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A protein inhibitor of calmodulin-regulated cyclic nucleotide phosphodiesterase in amphibian ovaries.

Abstract：

:The cytosol fraction of an extract of Xenopus laevis ovaries contains a protein inhibitor that can specifically block the activation of calmodulin-sensitive cyclic nucleotide phosphodiesterase (PDE I) found in that tissue. This inhibitor was purified by DEAE-cellulose chromatography, gel filtration on Sephacryl S-200, and affinity chromatography on calmodulin-Sepharose. It has a molecular weight of approximately 90,000, and is heat-labile and susceptible to inactivation by chymotrypsin. The inhibitor blocks calmodulin activation of cyclic nucleotide phosphodiesterases from amphibian ovary and bovine brain and of the myosin light chain kinase from rabbit smooth muscle, but does not affect the activity of a calmodulin-insensitive cyclic nucleotide phosphodiesterase. The inhibitor not only affects the activation of Xenopus PDE I and of the bovine brain phosphodiesterase by calmodulin, but also inhibits the stimulation of these enzymes by lysophosphatidylcholine. The inhibitor also acts on PDE I activated by partial tryptic proteolysis, but the enzyme fully activated by trypsin is only slightly susceptible to inhibition by this protein. The inhibition of PDE I activation caused by this ovarian factor can be reversed by adding excess amounts of calmodulin or lysophosphatidylcholine. The presence of this inhibitor provides a possible explanation for the previously observed inactivity of PDE I in vivo.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Jedlicki E,Orellana O,Allende CC,Allende JE

doi

10.1016/0003-9861(85)90377-7

subject

Has Abstract

pub_date

1985-08-15 00:00:00

pages

215-24

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(85)90377-7

journal_volume

241

pub_type

杂志文章

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