisabolol and tea tree oil against the reference strain S. moorei CCUG39336 and four clini...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.08.001
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:Orofacial clefts result from failures of developing embryonic facial and palatal processes to either completely merge or fuse. Normal development of the facial primordia requires remodelling of the extracellular matrix, which is mediated in part by the matrix metalloproteinases (MMPs). MMPs can be considered ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.04.005
更新日期：2007-10-01 00:00:00
abstract：:Internal derangement (ID) in the temporomandibular joint (TMJ) comprises a group of clinical problems with relatively high prevalence. However, the temporal changes in gene expression of condylar cartilage during continuous ID remain unclear. The aim of the current study is to investigate by microarray analysis, the d...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.11.006
更新日期：2017-02-01 00:00:00
abstract：OBJECTIVES:The aim of the present study was to identify the anticancer effects and the mechanisms of action of shikonin and its analogue isobutyrylshikonin in oral squamous carcinoma cells. DESIGNS:The cytotoxic effects of isobutyrylshikonin and shikonin in Ca9-22 and SCC-25 cells were analyzed using 3-(4,5-dimethylth...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104774
更新日期：2020-08-01 00:00:00
abstract：OBJECTIVE:The complex biological, physicochemical process of human dental enamel formation begins in utero and for most teeth takes several years to complete. Lost enamel tissue cannot regenerate, therefore a better understanding of the spatial and temporal progression of mineralization of this tissue is needed in orde...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.08.012
更新日期：2013-11-01 00:00:00
abstract：:To investigate whether mechanical deformation of osteoblasts exerts an influence upon parathyroid hormone (PTH)-induced bone resorption, the effect of medium conditioned by a statistically stretched, osteoblast-enriched, bone cell population on PTH-induced osteoclast-like cell formation in mouse bone marrow cultures w...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/S0003-9969(97)00001-0
更新日期：1997-03-01 00:00:00
abstract：:The study examined the glucose clearance (retention) in saliva at different surfaces of these teeth in 23 subjects. The mouth was thoroughly rinsed for 15 s with 20 ml of a 0.5 M glucose solution. The concentrations of glucose absorbed by small pieces of paper, placed on the mesial, distal, labial (buccal), lingual an...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90183-m
更新日期：1993-06-01 00:00:00
abstract：OBJECTIVE:The DNA oxidation byproduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a well-known biomarker used to evaluate oxidative stress. We previously reported that the generation of reactive oxygen species (ROS) is increased in cultured gingival fibroblasts (GF) from patients with Down syndrome (DS). Thus, the aim of t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.03.017
更新日期：2013-09-01 00:00:00
abstract：:The present study was designed to investigate the mechanical properties and load-relaxation of these discs in compression. Eight discs were used for the experiments. Compression was applied to specimens up to the specified strain, and a series of load-relaxation tests was conducted on each specimen from 0.25% strain t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00097-7
更新日期：1999-12-01 00:00:00
abstract：OBJECTIVE:To study the potential chemotherapeutic effects of Diosgenin, zoledronic acid and Epigallocatechin-3-gallate on oral squamous cell cancer (OSCC). MATERIALS AND METHODS:Cell viability, migration, apoptosis and cell cycle evaluation assays were performed in order to assess the effects of different doses of Dio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.05.023
更新日期：2017-10-01 00:00:00
abstract：:The effects of ligation on body weight, protease activity and hair kynurenine content were observed. The body weights of parotid gland(PT)-ligated and of combined submandibular (SM)-, sublingual (SL)- and PT-ligated rats were much less than those of sham-operated controls. However, the weights of SM,SL-ligated rats we...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90137-9
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVES:The objective of this study was to evaluate the effect of platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF) on bone healing. STUDY DESIGN:Twelve rabbits were included in this randomized, blinded, prospective study. 15-mm×10-mm-sized defects were created in the pari...
journal_title：Archives of oral biology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.archoralbio.2014.02.004
更新日期：2014-05-01 00:00:00
abstract：:The purpose of the study was to test the hypothesis that both insoluble pure type I collagen from bovine Achilles tendon and dentine collagen in root dentine powder from human teeth required acid pretreatment for subsequent degradation by trypsin, a non-specific protease. Pure type I collagen or dentine powder was tre...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(94)90022-1
更新日期：1994-10-01 00:00:00
abstract：:The expression of anchorage independence in malignant oral epithelial cells retrieved from colonies formed in agarose and tumours formed in athymic mice was examined. The original epithelial cell lines were derived from lingual and palatal squamous cell carcinomas induced in rats by the carcinogen 4-nitroquinoline N-o...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90143-x
更新日期：1989-01-01 00:00:00
abstract：:The soluble N-ethylmaleimide-sensitive fusion (NSF) attachment protein (SNAP) receptor (SNARE) hypothesis has been applied to exocytosis in salivary glands. The expression of SNARE proteins has not been well investigated in the parotid gland. In this study, the mRNA expression of SNAREs and membrane-fusion-related pro...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00048-6
更新日期：2001-10-01 00:00:00
abstract：:In the hard palate, sagittally oriented capillary loops, 8-10 microns in diameter and 70-250 microns high, extended perpendicularly from a subcapillary plexus to the connective tissue papillae. These loops formed a well-delineated vascular spine beneath the rugal crests. Capillary loop density was estimated to be 200-...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90088-c
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:Streptococcus mutans, known to be a major pathogen of dental caries, is also considered to cause infective endocarditis. Its 120-kDa Cnm protein binds to type I collagen, which may be a potential virulence factor. In this study, we characterized S. mutans clinical strains focusing on the cnm gene encoding Cnm...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2009.11.008
更新日期：2010-01-01 00:00:00
abstract：:The biological activity of carboxyl-terminal parathyroid hormone peptide[53-84] (PTH[53-84]) and aminoterminal peptide [1-34] (PTH[1-34]) during cultivation of the lower molar tooth germ from mouse embryos was examined. PTH[1-34] increased alkaline phosphatase activity in the tooth germ at an early developmental stage...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(97)00107-6
更新日期：1998-04-01 00:00:00
abstract：:Growth in body length, skeletal lengths, incisor lengths and molar root lengths were retarded in dwarf mice. The root lengths of third molars were more severely affected than those of other molars. The crown lengths at the enamel-cementum junction of upper and lower molars of most dwarf mice were significantly shorter...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90135-9
更新日期：1985-01-01 00:00:00
