Abstract:
OBJECTIVES:Dental caries is the most common chronic infectious disease in children. Streptococcus mutans, the main cariogenic bacterial species, produces persisters, nongrowing dormant variants of regular cells associated with chronicity of diseases. We hypothesized that the recurrent nature of caries, particularly within populations with high-caries risk, is due partly to specific phenotypic features of S. mutans such as its ability to form persisters. We aimed to investigate the genotypic and phenotypic differences between the S. mutans from children with severe early-childhood caries (S-ECC) and those without caries. METHODS:S. mutans from plaque samples of caries-free (CF) and S-ECC children were tested for their ability to adapt to a lethal pH in an acid tolerance response assay. The persister levels of S. mutans isolates was quantified in both groups. RESULTS:S. mutanswas identified in all 23 S-ECC but only 6 of the 21 CF subjects. In most subjects, only one dominant S. mutans genotype was detected. No statistically significant differences in the mean survival percentage of S. mutans were observed between the two groups at a lethal pH of 3.5. However, the dominant genotype within a particular S-ECC subject exhibited a higher percentage of cell survival compared to those in the CF group. In S-ECC patients, S. mutans isolates displayed a ∼15-fold higher persistence phenotype than S. mutans isolates from CF patients. CONCLUSIONS:The ability of S. mutans to produce high levels of persisters may contribute to part of an individual's ability to control caries disease activity and recurrent lesions.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Bottner A,He RY,Sarbu A,Nainar SMH,Dufour D,Gong SG,Lévesque CMdoi
10.1016/j.archoralbio.2019.104601subject
Has Abstractpub_date
2020-02-01 00:00:00pages
104601eissn
0003-9969issn
1879-1506pii
S0003-9969(19)30847-7journal_volume
110pub_type
杂志文章abstract::Small amalgam fillings were placed in maxillary and mandibular second molar and canine teeth for cine-radiographic analysis. The rosette strain gauges were bonded bilaterally to mandibular cortical bone below the second or third molars. The monkeys were placed in a restraining chair that did not restrict normal head, ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90139-1
更新日期:1986-01-01 00:00:00
abstract::Garlic (Allium sativum) has long been known to have antibacterial, antifungal and antiviral properties but there are few data on its effects against oral bacterial species particularly putative periodontal pathogens or their enzymes. Filter sterilised, aqueous extract of garlic was tested for ability to inhibit the gr...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2004.12.002
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVE:To investigate interactions between hyaluronic acid (HA), lysozyme, and the glucose oxidase-mediated lactoperoxidase (GO-LPO) system in enzymatic and candidacidal activities. DESIGN:The influences of HA (0.5, 1.0, and 2.0mg/mL) and lysozyme (30μg/mL hen egg white lysozyme) on the enzymatic activity of GO-LPO...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2013.06.015
更新日期:2013-10-01 00:00:00
abstract::Osteoclastogenesis is the major cellular event responsible for bone loss and is triggered by inflammation. Acai-berry has proven anti-inflammatory effects. However, there is a lack of evidence for its effects on osteoclastogenesis. Thus, the aim of this study was to determine whether acai-berry extract (ABE) could inh...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.03.016
更新日期:2016-08-01 00:00:00
abstract::The origin of ureolytic activity in artificial-mouth plaques was established by assessing the contribution to plaque ureolytic activity of the isolated bacteria. To overcome losses of ureolytic activity caused by the unstable presence of urease in oral bacteria, ureolytic bacteria were isolated from an exceptionally a...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(88)90006-4
更新日期:1988-01-01 00:00:00
abstract::Recent reports characterizing the physiological properties of normal human labial acini are reviewed with particular emphasis on mechanisms related to fluid secretion. In contrast to the salivary glands of several experimental animals, human labial acinar cells do not appear to have a1-adrenergic receptors, substance ...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/s0003-9969(99)00044-8
更新日期:1999-05-01 00:00:00
abstract::The aim of this project was to develop an analytical procedure with the required level of sensitivity for the determination of glucose concentrations in small volumes of unstimulated fasting whole saliva. The technique involves high-performance ion-exchange chromatography at high pH and pulsed amperometric detection. ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00121-2
