Identification of a lipoarabinomannan-like lipoglycan in Corynebacterium matruchotii.

Abstract:

:The oral organism Corynebacterium matruchotii was investigated for the presence of lipoteichoic acid, as this common polyanionic macroamphiphilic component of Gram-positive bacteria has been implicated in phenomena related to calcium binding. Phenol-water extraction followed by a small-scale, hydrophobic-interaction chromatography step yielded carbohydrate-containing preparations that were distinguished from lipoteichoic acid by their low phosphorus content. Subsequently, large-scale phenol-water extracts from each of three strains of C. matruchotii were purified by hydrophobic-interaction chromatography and shown to contain a heterogeneous lipoglycan fraction. The major fatty acids present were the same as for the whole-cell fatty acid profiles but differed in their relative amounts. Qualitative analysis of the lipoglycan fractions revealed similarities of carbohydrate composition with a previously characterized lipoglycan fraction from C. diphtheriae and with the lipoarabinomannan/lipomannans found in the genus Mycobacterium. The carbohydrate composition and the low phosphorus content indicated that lipoteichoic acid was absent from C. matruchotii. The calcium-binding properties of C. matruchotii therefore cannot be attributed to lipoteichoic acid.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Sutcliffe IC

doi

10.1016/0003-9969(95)00086-0

subject

Has Abstract

pub_date

1995-12-01 00:00:00

pages

1119-24

issue

12

eissn

0003-9969

issn

1879-1506

pii

0003996995000860

journal_volume

40

pub_type

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