更新日期:1996-02-01 00:00:00
abstract::Smears of buccal mucosa, dorsal surface of the tongue and floor of mouth were taken from 10 patients with histologically confirmed oral lichen planus and 12 healthy age- and sex-matched controls. In buccal smears, no significant differences in cytoplasmic and nuclear areas were observed between lesional, adjacent non-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00106-9
更新日期:1996-01-01 00:00:00
abstract::Masticatory efficiency depends upon the ability of the molar cusps to apply concentrated bite forces to food particles and simultaneously to withstand the dental stresses that may cause enamel fracture. This study investigated how low-crowned molar cusps in omnivorous mammals, specifically humans, Homo sapiens, and pi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(00)00102-3
更新日期:2001-01-01 00:00:00
abstract::This study was undertaken to reveal detailed changes in fluoride distribution at different developmental stages of upper incisor enamel under various fluoride administration regimes. Four groups of Wistar rats received water containing 0, 25, 50 and 100 parts/10(6) fluoride respectively for 10 weeks. Five different en...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(88)90119-7
更新日期:1988-01-01 00:00:00
abstract::The change in expression of MUC1 from health to disease forms the basis of its use as a potential disease marker. Previous attempts at isolating MUC1 from normal, healthy human oral mucosa have, however, drawn conflicting conclusions as to its presence. Furthermore, when MUC1 was detected in the oral glycocalyx, it wa...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(01)00010-3
更新日期:2001-06-01 00:00:00
abstract::There is evidence to suggest that some reimplanted and autotransplanted teeth are reinnervated, but the time-course of reinnervation and the properties and source of the reinnervating axons are not known. This reinnervation has been investigated using electrophysiological techniques in 6 adult cats. In each cat, one l...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90172-3
更新日期:1983-01-01 00:00:00
abstract::The purpose of this study is to investigate the radioprotective effect of vitamin E as a natural product. Vitamin E protects the salivary glands dysfunction that is induced by ionizing radiation. It was analysed with radioisotope scintigraphy and then salivary gland to background counts ratio was calculated. Histopath...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.06.003
更新日期:2015-09-01 00:00:00
abstract::Evidence was sought for urea-induced pH gradients in dental plaque microcosm biofilms cultured from the mixed salivary bacteria in a multi plaque 'artificial mouth'. Application of 500 mmol/l urea for short periods (6 min) to 5-8 mm maximum-thickness plaques induced intraplaque pH gradients of up to 0.7 pH units with ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)90147-3
更新日期:1994-06-01 00:00:00
abstract::Adhesion molecules are considered to have an active role in controlling cell differentiation, although the mechanisms involved have yet to be determined. The developing tooth provides an excellent model to use for determining the factors/processes regulating cell differentiation. The studies presented here focused spe...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(96)00051-9
更新日期:1996-08-01 00:00:00
abstract::Previous studies have demonstrated that low-magnitude, high-frequency (LMHF) mechanical vibrations (LM<1g; HF<20-90Hz) can benefit bone formation. However, the underlying mechanism is unclear. In this study, MC3T3-E1 cells were exposed to LMHF vibrations of different magnitudes to assess the effect of vibrations on β-...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.06.009
更新日期:2016-10-01 00:00:00
abstract:OBJECTIVE:Non-syndromic supernumerary teeth (NSST) are a common type of dental anomaly. microRNAs (miRNAs) play an important role in craniofacial and tooth development. Therefore, we hypothesized that single nucleotide polymorphisms (SNPs) of miRNAs may be associated with the susceptibility of NSST. MATERIALS AND METH...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2020.104771
更新日期:2020-09-01 00:00:00
abstract::The aim of this study was to investigate the age-related developmental changes of taste bud distribution within the subpopulations at different postnatal ages in the mouse oral cavity. Developmental changes of taste bud distribution on the soft palate, fungiform, foliate and circumvallate papillae in the mouse oral ca...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2008.01.005
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:Detecting the distribution and intensity of NLRP3, NLRP1 and AIM2 expression in different types of periodontitis gingival tissues. MATERIALS AND METHODS:A total of 65 gingival tissues were collected from clinic and been divided into three groups: patients with chronic periodontitis (CP), patients with genera...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.03.005
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:Few clinical techniques are available for the description of mechano-nociceptive function in human periodontal tissues. The objective of this study was to test a new technique for assessment of periodontal mechano-nociceptive function by direct application of controlled forces to the teeth and quantify site, ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2016.07.012
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:MicroRNAs (miRNAs) are recognised as important regulators of a variety of fundamental biologic processes. Our study was undertaken to examine the role of MicroRNA-203 (miR-203) in modulating nitric oxide (NO) expression in female Sprague-Dawley rat mandibular condylar chondrocytes (MCCs) via targeting transie...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.08.013
更新日期:2013-02-01 00:00:00
abstract::This study sought to localize immunohistochemically Janus kinase (Jak) and Tyk isoforms and STAT-1 in association with events involved in early dentine and enamel formation in the rat molar. The Jaks and STATs (signal transducers and activators of transcription) are key signal-transduction pathway components in the cy...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(96)00048-9
更新日期:1996-10-01 00:00:00
abstract::Samples of gingival crevicular fluid (GCF) were collected in 30 volunteers with inflamed gingiva, using either capillary tubes (cGCF) or Durapore strips (sGCF). They were examined, together with samples of serum from the same patients, by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and/or by two-dimensi...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(96)00076-3
更新日期:1996-08-01 00:00:00
abstract:OBJECTIVE:To test the hypothesis that experimental occlusal interferences increase the nerve growth factor (NGF) levels in periodontal tissues and cause an up-regulation of preprotachykinin-A (PPTA) mRNA. BACKGROUND:NGF is related to hyperalgesia and inflammation. PPTA mRNA, a primer of substance P, is a possible fact...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.08.007
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVE:The biofilm of Streptococcus mutans is associated with induction of dental caries. Also, they produce glucan as an extracellular polysaccharide through glucosyltransferases and help the formation of cariogenic biofilm. β-caryophyllene has been used for therapeutic agent in traditional medicine and has antimic...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2018.01.009
更新日期:2018-04-01 00:00:00
abstract::Peptidoglycans and cells walls from Actinomyces viscosus, Staphylococcus aureus, and group A streptococcus were compared for their relative abilities to activate the alternate complement pathway (ACP). On the dry-weight basis, the peptidoglycan from A. viscosus was 3.5 times more active than group A streptococcal pept...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(83)90066-3
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to develop a composite scaffold with the optimal poly(lactic-co-glycolic acid) (PLGA) and bioactive glass proportions to provide an environment for bone tissue regeneration and repair. DESIGN:PLGA-bioactive glass composite scaffolds were prepared using a salt-leaching technique with...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2021.105041
更新日期:2021-01-08 00:00:00
abstract:INTRODUCTION:Covalently bound functional GAGs orchestrate tissue mechanics through time-dependent characteristics. OBJECTIVE:The role of specific glycosaminoglycans (GAGs) at the ligament-cementum and cementum-dentin interfaces within a human periodontal complex were examined. Matrix swelling and resistance to compres...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.11.021
更新日期:2016-03-01 00:00:00
abstract::Pulp tissues isolated from rats treated with either colchicine (0.5, 0.75 or 1.0 mg/kg) or beta-lumicolchicine (1.0 mg/kg) for 24 h were incubated in Tyrode buffer. Prostaglandin (PG) I2 and thromboxane (TX) A2 released into the medium were determined by radioimmunoassay as their stable metabolites, 6-keto-PGF1 alpha ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(88)90063-5
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study is to clarify the functional significance of heat-shock protein (HSP)-25 during tooth development. DESIGN:We compared the expression of HSP-25 in the dental epithelial and mesenchymal cells with their proliferative activity during odontogenesis in rat molars on postnatal days 1-100 by ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2005.09.007
更新日期:2006-05-01 00:00:00